C8a (NM_001290645) Mouse Untagged Clone

CAT#: MC228241

C8a (untagged) - Mouse complement component 8, alpha polypeptide (C8a), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: C8a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228241 representing NM_001290645
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACCCTGGGGTAACTGCACAAGAGAAGGTGAACCATACCGATACCGGAGCCTCTTGCAGCCAAGCA
  AGTTTGGAGGAACCATCTGCAGTGGTGATATCTGGGATGAGGCCAGCTGTGACAGCCCCACACCTTGTCT
  AAGGCAAGCACAGTGTGGACAGGATTTCCAGTGTAGAGAGACAGGTCGCTGCCTGAAACGCCACCTGGTG
  TGTAACGGAGATAACGACTGCCTGGATGGCTCTGATGAGTCCGACTGTGAAGATGTCAGGGTCACTGAGG
  ATGATTGCCATCAGTATGAACCAATCCCAGGATCAGAAAGGGCAGCCTTGGGGTACAACATCCTGACTCA
  AGAGGAAGCTCAGAGTGTTTACGATGCTAAGTATTATGGGGGCCAGTGTGAAACTGTGTACAATGGAGAC
  TGGAGAAAGCTGCGGTACGACCCCACCTGTGAGCGCCTCTACTATGGGGAGGATGAGAAATACTTTCGGA
  AGCCCTACAACTTCCTTAAGTACCACTTTGAAGCCCTGGCGGATACTTCAATCTCCTCAGAGTTTTATGA
  TGATGCGAATGATCTTTTTTTTCACATAAAGAATGGCAAGTCCCACTCAGCTGGTGTAACCGTTGGCGTA
  GCCCCAGTAAAAAGTCCTGTCTCAATAGAGGTGACTGGATCCGGGTCCAAAGCATCTTCATTCCTGAACA
  AGTTAAACAAGTATAATGAGAAGAGATATGGCTTCATGAGAGTGTCTACAAAGATACAGACTGCACAGTT
  TAAGATGAGAAGGAACAATATTGTGCTGGATGAAGGAATGCTGCAGTCATTAATGGAGCTCCCAGAGCAG
  TTCAACTATGGCATGTACGCCAAGTTCATCAATGACTATGGCACCCACTACATCACCTCTGGAACCATGG
  GTGGCATTTATGAATATGTTATGGTGCTTGACAAAGAGAAAATGAAAACTGAAGGTACTACTGTGGATGA
  AGTCCAAAAATGCATTGGCGGTGGCATTGGCATAGGAATTAAGGATAGTACAATTGAAGGAGTAGGTATA
  TCAGGAGAGTTTTGTGAAAACTCTGGAGATGGTGACCGTGACATAAGGAAGAAAATCACAGGAGTGGAGG
  ACATCATTTCTCGGGTGCAAGGTGGAAGTTCTGTCTGGGGCAGTGTCTTGACACACAACAGCAGTGCCAT
  TACATATCAGTCCTGGGGGAGATCGTTAAAGTATAACCCTGTTGTTATCGATTTTGAGATGCAGCCTATC
  TACCAGCTACTGCGGCACACAAACTTGGGACCCCTGGAGACGAAACGTCAGAACCTGCGACGGGCTTTGG
  ACCAGTACCTGATGGAATTTAATGCCTGTCGTTGTGGGCCCTGCTTCAACAATGGGGAACCCATACTTGA
  CGGCACCAACTGCCGGTGTCAGTGTAGTATGGGCCGGCAGGGCCTGGCCTGTGAACGCACGGTGATAGAG
  GGGCTCAAAGACTTCAAAGCCGCTGGCCACTGGAGCTGCTGGAGTTCCTGGTCAGAGTGCAGAGGAGGTT
  CCCAGGAGAGGAGGAGACAGTGCAACAACCCACCACCCAAGAATGGAGGTACCCCATGCCTAGGGAGGAA
  CCTGCAGACCCAGGCCTGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290645
• Insert Size: 1632 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290645.2, NP_001277574.1
• RefSeq Size: 3542 bp
• RefSeq ORF: 1632 bp
• Locus ID: 230558
• Cytogenetics: 4 C6
• Gene Summary: This gene encodes the alpha subunit of complement component C8 that participates in the assembly of the complement membrane attack complex. The encoded preproprotein undergoes proteolytic processing to generate the alpha subunit, which associates with the beta and gamma subunits to form a trimeric complement component, C8. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. This gene is located adjacent to the gene encoding the beta subunit. [provided by RefSeq, Oct 2015]
  Transcript Variant: This variant (2) lacks an alternate exon in the 5' coding region and initiates translation at an alternate start codon, compared to variant 1. It encodes isoform 2, which is shorter and has a distinct N-terminus, compared to isoform 1.