Tyrp1 (NM_001282015) Mouse Tagged ORF Clone

CAT#: MR230427

Tyrp1 (myc-DDK-tagged) - Mouse tyrosinase-related protein 1 (Tyrp1), transcript variant 3

Product Images

Circular map for MR230427

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tyrp1
• Synonyms: b; brown; isa; Oca3; TRP-1; TRP1; Tyrp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230427 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAATCTTACAACGTCCTCCCCCTAGCCTATATCTCCCTTTTCCTGATGCTGTTTTATCAGGTTTGGG
  CTCAGTTTCCACGAGAGTGTGCCAATATTGAGGCTCTGAGACGTGGGGTGTGTTGCCCAGACCTGCTCCC
  TTCCTCTGGACCGGGGACTGACCCTTGTGGCTCATCATCAGGAAGAGGCAGGTGTGTGGCTGTGATTGCA
  GACTCCCGACCCCACAGCCGCCATTATCCCCACGATGGTAAAGATGACCGAGAAGCCTGGCCTCTGAGGT
  TCTTTAATAGAACATGTCAGTGCAATGATAATTTCTCAGGACACAACTGTGGGACTTGCCGTCCTGGGTG
  GAGAGGAGCTGCATGCAACCAGAAAATTCTCACAGTCAGGAGAAATCTTCTAGACTTAAGTCCAGAAGAA
  AAGAGCCACTTTGTCAGGGCCTTGGATATGGCGAAGCGCACAACTCACCCTCAATTTGTCATTGCCACAA
  GGAGGTTAGAAGACATACTGGGACCAGATGGCAACACACCACAATTTGAGAACATTTCCGTTTATAACTA
  CTTTGTTTGGACACACTATTATTCAGTCAAAAAAACCTTCCTCGGGACAGGACAGGAAAGCTTTGGGGAT
  GTGGATTTCTCTCACGAAGGACCCGCTTTTCTCACATGGCACAGGTACCATCTGCTGCAGCTGGAGAGAG
  ACATGCAGGAGATGCTGCAGGAGCCTTCTTTCTCCCTTCCTTACTGGAATTTTGCAACTGGGAAAAACGT
  CTGCGATGTCTGCACTGATGACTTGATGGGATCCAGAAGCAACTTCGATTCTACTCTTATAAGCCCCAAC
  TCTGTCTTTTCTCAATGGAGAGTGGTCTGTGAATCCTTGGAAGAGTACGATACCCTGGGAACACTTTGTA
  ACAGCACTGAGGGTGGACCAATCAGGAGAAACCCAGCTGGAAATGTAGGGAGACCAGCAGTGCAGCGTCT
  TCCTGAGCCACAGGATGTCACTCAGTGCTTGGAGGTCCGTGTATTTGACACACCTCCTTTTTATTCCAAT
  TCTACAGACAGTTTTCGAAATACAGTGGAAGGTTACAGTGCTCCCACGGGAAAATATGACCCTGCTGTTC
  GAAGCCTTCACAACCTGGCCCACCTCTTCCTGAATGGAACGGGAGGACAAACCCATTTGTCTCCCAATGA
  TCCTATTTTTGTCCTCCTGCACACTTTCACTGATGCGGTCTTTGACGAATGGCTAAGGAGGTATAACGCC
  GATATTTCTACCTTCCCGTTGGAAAACGCACCTATTGGACATAACAGGCAATACAACATGGTGCCATTCT
  GGCCTCCAGTTACCAACACAGAAATGTTTGTTACTGCTCCAGACAATCTGGGATATGCTTATGAAGTTCA
  ATGGCCAGGTCAGGAGTTTACTGTATCTGAAATCATTACCATTGCTGTAGTGGCTGCGTTGTTACTTGTA
  GCTGCCATTTTCGGGGTTGCTTCTTGTCTGATCCGTTCTAGAAGCACCAAGAATGAAGCCAACCAGCCTC
  TCCTCACTGATCACTATCAACGCTATGCTGAGGACTATGAGGAGCTCCCGAATCCTAACCACTCCATGGT
  C
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230427 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKSYNVLPLAYISLFLMLFYQVWAQFPRECANIEALRRGVCCPDLLPSSGPGTDPCGSSSGRGRCVAVIA
  DSRPHSRHYPHDGKDDREAWPLRFFNRTCQCNDNFSGHNCGTCRPGWRGAACNQKILTVRRNLLDLSPEE
  KSHFVRALDMAKRTTHPQFVIATRRLEDILGPDGNTPQFENISVYNYFVWTHYYSVKKTFLGTGQESFGD
  VDFSHEGPAFLTWHRYHLLQLERDMQEMLQEPSFSLPYWNFATGKNVCDVCTDDLMGSRSNFDSTLISPN
  SVFSQWRVVCESLEEYDTLGTLCNSTEGGPIRRNPAGNVGRPAVQRLPEPQDVTQCLEVRVFDTPPFYSN
  STDSFRNTVEGYSAPTGKYDPAVRSLHNLAHLFLNGTGGQTHLSPNDPIFVLLHTFTDAVFDEWLRRYNA
  DISTFPLENAPIGHNRQYNMVPFWPPVTNTEMFVTAPDNLGYAYEVQWPGQEFTVSEIITIAVVAALLLV
  AAIFGVASCLIRSRSTKNEANQPLLTDHYQRYAEDYEELPNPNHSMV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282015
• ORF Size: 1611 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282015.1, NP_001268944.1
• RefSeq Size: 2734 bp
• RefSeq ORF: 1614 bp
• Locus ID: 22178
• UniProt ID: P07147
• Cytogenetics: 4 37.89 cM
• MW: 60.8 kDa
• Gene Summary: Plays a role in melanin biosynthesis (PubMed:2245916). Catalyzes the oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid (PubMed:7813420). May regulate or influence the type of melanin synthesized (PubMed:7813420, PubMed:2245916). Also to a lower extent, capable of hydroxylating tyrosine and producing melanin (PubMed:1537333).[UniProtKB/Swiss-Prot Function]