Tyrp1 (NM_001282015) Mouse Untagged Clone

CAT#: MC228216

Tyrp1 (untagged) - Mouse tyrosinase-related protein 1 (Tyrp1), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tyrp1
• Synonyms: b; brown; isa; Oca3; TRP-1; TRP1; Tyrp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228216 representing NM_001282015
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAATCTTACAACGTCCTCCCCCTAGCCTATATCTCCCTTTTCCTGATGCTGTTTTATCAGGTTTGGG
  CTCAGTTTCCACGAGAGTGTGCCAATATTGAGGCTCTGAGACGTGGGGTGTGTTGCCCAGACCTGCTCCC
  TTCCTCTGGACCGGGGACTGACCCTTGTGGCTCATCATCAGGAAGAGGCAGGTGTGTGGCTGTGATTGCA
  GACTCCCGACCCCACAGCCGCCATTATCCCCACGATGGTAAAGATGACCGAGAAGCCTGGCCTCTGAGGT
  TCTTTAATAGAACATGTCAGTGCAATGATAATTTCTCAGGACACAACTGTGGGACTTGCCGTCCTGGGTG
  GAGAGGAGCTGCATGCAACCAGAAAATTCTCACAGTCAGGAGAAATCTTCTAGACTTAAGTCCAGAAGAA
  AAGAGCCACTTTGTCAGGGCCTTGGATATGGCGAAGCGCACAACTCACCCTCAATTTGTCATTGCCACAA
  GGAGGTTAGAAGACATACTGGGACCAGATGGCAACACACCACAATTTGAGAACATTTCCGTTTATAACTA
  CTTTGTTTGGACACACTATTATTCAGTCAAAAAAACCTTCCTCGGGACAGGACAGGAAAGCTTTGGGGAT
  GTGGATTTCTCTCACGAAGGACCCGCTTTTCTCACATGGCACAGGTACCATCTGCTGCAGCTGGAGAGAG
  ACATGCAGGAGATGCTGCAGGAGCCTTCTTTCTCCCTTCCTTACTGGAATTTTGCAACTGGGAAAAACGT
  CTGCGATGTCTGCACTGATGACTTGATGGGATCCAGAAGCAACTTCGATTCTACTCTTATAAGCCCCAAC
  TCTGTCTTTTCTCAATGGAGAGTGGTCTGTGAATCCTTGGAAGAGTACGATACCCTGGGAACACTTTGTA
  ACAGCACTGAGGGTGGACCAATCAGGAGAAACCCAGCTGGAAATGTAGGGAGACCAGCAGTGCAGCGTCT
  TCCTGAGCCACAGGATGTCACTCAGTGCTTGGAGGTCCGTGTATTTGACACACCTCCTTTTTATTCCAAT
  TCTACAGACAGTTTTCGAAATACAGTGGAAGGTTACAGTGCTCCCACGGGAAAATATGACCCTGCTGTTC
  GAAGCCTTCACAACCTGGCCCACCTCTTCCTGAATGGAACGGGAGGACAAACCCATTTGTCTCCCAATGA
  TCCTATTTTTGTCCTCCTGCACACTTTCACTGATGCGGTCTTTGACGAATGGCTAAGGAGGTATAACGCC
  GATATTTCTACCTTCCCGTTGGAAAACGCACCTATTGGACATAACAGGCAATACAACATGGTGCCATTCT
  GGCCTCCAGTTACCAACACAGAAATGTTTGTTACTGCTCCAGACAATCTGGGATATGCTTATGAAGTTCA
  ATGGCCAGGTCAGGAGTTTACTGTATCTGAAATCATTACCATTGCTGTAGTGGCTGCGTTGTTACTTGTA
  GCTGCCATTTTCGGGGTTGCTTCTTGTCTGATCCGTTCTAGAAGCACCAAGAATGAAGCCAACCAGCCTC
  TCCTCACTGATCACTATCAACGCTATGCTGAGGACTATGAGGAGCTCCCGAATCCTAACCACTCCATGGT
  CTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001282015
• Insert Size: 1614 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282015.1, NP_001268944.1
• RefSeq Size: 2734 bp
• RefSeq ORF: 1614 bp
• Locus ID: 22178
• UniProt ID: P07147
• Cytogenetics: 4 37.89 cM
• Gene Summary: Plays a role in melanin biosynthesis (PubMed:2245916). Catalyzes the oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid (PubMed:7813420). May regulate or influence the type of melanin synthesized (PubMed:7813420, PubMed:2245916). Also to a lower extent, capable of hydroxylating tyrosine and producing melanin (PubMed:1537333).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1, 2, and 3 encode the same protein.