Dok1 (NM_001291799) Mouse Tagged ORF Clone

CAT#: MR229806

Dok1 (myc-DDK-tagged) - Mouse docking protein 1 (Dok1), transcript variant 2

Product Images

Circular map for MR229806

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dok1
• Synonyms: AW557123; p62DOK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR229806 representing NM_001291799
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATACGCCTGGCTGAATGTGTGAGCGTGGTGCCGGTGACTGTGGAGAGTCCCCCTGAGCCCGGCGCTG
  TTGCCTTCCGCCTGGACACCGCACAGCGCTCGCACCTGCTGGCGGCGGACGCCGTATCCAGCACCGCCTG
  GGTGCAGACTTTATGCAGAACCGCCTTTCCGAAAGGCGGCTGGGCTTTGGCGCAGACGGAGAACCAACCT
  AAGTTTTCTGCCTTGGAGATGCTGGAGAATTCGCTGTACAGCCCCACCTGGGAAGGATCCCAGTTCTGGG
  TAACCTCGCAGAAGACCGAGGCTTCTGAACGCTGCGGCTTGCAAGGCTCCTACATACTGAGGGTGGAAGC
  TGAGAAGCTGACTCTCCTGACTTTGGGTGCGCAGAGTCAAATCCTGGAGCCGCTCCTTTTCTGGCCCTAC
  ACTCTGTTGCGTCGCTATGGCCGGGACAAGGTAATGTTCTCCTTTGAAGCTGGTCGCCGCTGCCCCTCAG
  GCCCTGGGACCTTCACCTTCCAGACTTCTCAGGGAAATGACATCTTTCAGGCAGTTGAGGCTGCCATCCA
  GCAGCAGAAAGCCCAAGGAAAGGTGGGCCAGGCACAGGATATCCTCAGAACTGACTCCCATGATGGGGAG
  ACAGAGGGGAAGACAGTTCCCCCTCCTGTTCCCCAGGACCCCCTGGGCAGCCCTCCAGCCCTATATGCGG
  AGCCTTTAGACTCCTTGCGAATTCCTCCAGGCCCTTCTCAGGACTCTGTATATTCAGACCCCCTGGGCAG
  CACCCCTGCTGGGGCAGGGGAAGGGGTGCATTCCAAGAAACCTCTCTATTGGGATTTGTATGGGCATGTG
  CAGCAGCAGTTACTGAAAACCAAGCTGACAGACTCCAAAGAGGACCCCATCTATGATGAACCTGAAGGCC
  TGGCCCCCGCCCCTCCCCGGGGCCTTTATGATCTGCCTCAGGAGCCTCGGGATGCATGGTGGTGCCAGGC
  TCGGCTGAAGGAAGAGGGCTATGAGCTCCCTTACAACCCTGCCACCGATGACTATGCTGTGCCACCTCCC
  CGGAGCCCAAAGCCTGCTCCTGCCCCCAAGCCACAGGGCTTGATCCTTCCCGAATCGGGTACCACTCGTG
  GCAGTGGCAGCAAAGGCTTCAGCTCAGATACAGCTCTGTACAGCCAGGTCCAGAAAAGTGGGACCTCAGG
  GGCTTGGGACTGTGGACTCTCTAAAGTAGGGAATGACAGGGCGGGGGTCAAGTCTGAGGGTTCCACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR229806 representing NM_001291799
  Red=Cloning site Green=Tags(s)
  
  MIRLAECVSVVPVTVESPPEPGAVAFRLDTAQRSHLLAADAVSSTAWVQTLCRTAFPKGGWALAQTENQP
  KFSALEMLENSLYSPTWEGSQFWVTSQKTEASERCGLQGSYILRVEAEKLTLLTLGAQSQILEPLLFWPY
  TLLRRYGRDKVMFSFEAGRRCPSGPGTFTFQTSQGNDIFQAVEAAIQQQKAQGKVGQAQDILRTDSHDGE
  TEGKTVPPPVPQDPLGSPPALYAEPLDSLRIPPGPSQDSVYSDPLGSTPAGAGEGVHSKKPLYWDLYGHV
  QQQLLKTKLTDSKEDPIYDEPEGLAPAPPRGLYDLPQEPRDAWWCQARLKEEGYELPYNPATDDYAVPPP
  RSPKPAPAPKPQGLILPESGTTRGSGSKGFSSDTALYSQVQKSGTSGAWDCGLSKVGNDRAGVKSEGST
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001291799
• ORF Size: 1257 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291799.1, NP_001278728.1
• RefSeq Size: 1809 bp
• RefSeq ORF: 1260 bp
• Locus ID: 13448
• UniProt ID: P97465
• Cytogenetics: 6 35.94 cM
• MW: 45.8 kDa
• Gene Summary: DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3 (By similarity).[UniProtKB/Swiss-Prot Function]