Dok1 (NM_001291799) Mouse Untagged Clone

CAT#: MC227595

Dok1 (untagged) - Mouse docking protein 1 (Dok1), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dok1
• Synonyms: AW557123; p62DOK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227595 representing NM_001291799
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATACGCCTGGCTGAATGTGTGAGCGTGGTGCCGGTGACTGTGGAGAGTCCCCCTGAGCCCGGCGCTG
  TTGCCTTCCGCCTGGACACCGCACAGCGCTCGCACCTGCTGGCGGCGGACGCCGTATCCAGCACCGCCTG
  GGTGCAGACTTTATGCAGAACCGCCTTTCCGAAAGGCGGCTGGGCTTTGGCGCAGACGGAGAACCAACCT
  AAGTTTTCTGCCTTGGAGATGCTGGAGAATTCGCTGTACAGCCCCACCTGGGAAGGATCCCAGTTCTGGG
  TAACCTCGCAGAAGACCGAGGCTTCTGAACGCTGCGGCTTGCAAGGCTCCTACATACTGAGGGTGGAAGC
  TGAGAAGCTGACTCTCCTGACTTTGGGTGCGCAGAGTCAAATCCTGGAGCCGCTCCTTTTCTGGCCCTAC
  ACTCTGTTGCGTCGCTATGGCCGGGACAAGGTAATGTTCTCCTTTGAAGCTGGTCGCCGCTGCCCCTCAG
  GCCCTGGGACCTTCACCTTCCAGACTTCTCAGGGAAATGACATCTTTCAGGCAGTTGAGGCTGCCATCCA
  GCAGCAGAAAGCCCAAGGAAAGGTGGGCCAGGCACAGGATATCCTCAGAACTGACTCCCATGATGGGGAG
  ACAGAGGGGAAGACAGTTCCCCCTCCTGTTCCCCAGGACCCCCTGGGCAGCCCTCCAGCCCTATATGCGG
  AGCCTTTAGACTCCTTGCGAATTCCTCCAGGCCCTTCTCAGGACTCTGTATATTCAGACCCCCTGGGCAG
  CACCCCTGCTGGGGCAGGGGAAGGGGTGCATTCCAAGAAACCTCTCTATTGGGATTTGTATGGGCATGTG
  CAGCAGCAGTTACTGAAAACCAAGCTGACAGACTCCAAAGAGGACCCCATCTATGATGAACCTGAAGGCC
  TGGCCCCCGCCCCTCCCCGGGGCCTTTATGATCTGCCTCAGGAGCCTCGGGATGCATGGTGGTGCCAGGC
  TCGGCTGAAGGAAGAGGGCTATGAGCTCCCTTACAACCCTGCCACCGATGACTATGCTGTGCCACCTCCC
  CGGAGCCCAAAGCCTGCTCCTGCCCCCAAGCCACAGGGCTTGATCCTTCCCGAATCGGGTACCACTCGTG
  GCAGTGGCAGCAAAGGCTTCAGCTCAGATACAGCTCTGTACAGCCAGGTCCAGAAAAGTGGGACCTCAGG
  GGCTTGGGACTGTGGACTCTCTAAAGTAGGGAATGACAGGGCGGGGGTCAAGTCTGAGGGTTCCACCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001291799
• Insert Size: 1260 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291799.1, NP_001278728.1
• RefSeq Size: 1809 bp
• RefSeq ORF: 1260 bp
• Locus ID: 13448
• UniProt ID: P97465
• Cytogenetics: 6 35.94 cM
• Gene Summary: DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3 (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate splice junction at the 5' end of an exon and uses a downstream start codon compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1.