Spns2 (NM_001276383) Mouse Tagged ORF Clone

CAT#: MR229697

Spns2 (myc-DDK-tagged) - Mouse spinster homolog 2 (Spns2), transcript variant 2

Product Images

Circular map for MR229697

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Spns2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR229697 representing NM_001276383
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGCTGCCCCCATCTTTGGCTACCTGGGCGACCGCTTCAATAGAAAGGTGATCCTCAGCTGTGGCA
  TCTTCTTCTGGTCTGCTGTCACCTTCTCGAGTTCTTTCATCCCTCAGCAGTACTTCTGGCTACTAGTCCT
  GTCCCGAGGGCTAGTAGGTATTGGTGAAGCCAGCTACTCCACCATAGCACCCACCATCATTGGCGACTTG
  TTTACCAAGAACACACGCACATTGATGCTATCTGTCTTCTATTTTGCCATCCCCCTGGGCAGTGGCCTGG
  GCTATATCACAGGTTCCAGCGTGAAGCAGGCAGCTGGAGACTGGCATTGGGCCCTGCGGGTTTCCCCCGT
  CCTGGGCATGATCACAGGAACACTCATCCTCATCTTGGTCCCAGCCACTAAGAGAGGCCATGCTGATCAA
  CTTGGGGGGCAGCTCAAAGCACGGACCTCCTGGCTTCGAGACATGAAGGCCCTGATCCGAAACCGCAGTT
  ACGTCTTCTCCTCCCTGGCCACATCCGCTGTGTCCTTCGCCACAGGGGCCCTGGGCATGTGGATTCCCCT
  CTATCTTCACCGTGCTCAAGTTGTACAAAAGACAGCAGAGACTTGCAACAGCCCGCCCTGTGGAGCCAAA
  GACAGCCTCATCTTTGGAGCCATTACCTGCTTTACTGGCTTCCTGGGCGTAGTCACGGGCGCGGGAGCCA
  CTCGTTGGTGCCGCCTGCGGACTCAACGTGCTGACCCATTGGTGTGTGCTGTGGGCATGCTGGGATCCGC
  CATCTTCATCTGCCTCATCTTTGTGGCTGCCAAGACCAGCATTGTAGGCGCTTATATCTGCATTTTTGTT
  GGAGAGACACTGCTGTTTTCTAACTGGGCCATCACTGCAGACATCCTCATGTATGTGGTCATCCCCACTC
  GGCGAGCCACTGCTGTGGCCCTGCAGAGCTTCACCTCCCATCTGCTGGGGGACGCTGGAAGCCCCTACCT
  CATTGGCTTTATCTCGGACCTAATCCGCCAGAGCACCAAGGACTCCCCGCTCTGGGAGTTCCTGAGCCTG
  GGCTATGCCCTCATGCTGTGCCCTTTTGTCGTGGTCCTGGGTGGCATGTTCTTCCTTGCCACTGCTCTCT
  TCTTCCTCAGCGACCGTGCCAAGGCTGAGCAGCAGGTGAACCAGCTGGTGATGCCTCCTGCATCCGTGAA
  AGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR229697 representing NM_001276383
  Red=Cloning site Green=Tags(s)
  
  MVAAPIFGYLGDRFNRKVILSCGIFFWSAVTFSSSFIPQQYFWLLVLSRGLVGIGEASYSTIAPTIIGDL
  FTKNTRTLMLSVFYFAIPLGSGLGYITGSSVKQAAGDWHWALRVSPVLGMITGTLILILVPATKRGHADQ
  LGGQLKARTSWLRDMKALIRNRSYVFSSLATSAVSFATGALGMWIPLYLHRAQVVQKTAETCNSPPCGAK
  DSLIFGAITCFTGFLGVVTGAGATRWCRLRTQRADPLVCAVGMLGSAIFICLIFVAAKTSIVGAYICIFV
  GETLLFSNWAITADILMYVVIPTRRATAVALQSFTSHLLGDAGSPYLIGFISDLIRQSTKDSPLWEFLSL
  GYALMLCPFVVVLGGMFFLATALFFLSDRAKAEQQVNQLVMPPASVKV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001276383
• ORF Size: 1194 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001276383.1, NP_001263312.1
• RefSeq Size: 2909 bp
• RefSeq ORF: 1197 bp
• Locus ID: 216892
• Cytogenetics: 11 B4
• MW: 43.5 kDa
• Gene Summary: Sphingolipid transporter required for migration of myocardial precursors. Transports sphingosine 1-phosphate (S1P), a secreted lipid mediator that plays critical roles in cardiovascular, immunological, and neural development and function. Mediates the export of S1P from cells in the extraembryonic yolk syncytial layer (YSL), thereby regulating myocardial precursor migration (By similarity).[UniProtKB/Swiss-Prot Function]