Spns2 (NM_001276383) Mouse Untagged Clone

CAT#: MC227486

Spns2 (untagged) - Mouse spinster homolog 2 (Spns2), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Spns2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227486 representing NM_001276383
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGCTGCCCCCATCTTTGGCTACCTGGGCGACCGCTTCAATAGAAAGGTGATCCTCAGCTGTGGCA
  TCTTCTTCTGGTCTGCTGTCACCTTCTCGAGTTCTTTCATCCCTCAGCAGTACTTCTGGCTACTAGTCCT
  GTCCCGAGGGCTAGTAGGTATTGGTGAAGCCAGCTACTCCACCATAGCACCCACCATCATTGGCGACTTG
  TTTACCAAGAACACACGCACATTGATGCTATCTGTCTTCTATTTTGCCATCCCCCTGGGCAGTGGCCTGG
  GCTATATCACAGGTTCCAGCGTGAAGCAGGCAGCTGGAGACTGGCATTGGGCCCTGCGGGTTTCCCCCGT
  CCTGGGCATGATCACAGGAACACTCATCCTCATCTTGGTCCCAGCCACTAAGAGAGGCCATGCTGATCAA
  CTTGGGGGGCAGCTCAAAGCACGGACCTCCTGGCTTCGAGACATGAAGGCCCTGATCCGAAACCGCAGTT
  ACGTCTTCTCCTCCCTGGCCACATCCGCTGTGTCCTTCGCCACAGGGGCCCTGGGCATGTGGATTCCCCT
  CTATCTTCACCGTGCTCAAGTTGTACAAAAGACAGCAGAGACTTGCAACAGCCCGCCCTGTGGAGCCAAA
  GACAGCCTCATCTTTGGAGCCATTACCTGCTTTACTGGCTTCCTGGGCGTAGTCACGGGCGCGGGAGCCA
  CTCGTTGGTGCCGCCTGCGGACTCAACGTGCTGACCCATTGGTGTGTGCTGTGGGCATGCTGGGATCCGC
  CATCTTCATCTGCCTCATCTTTGTGGCTGCCAAGACCAGCATTGTAGGCGCTTATATCTGCATTTTTGTT
  GGAGAGACACTGCTGTTTTCTAACTGGGCCATCACTGCAGACATCCTCATGTATGTGGTCATCCCCACTC
  GGCGAGCCACTGCTGTGGCCCTGCAGAGCTTCACCTCCCATCTGCTGGGGGACGCTGGAAGCCCCTACCT
  CATTGGCTTTATCTCGGACCTAATCCGCCAGAGCACCAAGGACTCCCCGCTCTGGGAGTTCCTGAGCCTG
  GGCTATGCCCTCATGCTGTGCCCTTTTGTCGTGGTCCTGGGTGGCATGTTCTTCCTTGCCACTGCTCTCT
  TCTTCCTCAGCGACCGTGCCAAGGCTGAGCAGCAGGTGAACCAGCTGGTGATGCCTCCTGCATCCGTGAA
  AGTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001276383
• Insert Size: 1197 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001276383.1, NP_001263312.1
• RefSeq Size: 2909 bp
• RefSeq ORF: 1197 bp
• Locus ID: 216892
• Cytogenetics: 11 B4
• Gene Summary: Sphingolipid transporter required for migration of myocardial precursors. Transports sphingosine 1-phosphate (S1P), a secreted lipid mediator that plays critical roles in cardiovascular, immunological, and neural development and function. Mediates the export of S1P from cells in the extraembryonic yolk syncytial layer (YSL), thereby regulating myocardial precursor migration (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) contains an alternate exon in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.