Slbp (NM_001289725) Mouse Tagged ORF Clone

CAT#: MR228722

Slbp (myc-DDK-tagged) - Mouse stem-loop binding protein (Slbp), transcript variant 3

Product Images

Circular map for MR228722

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slbp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR228722 representing NM_001289725
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTGCAGACCTAGAAGCCCACCCGGTTATGGGAGTCGCCGCGACGGTGGTGCGAGCCCGCGGTCCC
  CTGCGAGATGGAGTCTCGGACGGAAGCGCAGAGCGGACGGAAGGGACCGGAAGCCCGAGGACTCCGAGGA
  AGGCGAGCTCCAGACTGCGGACCACCGACCTGAAAGGTACAAAAGAAAACTGCTCATAAACGACTTTGGG
  AGAGAGAGGAAGTCATCATCGGGAAGTTCTGATTCAAAGGAGTCTATGTCTTCAGTGCCTGCTGATGTGG
  AGACGGATGAAAGTGTCTTGATGAGAAGGCAGAAGCAGATCAACTACGGGAAGAACACTATTGCCTATGA
  TCGATATATTAAAGAGGTGCCCAGACACCTGCGACAACCTGGAATCCATCCCAGGACCCCCAACAAATTT
  AAGAAGTACAGCCGGCGGTCATGGGACCAACAAATTAAACTCTGGAAGGTGGCTTTGCATTTTTGGGATC
  CTCCTGCTGAAGAAGGATGTGATTTGCAAGAAATACAACCTGTAGATCTTGGGGAAATGGAGACTGAATT
  CACAGAAAGCAGCTCTGAGTCTCAGACAAGTTCACAGGATAACTTTGATGTGTATGCTGGCACACCCACC
  AAAGTCAGACACGTGGACTGCCAAGTGGAGGATGAGTTTGATTTGGAAGCTTGTTTAACTGAACCTTTGA
  AAGACTTCTCTGCCATGAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR228722 representing NM_001289725
  Red=Cloning site Green=Tags(s)
  
  MACRPRSPPGYGSRRDGGASPRSPARWSLGRKRRADGRDRKPEDSEEGELQTADHRPERYKRKLLINDFG
  RERKSSSGSSDSKESMSSVPADVETDESVLMRRQKQINYGKNTIAYDRYIKEVPRHLRQPGIHPRTPNKF
  KKYSRRSWDQQIKLWKVALHFWDPPAEEGCDLQEIQPVDLGEMETEFTESSSESQTSSQDNFDVYAGTPT
  KVRHVDCQVEDEFDLEACLTEPLKDFSAMS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001289725
• ORF Size: 720 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289725.1, NP_001276654.1
• RefSeq Size: 1816 bp
• RefSeq ORF: 723 bp
• Locus ID: 20492
• Cytogenetics: 5 B2
• MW: 28 kDa
• Gene Summary: RNA-binding protein involved in the histone pre-mRNA processing. Binds the stem-loop structure of replication-dependent histone pre-mRNAs and contributes to efficient 3'-end processing by stabilizing the complex between histone pre-mRNA and U7 small nuclear ribonucleoprotein (snRNP), via the histone downstream element (HDE). Plays an important role in targeting mature histone mRNA from the nucleus to the cytoplasm and to the translation machinery. Stabilizes mature histone mRNA and could be involved in cell-cycle regulation of histone gene expression (By similarity). Involved in the mechanism by which growing oocytes accumulate histone proteins that support early embryogenesis. Binds to the 5' side of the stem-loop structure of histone pre-mRNAs.[UniProtKB/Swiss-Prot Function]