Nudt7 (NM_001290180) Mouse Tagged ORF Clone

CAT#: MR228516

Nudt7 (myc-DDK-tagged) - Mouse nudix (nucleoside diphosphate linked moiety X)-type motif 7 (Nudt7), transcript variant 3

Product Images

Circular map for MR228516

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nudt7
• Synonyms: 1300007B24Rik; 2210404C19Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR228516 representing NM_001290180
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCGTCAGAACTATTTGCTTCCCGAAGTCTCCTGCTGCTAGTAAGAGGAAGTACTGCAGGGCTGATG
  TCGCGACCTTGTGGACTCCCGGAGCCTGTCAGCTGAAAAGGGAACCTGGAGAAGTCTGCTTCCCTGGAGG
  AAAGAGGGACCCGGTGGACACAGATGACACAGCCACTGCTCTCCGTGAAGCCCAGGAGGAGGTGGGGCTG
  CATCCCCACCAAGTGGAGGTGGTCTCTCACCTGGTGCCATACGTATTTGATAATGATGCACTGGTAACCC
  CCGTAGTGGGTTTTCTAGACCACAACTTCCAGGCCCAACCTAATGCTGATGAAGTGAAGGAAGTCTTCTT
  TGTGCCTTTGGACTATTTCCTCCATCCCCAAGTCTACTACCAGAAGCAAATCACACAGTCTGGCCGTGAT
  TTCATCATGCATTGCTTCGAGTACAAAGACCCTGAGACTGGTGTGAACTACCTAATCCAGGGAATGACCT
  CAAAACTGGCTGTGTTGGTGGCCTTAATTATTTTGGAACAAAGTCCTGCCTTCAAGATTGATTTTGATCT
  CCATGACCTGATACCGTCTTGTGAAAGGACCTTCCTTTGGAGATATTCTTTAAGCAAGTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR228516 representing NM_001290180
  Red=Cloning site Green=Tags(s)
  
  MRVRTICFPKSPAASKRKYCRADVATLWTPGACQLKREPGEVCFPGGKRDPVDTDDTATALREAQEEVGL
  HPHQVEVVSHLVPYVFDNDALVTPVVGFLDHNFQAQPNADEVKEVFFVPLDYFLHPQVYYQKQITQSGRD
  FIMHCFEYKDPETGVNYLIQGMTSKLAVLVALIILEQSPAFKIDFDLHDLIPSCERTFLWRYSLSKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001290180
• ORF Size: 621 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290180.1, NP_001277109.1
• RefSeq Size: 945 bp
• RefSeq ORF: 624 bp
• Locus ID: 67528
• UniProt ID: Q99P30
• Cytogenetics: 8 E1
• MW: 24 kDa
• Gene Summary: Coenzyme A diphosphatase which mediates the cleavage of CoA, CoA esters and oxidized CoA with similar efficiencies, yielding 3',5'-ADP and the corresponding 4'-phosphopantetheine derivative as products. CoA into 3',5'-ADP and 4'-phosphopantetheine. Has no activity toward NDP-sugars, CDP-alcohols, (deoxy)nucleoside 5'-triphosphates, nucleoside 5'-di or monophosphates, diadenosine polyphosphates, NAD, NADH, NADP, NADPH or thymidine-5'-monophospho-p-nitrophenyl ester. May be required to eliminate oxidized CoA from peroxisomes, or regulate CoA and acyl-CoA levels in this organelle in response to metabolic demand. Does not play a role in U8 snoRNA decapping activity. Binds U8 snoRNA.[UniProtKB/Swiss-Prot Function]