Tyw5 (NM_001302963) Mouse Tagged ORF Clone
CAT#: MR228295
- TrueORF®
Tyw5 (myc-DDK-tagged) - Mouse tRNA-yW synthesizing protein 5 (Tyw5), transcript variant 5
"NM_001302963" in other vectors (1)
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USD 198.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Tyw5 |
Synonyms | 1110034B05Rik |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR228295 representing NM_001302963
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCTTCAGAGAGGAGCAGTTCTTTTCCAGTGTTTTTCGAATTAGTTCACCTGGATTACAGCTCTGGA CTCACTATGATGTAATGGATAACTTTTTAATACAAGTGACAGGAAAGAAGCGAATTACACTGTTCAATCC TCGGGATGCACAATATTTATATTTATCAGTGCTGAATATCGACAGCCCAGACCTGGATAAATACCCACTC TTTCCTAAAGCAAGGAGGTATGAGTGCTCCCTGGAAGCTGGAGATGTCCTCTTCATTCCTGCTTTATGGT TCCATAATGTAGTTTCTGAAGAGTTTGGAGTGGGGGTGAATATCTTCTGGAAGCACCTTCCATCGGAATG CTATGACACAACAGATACCTATGGCAACAAAGATCCTGTAGCGGCATCCAGAGCTGTGCAGATCTTGGAC AGAGCTTTGAAAACACTGGCTGAATTACCAGAGGAATACAGGGACTTCTATGCACGCCAAATGGTCTTAC GTATTCAAGACAAAGCCTACAGCAAGAACTTTGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR228295 representing NM_001302963
Red=Cloning site Green=Tags(s) MFFREEQFFSSVFRISSPGLQLWTHYDVMDNFLIQVTGKKRITLFNPRDAQYLYLSVLNIDSPDLDKYPL FPKARRYECSLEAGDVLFIPALWFHNVVSEEFGVGVNIFWKHLPSECYDTTDTYGNKDPVAASRAVQILD RALKTLAELPEEYRDFYARQMVLRIQDKAYSKNFE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001302963 |
ORF Size | 525 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001302963.1, NP_001289892.1 |
RefSeq Size | 1399 bp |
RefSeq ORF | 528 bp |
Locus ID | 68736 |
Cytogenetics | 1 C1.3 |
MW | 21 kDa |
Gene Summary | tRNA hydroxylase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the hydroxylation of 7-(a-amino-a-carboxypropyl)wyosine (yW-72) into undermodified hydroxywybutosine (OHyW*). OHyW* being further transformed into hydroxywybutosine (OHyW) by LCMT2/TYW4. OHyW is a derivative of wybutosine found in higher eukaryotes (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC226084 | Tyw5 (untagged) - Mouse tRNA-yW synthesizing protein 5 (Tyw5), transcript variant 5 |
USD 330.00 |
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