Tyw5 (NM_001302963) Mouse Untagged Clone

CAT#: MC226084

Tyw5 (untagged) - Mouse tRNA-yW synthesizing protein 5 (Tyw5), transcript variant 5


  "NM_001302963" in other vectors (1)

Reconstitution Protocol

USD 330.00

2 Weeks*

Size
    • 10 ug

Product Images

Frequently bought together (3)
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Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Symbol Tyw5
Synonyms 1110034B05Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC226084 representing NM_001302963
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTCTTCAGAGAGGAGCAGTTCTTTTCCAGTGTTTTTCGAATTAGTTCACCTGGATTACAGCTCTGGA
CTCACTATGATGTAATGGATAACTTTTTAATACAAGTGACAGGAAAGAAGCGAATTACACTGTTCAATCC
TCGGGATGCACAATATTTATATTTATCAGTGCTGAATATCGACAGCCCAGACCTGGATAAATACCCACTC
TTTCCTAAAGCAAGGAGGTATGAGTGCTCCCTGGAAGCTGGAGATGTCCTCTTCATTCCTGCTTTATGGT
TCCATAATGTAGTTTCTGAAGAGTTTGGAGTGGGGGTGAATATCTTCTGGAAGCACCTTCCATCGGAATG
CTATGACACAACAGATACCTATGGCAACAAAGATCCTGTAGCGGCATCCAGAGCTGTGCAGATCTTGGAC
AGAGCTTTGAAAACACTGGCTGAATTACCAGAGGAATACAGGGACTTCTATGCACGCCAAATGGTCTTAC
GTATTCAAGACAAAGCCTACAGCAAGAACTTTGAGTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001302963
Insert Size 528 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001302963.1, NP_001289892.1
RefSeq Size 1399 bp
RefSeq ORF 528 bp
Locus ID 68736
Cytogenetics 1 C1.3
Gene Summary tRNA hydroxylase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the hydroxylation of 7-(a-amino-a-carboxypropyl)wyosine (yW-72) into undermodified hydroxywybutosine (OHyW*). OHyW* being further transformed into hydroxywybutosine (OHyW) by LCMT2/TYW4. OHyW is a derivative of wybutosine found in higher eukaryotes (By similarity).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (5) lacks an alternate internal exon, uses an alternate in-frame splice site in the 5' coding region, and uses an in-frame downstream start codon compared to variant 4. The resulting protein (isoform 5) is shorter than isoform 4.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.