Ldha (NM_001136069) Mouse Tagged ORF Clone

CAT#: MR227451

Ldha (Myc-DDK-tagged) - Mouse lactate dehydrogenase A (Ldha), transcript variant 2

Product Images

Circular map for MR227451

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ldha
• Synonyms: l7; l7R2; LDH; Ldh-; Ldh1; Ldhm
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227451 representing NM_001136069
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTAAGTCCTCAGGCGGCTACACGTACACGGAGACCTCGGTATTATTTTTCCATTTCAAGGTCTCAA
  AAGATTCAAAGTCCAAGATGGCAACCCTCAAGGACCAGCTGATTGTGAATCTTCTTAAGGAAGAGCAGGC
  TCCCCAGAACAAGATTACAGTTGTTGGGGTTGGTGCTGTTGGCATGGCTTGTGCCATCAGTATCTTAATG
  AAGGACTTGGCGGATGAGCTTGCCCTTGTTGACGTCATGGAAGACAAACTCAAGGGCGAGATGATGGATC
  TCCAGCATGGCAGCCTCTTCCTTAAAACACCAAAAATTGTCTCCAGCAAAGACTACTGTGTAACTGCGAA
  CTCCAAGCTGGTCATTATCACCGCGGGGGCCCGTCAGCAAGAGGGGGAGAGCCGGCTCAACCTGGTCCAG
  CGAAACGTGAACATCTTCAAGTTCATCATTCCCAACATTGTCAAGTACAGTCCACACTGCAAGCTGCTGA
  TCGTCTCCAATCCAGTGGATATCTTGACCTACGTGGCTTGGAAAATCAGTGGCTTTCCCAAAAACCGAGT
  AATTGGAAGTGGTTGCAATCTGGATTCAGCGCGGTTCCGTTACCTGATGGGAGAGAGGCTGGGGGTTCAC
  GCGCTGAGCTGTCACGGCTGGGTCCTGGGAGAACATGGCGACTCCAGTGTGCCTGTGTGGAGTGGTGTGA
  ATGTTGCCGGCGTCTCCCTGAAGTCTCTTAACCCAGAACTGGGCACTGACGCAGACAAGGAGCAGTGGAA
  GGAGGTTCACAAGCAGGTGGTGGACAGTGCCTACGAGGTGATCAAGCTGAAAGGTTACACATCCTGGGCC
  ATTGGCCTCTCTGTGGCAGACTTGGCTGAGAGCATAATGAAGAACCTTAGGCGGGTGCATCCCATTTCCA
  CCATGATTAAGGGTCTCTATGGAATCAATGAGGATGTCTTCCTCAGTGTCCCATGTATCCTGGGACAAAA
  TGGAATCTCGGATGTTGTGAAGGTGACACTGACTCCTGAGGAAGAGGCCCGCCTGAAGAAGAGCGCAGAC
  ACCCTCTGGGGAATCCAGAAGGAGCTGCAGTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227451 representing NM_001136069
  Red=Cloning site Green=Tags(s)
  
  MSKSSGGYTYTETSVLFFHFKVSKDSKSKMATLKDQLIVNLLKEEQAPQNKITVVGVGAVGMACAISILM
  KDLADELALVDVMEDKLKGEMMDLQHGSLFLKTPKIVSSKDYCVTANSKLVIITAGARQQEGESRLNLVQ
  RNVNIFKFIIPNIVKYSPHCKLLIVSNPVDILTYVAWKISGFPKNRVIGSGCNLDSARFRYLMGERLGVH
  ALSCHGWVLGEHGDSSVPVWSGVNVAGVSLKSLNPELGTDADKEQWKEVHKQVVDSAYEVIKLKGYTSWA
  IGLSVADLAESIMKNLRRVHPISTMIKGLYGINEDVFLSVPCILGQNGISDVVKVTLTPEEEARLKKSAD
  TLWGIQKELQF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001136069
• ORF Size: 1083 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001136069.2, NP_001129541.2
• RefSeq Size: 1854 bp
• RefSeq ORF: 1086 bp
• Locus ID: 16828
• Cytogenetics: 7 30.6 cM
• MW: 39.8 kDa
• Gene Summary: The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to hemolytic anemia and early postimplantation death in mice. Multiple transcript variants encoding different isoforms have been found for this gene. The mouse genome contains multiple pseudogenes of this gene. [provided by RefSeq, May 2013]