Ldha (NM_001136069) Mouse Untagged Clone

CAT#: MC208873

Ldha (untagged) - Mouse lactate dehydrogenase A (Ldha), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ldha
• Synonyms: l7; l7R2; LDH; Ldh-; Ldh1; Ldhm
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208873 representing NM_001136069
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTAAGTCCTCAGGCGGCTACACGTACACGGAGACCTCGGTATTATTTTTCCATTTCAAGGTCTCAA
  AAGATTCAAAGTCCAAGATGGCAACCCTCAAGGACCAGCTGATTGTGAATCTTCTTAAGGAAGAGCAGGC
  TCCCCAGAACAAGATTACAGTTGTTGGGGTTGGTGCTGTTGGCATGGCTTGTGCCATCAGTATCTTAATG
  AAGGACTTGGCGGATGAGCTTGCCCTTGTTGACGTCATGGAAGACAAACTCAAGGGCGAGATGATGGATC
  TCCAGCATGGCAGCCTCTTCCTTAAAACACCAAAAATTGTCTCCAGCAAAGACTACTGTGTAACTGCGAA
  CTCCAAGCTGGTCATTATCACCGCGGGGGCCCGTCAGCAAGAGGGGGAGAGCCGGCTCAACCTGGTCCAG
  CGAAACGTGAACATCTTCAAGTTCATCATTCCCAACATTGTCAAGTACAGTCCACACTGCAAGCTGCTGA
  TCGTCTCCAATCCAGTGGATATCTTGACCTACGTGGCTTGGAAAATCAGTGGCTTTCCCAAAAACCGAGT
  AATTGGAAGTGGTTGCAATCTGGATTCAGCGCGGTTCCGTTACCTGATGGGAGAGAGGCTGGGGGTTCAC
  GCGCTGAGCTGTCACGGCTGGGTCCTGGGAGAACATGGCGACTCCAGTGTGCCTGTGTGGAGTGGTGTGA
  ATGTTGCCGGCGTCTCCCTGAAGTCTCTTAACCCAGAACTGGGCACTGACGCAGACAAGGAGCAGTGGAA
  GGAGGTTCACAAGCAGGTGGTGGACAGTGCCTACGAGGTGATCAAGCTGAAAGGTTACACATCCTGGGCC
  ATTGGCCTCTCTGTGGCAGACTTGGCTGAGAGCATAATGAAGAACCTTAGGCGGGTGCATCCCATTTCCA
  CCATGATTAAGGGTCTCTATGGAATCAATGAGGATGTCTTCCTCAGTGTCCCATGTATCCTGGGACAAAA
  TGGAATCTCGGATGTTGTGAAGGTGACACTGACTCCTGAGGAAGAGGCCCGCCTGAAGAAGAGCGCAGAC
  ACCCTCTGGGGAATCCAGAAGGAGCTGCAGTTCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001136069
• Insert Size: 1086 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001136069.2, NP_001129541.2
• RefSeq Size: 1854 bp
• RefSeq ORF: 1086 bp
• Locus ID: 16828
• Cytogenetics: 7 30.6 cM
• Gene Summary: The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to hemolytic anemia and early postimplantation death in mice. Multiple transcript variants encoding different isoforms have been found for this gene. The mouse genome contains multiple pseudogenes of this gene. [provided by RefSeq, May 2013]
  Transcript Variant: This variant (2) uses an alternate 5' exon that results in a distinct 5' UTR and translation initiation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a longer N-terminus compared to isoform 1.