Tfap2b (NM_001025305) Mouse Tagged ORF Clone

CAT#: MR227246

Tfap2b (Myc-DDK-tagged) - Mouse transcription factor AP-2 beta (Tcfap2b), transcript variant 2

Product Images

Circular map for MR227246

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tfap2b
• Synonyms: AI606113; AP-2(beta); E130018K07Rik; Tcfap2b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227246 representing NM_001025305
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTAGTCCACACCTATTCATCCATGGACCGGCACGATGGCGTCCCAAGCCATAGCTCGAGACTCTCTC
  AACTGGGCTCTGTGTCCCAAGGACCCTACTCAAGCGCCCCGCCGCTGTCCCACACGCCATCATCGGACTT
  CCAGCCACCCTACTTTCCGCCCCCCTACCAGCCGCTACCCTACCACCAGAGTCAAGACCCGTACTCCCAC
  GTCAACGATCCCTACTCCCTGAACCCTCTGCATCAGCCCCAGCAGCACCCGTGGGGGCAACGGCAACGCC
  AAGAAGTGGGCTCAGAAGCCGGCTCTCTCCTGCCCCAGCCCCGGGCAGCCTTGCCCCAGCTCTCCGGCCT
  TGATCCCCGAAGGGACTACCACTCTGTCCGCCGGCCGGACGTGCTGCTGCATTCCGCACATCACGGCCTG
  GACGCCGGCATGGGCGACAGCCTCTCGTTGCACGGCCTTGGGCATCCCGGCATGGAAGACGTGCAGTCAG
  TTGAAGATGCCAATAACAGCGGCATGAACCTATTGGACCAGTCAGTCATTAAAAAAGTTCCTGTCCCTCC
  CAAATCTGTGACTTCTCTAATGATGAATAAAGATGGCTTCTTGGGAGGAATGTCAGTCAACACCGGCGAG
  GTATTTTGCTCAGTCCCGGGCCGTTTATCTCTACTCAGTTCAACTTCAAAGTACAAAGTCACTGTGGGCG
  AAGTTCAGAGAAGGCTCTCGCCCCCTGAATGCCTCAATGCATCTCTCCTGGGCGGCGTCCTCAGAAGAGC
  CAAATCGAAAAATGGGGGGAGATCCTTGAGAGAAAGGCTAGAAAAAATCGGTTTGAATTTACCCGCGGGC
  AGGCGCAAAGCAGCAAATGTCACGTTACTCACCTCACTGGTAGAAGGGGAAGCTGTTCACTTAGCTCGGG
  ATTTCGGGTATATTTGTGAAACAGAGTTTCCTGCTAAAGCCGTGTCCGAGTATTTGAACAGACAGCACAC
  GGACCCCAGTGACCTGCACTCCAGAAAGAATATGCTGCTGGCCACCAAGCAACTTTGTAAAGAATTTACG
  GATCTGCTGGCTCAGGACCGGACACCGATCGGAAACAGCAGGCCCAGCCCCATCCTGGAGCCGGGCATCC
  AAAGCTGTCTCACGCACTTCAGTCTCATCACGCACGGCTTCGGTGCCCCGGCCATTTGCGCTGCGCTCAC
  GGCCCTGCAGAACTATCTCACCGAGGCGCTCAAAGGCATGGACAAGATGTTCTTGAACAACACCACTAAC
  AGGCACACGTCTGGGGAAGGCCCAGGTAGTAAAACTGGCGACAAGGAGGAGAAACACAGGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227246 representing NM_001025305
  Red=Cloning site Green=Tags(s)
  
  MLVHTYSSMDRHDGVPSHSSRLSQLGSVSQGPYSSAPPLSHTPSSDFQPPYFPPPYQPLPYHQSQDPYSH
  VNDPYSLNPLHQPQQHPWGQRQRQEVGSEAGSLLPQPRAALPQLSGLDPRRDYHSVRRPDVLLHSAHHGL
  DAGMGDSLSLHGLGHPGMEDVQSVEDANNSGMNLLDQSVIKKVPVPPKSVTSLMMNKDGFLGGMSVNTGE
  VFCSVPGRLSLLSSTSKYKVTVGEVQRRLSPPECLNASLLGGVLRRAKSKNGGRSLRERLEKIGLNLPAG
  RRKAANVTLLTSLVEGEAVHLARDFGYICETEFPAKAVSEYLNRQHTDPSDLHSRKNMLLATKQLCKEFT
  DLLAQDRTPIGNSRPSPILEPGIQSCLTHFSLITHGFGAPAICAALTALQNYLTEALKGMDKMFLNNTTN
  RHTSGEGPGSKTGDKEEKHRK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025305
• ORF Size: 1323 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025305.2, NP_001020476.1
• RefSeq Size: 6056 bp
• RefSeq ORF: 1326 bp
• Locus ID: 21419
• Cytogenetics: 1 A3
• MW: 48.6 kDa
• Gene Summary: Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-beta appears to be required for normal face and limb development and for proper terminal differentiation and function of renal tubular epithelia.[UniProtKB/Swiss-Prot Function]