Tfap2b (NM_001025305) Mouse Untagged Clone

CAT#: MC215809

Tfap2b (untagged) - Mouse transcription factor AP-2 beta (Tcfap2b), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tfap2b
• Synonyms: AI606113; AP-2(beta); E130018K07Rik; Tcfap2b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC215809 representing NM_001025305
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTAGTCCACACCTATTCATCCATGGACCGGCACGATGGCGTCCCAAGCCATAGCTCGAGACTCTCTC
  AACTGGGCTCTGTGTCCCAAGGACCCTACTCAAGCGCCCCGCCGCTGTCCCACACGCCATCATCGGACTT
  CCAGCCACCCTACTTTCCGCCCCCCTACCAGCCGCTACCCTACCACCAGAGTCAAGACCCGTACTCCCAC
  GTCAACGATCCCTACTCCCTGAACCCTCTGCATCAGCCCCAGCAGCACCCGTGGGGGCAACGGCAACGCC
  AAGAAGTGGGCTCAGAAGCCGGCTCTCTCCTGCCCCAGCCCCGGGCAGCCTTGCCCCAGCTCTCCGGCCT
  TGATCCCCGAAGGGACTACCACTCTGTCCGCCGGCCGGACGTGCTGCTGCATTCCGCACATCACGGCCTG
  GACGCCGGCATGGGCGACAGCCTCTCGTTGCACGGCCTTGGGCATCCCGGCATGGAAGACGTGCAGTCAG
  TTGAAGATGCCAATAACAGCGGCATGAACCTATTGGACCAGTCAGTCATTAAAAAAGTTCCTGTCCCTCC
  CAAATCTGTGACTTCTCTAATGATGAATAAAGATGGCTTCTTGGGAGGAATGTCAGTCAACACCGGCGAG
  GTATTTTGCTCAGTCCCGGGCCGTTTATCTCTACTCAGTTCAACTTCAAAGTACAAAGTCACTGTGGGCG
  AAGTTCAGAGAAGGCTCTCGCCCCCTGAATGCCTCAATGCATCTCTCCTGGGCGGCGTCCTCAGAAGAGC
  CAAATCGAAAAATGGGGGGAGATCCTTGAGAGAAAGGCTAGAAAAAATCGGTTTGAATTTACCCGCGGGC
  AGGCGCAAAGCAGCAAATGTCACGTTACTCACCTCACTGGTAGAAGGGGAAGCTGTTCACTTAGCTCGGG
  ATTTCGGGTATATTTGTGAAACAGAGTTTCCTGCTAAAGCCGTGTCCGAGTATTTGAACAGACAGCACAC
  GGACCCCAGTGACCTGCACTCCAGAAAGAATATGCTGCTGGCCACCAAGCAACTTTGTAAAGAATTTACG
  GATCTGCTGGCTCAGGACCGGACACCGATCGGAAACAGCAGGCCCAGCCCCATCCTGGAGCCGGGCATCC
  AAAGCTGTCTCACGCACTTCAGTCTCATCACGCACGGCTTCGGTGCCCCGGCCATTTGCGCTGCGCTCAC
  GGCCCTGCAGAACTATCTCACCGAGGCGCTCAAAGGCATGGACAAGATGTTCTTGAACAACACCACTAAC
  AGGCACACGTCTGGGGAAGGCCCAGGTAGTAAAACTGGCGACAAGGAGGAGAAACACAGGAAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001025305
• Insert Size: 1326 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025305.2, NP_001020476.1
• RefSeq Size: 6167 bp
• RefSeq ORF: 1326 bp
• Locus ID: 21419
• Cytogenetics: 1 A3
• Gene Summary: Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-beta appears to be required for normal face and limb development and for proper terminal differentiation and function of renal tubular epithelia.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) contains an alternate exon in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon compared to variant 1. The encoded protein (isoform 2) is shorter and has a distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.