Pik3cg (NM_001146200) Mouse Tagged ORF Clone

CAT#: MR227194

Pik3cg (Myc-DDK-tagged) - Mouse phosphoinositide-3-kinase, catalytic, gamma polypeptide (Pik3cg), transcript variant 3

Product Images

Circular map for MR227194

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pik3cg
• Synonyms: 5830428L06Rik; p110gamma; PI3Kgamma
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227194 representing NM_001146200
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTGGAGAACTATGAACAACCGGTGGTTCTAAGAGAGGACAACCTCCGCCGGCGCCGGAGGATGA
  AGCCACGCAGCGCAGCAGGCAGCCTGTCTTCCATGGAGCTCATCCCCATTGAGTTCGTACTGCCCACCAG
  CCAGCGCATCAGCAAGACTCCAGAAACAGCGCTGCTGCATGTGGCTGGCCATGGCAATGTGGAACAGATG
  AAAGCTCAGGTGTGGCTGCGCGCACTGGAGACCAGTGTGGCTGCGGAGTTCTACCACCGATTGGGCCCGG
  ACCAATTCCTCCTGCTCTACCAGAAGAAAGGACAATGGTATGAGATCTATGACAGGTACCAAGTGGTGCA
  GACCCTAGACTGCCTGCATTACTGGAAGTTGATGCACAAGAGCCCTGGCCAGATCCACGTGGTACAGCGA
  CACGTACCTTCTGAGGAGACCTTGGCTTTCCAGAAGCAGCTCACCTCCCTGATTGGCTATGACGTCACTG
  ACATCAGCAATGTGCACGATGATGAGCTAGAGTTCACTCGCCGCCGTCTGGTTACGCCCCGCATGGCTGA
  AGTGGCTGGCCGGGATGCCAAACTCTATGCTATGCACCCTTGGGTAACGTCCAAACCTCTCCCAGACTAC
  CTGTCAAAAAAGATTGCCAACAACTGCATCTTCATCGTCATCCACCGCGGTACCACCAGCCAAACCATCA
  AGGTCTCCGCAGATGATACTCCTGGTACCATCCTCCAGAGCTTCTTCACCAAGATGGCCAAGAAGAAGTC
  CCTAATGAATATCTCAGAAAGTCAAAGTGAGCAGGATTTTGTATTGCGGGTTTGTGGCCGCGATGAGTAC
  CTGGTGGGTGAAACACCCCTCAAAAATTTCCAGTGGGTGAGGCAGTGCCTCAAGAACGGAGATGAAATAC
  ACCTGGTGCTCGACACGCCTCCAGACCCAGCCCTTGATGAGGTGAGGAAGGAAGAATGGCCGCTGGTGGA
  TGACTGCACTGGAGTCACCGGCTACCACGAGCAGCTGACCATCCATGGCAAGGACCACGAGAGTGTGTTC
  ACAGTGTCTTTGTGGGACTGCGACCGAAAGTTCAGGGTCAAGATCAGAGGCATTGATATCCCTGTCCTGC
  CTCGGAACACCGACCTCACTGTGTTTGTGGAAGCGAACATCCAGCACGGGCAACAAGTCCTCTGCCAAAG
  GAGAACCAGCCCTAAGCCCTTCGCAGAAGAGGTACTCTGGAATGTGTGGCTGGAGTTTGGCATCAAAATC
  AAAGACTTGCCCAAAGGGGCTCTATTGAACCTACAGATCTACTGCTGCAAAACCCCATCACTGTCCAGCA
  AGGCTTCTGCAGAGACTCCAGGCTCCGAGTCCAAGGGCAAAGCCCAGCTTCTCTATTACGTGAACTTGCT
  GTTAATAGACCACCGTTTCCTCCTCCGCCACGGGGACTATGTGCTCCACATGTGGCAGATATCTGGCAAG
  GCAGAGGAGCAGGGCAGCTTCAATGCTGACAAGCTCACATCCGCAACCAATCCTGACAAGGAGAACTCAA
  TGTCCATTTCCATCCTGCTGGACAATTACTGTCACCCCATAGCTTTGCCTAAGCACCGGCCCACCCCTGA
  CCCAGAGGGAGACAGGGTTCGGGCTGAAATGCCCAATCAGCTTCGAAAGCAATTGGAGGCGATCATAGCC
  ACAGATCCACTTAACCCCCTCACAGCAGAGGACAAAGAATTGCTCTGGCATTTTCGATATGAAAGCCTGA
  AGCATCCGAAGGCTTACCCTAAGCTATTCAGCTCAGTGAAATGGGGGCAGCAAGAAATTGTTGCCAAAAC
  GTACCAGCTGTTAGCCAGAAGGGAGATCTGGGATCAAAGTGCTTTGGACGTTGGCTTAACCATGCAGCTC
  CTGGACTGCAACTTTTCAGACGAGAATGTCCGGGCCATTGCAGTTCAGAAACTGGAGAGCTTAGAGGACG
  ATGACGTTTTACATTACCTTCTCCAGCTGGTACAGGCTGTGAAATTTGAACCGTACCACGACAGTGCGCT
  GGCCAGATTCCTGCTGAAGCGTGGCTTGAGGAACAAAAGAATCGGTCACTTCTTGTTCTGGTTCCTGCGA
  AGTGAGATCGCACAGTCCAGACACTATCAGCAGAGGTTCGCTGTGATCCTGGAGGCGTACCTGCGAGGCT
  GTGGCACAGCCATGTTGCAGGACTTCACACAGCAGGTCCATGTGATTGAGATGTTACAGAAAGTCACCAT
  TGATATTAAATCGCTCTCGGCAGAGAAGTATGACGTCAGTTCCCAAGTTATTTCACAGCTTAAGCAAAAG
  CTTGAAAGCCTTCAGAACTCCAATCTCCCCGAGAGCTTTAGAGTTCCCTATGATCCTGGACTAAAAGCCG
  GTACCCTGGTGATCGAGAAATGCAAAGTGATGGCCTCCAAGAAGAAGCCCCTGTGGCTTGAGTTTAAGTG
  TGCTGATCCCACAGTCCTATCCAACGAAACCATTGGAATCATCTTTAAACATGGTGATGATCTGCGCCAA
  GACATGTTGATCTTGCAGATTCTACGCATCATGGAGTCCATTTGGGAGACTGAATCTCTGGACCTGTGCC
  TTCTGCCTTACGGTTGCATCTCAACTGGTGACAAAATAGGAATGATCGAGATTGTAAAGGATGCCACAAC
  GATCGCTCAAATTCAGCAAAGCACAGTGGGTAACACGGGGGCATTCAAAGATGAAGTCCTGAATCACTGG
  CTCAAGGAAAAATGTCCTATTGAAGAAAAGTTTCAGGCCGCAGTGGAAAGGTTTGTTTACTCCTGTGCAG
  GCTACTGTGTGGCCACATTTGTTCTTGGGATCGGTGACAGGCACAACGACAACATTATGATCTCAGAGAC
  AGGAAACCTATTTCATATAGACTTCGGACACATTCTTGGGAATTACAAGAGTTTCCTGGGCATCAATAAA
  GAGAGAGTGCCCTTCGTCCTAACCCCAGACTTCTTGTTTGTGATGGGATCTTCTGGAAAAAAGACAAGTC
  CACACTTCCAGAAATTCCAGGATGTCTGTGTTAGAGCTTACCTAGCTCTTCGCCATCACACAAACCTGTT
  GATCATCTTGTTCTCCATGATGCTGATGACAGGAATGCCCCAGCTGACAAGCAAAGAGGACATTGAATAT
  ATCCGGGATGCCCTCACCGTGGGAAAAAGCGAGGAGGACGCTAAGAAATATTTCCTTGATCAGATCGAAG
  TCTGCAGAGACAAAGGATGGACTGTGCAGTTTAACTGGTTCCTACATCTTGTTCTTGGCATCAAACAAGG
  AGAAAAGCACTCCGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227194 representing NM_001146200
  Red=Cloning site Green=Tags(s)
  
  MELENYEQPVVLREDNLRRRRRMKPRSAAGSLSSMELIPIEFVLPTSQRISKTPETALLHVAGHGNVEQM
  KAQVWLRALETSVAAEFYHRLGPDQFLLLYQKKGQWYEIYDRYQVVQTLDCLHYWKLMHKSPGQIHVVQR
  HVPSEETLAFQKQLTSLIGYDVTDISNVHDDELEFTRRRLVTPRMAEVAGRDAKLYAMHPWVTSKPLPDY
  LSKKIANNCIFIVIHRGTTSQTIKVSADDTPGTILQSFFTKMAKKKSLMNISESQSEQDFVLRVCGRDEY
  LVGETPLKNFQWVRQCLKNGDEIHLVLDTPPDPALDEVRKEEWPLVDDCTGVTGYHEQLTIHGKDHESVF
  TVSLWDCDRKFRVKIRGIDIPVLPRNTDLTVFVEANIQHGQQVLCQRRTSPKPFAEEVLWNVWLEFGIKI
  KDLPKGALLNLQIYCCKTPSLSSKASAETPGSESKGKAQLLYYVNLLLIDHRFLLRHGDYVLHMWQISGK
  AEEQGSFNADKLTSATNPDKENSMSISILLDNYCHPIALPKHRPTPDPEGDRVRAEMPNQLRKQLEAIIA
  TDPLNPLTAEDKELLWHFRYESLKHPKAYPKLFSSVKWGQQEIVAKTYQLLARREIWDQSALDVGLTMQL
  LDCNFSDENVRAIAVQKLESLEDDDVLHYLLQLVQAVKFEPYHDSALARFLLKRGLRNKRIGHFLFWFLR
  SEIAQSRHYQQRFAVILEAYLRGCGTAMLQDFTQQVHVIEMLQKVTIDIKSLSAEKYDVSSQVISQLKQK
  LESLQNSNLPESFRVPYDPGLKAGTLVIEKCKVMASKKKPLWLEFKCADPTVLSNETIGIIFKHGDDLRQ
  DMLILQILRIMESIWETESLDLCLLPYGCISTGDKIGMIEIVKDATTIAQIQQSTVGNTGAFKDEVLNHW
  LKEKCPIEEKFQAAVERFVYSCAGYCVATFVLGIGDRHNDNIMISETGNLFHIDFGHILGNYKSFLGINK
  ERVPFVLTPDFLFVMGSSGKKTSPHFQKFQDVCVRAYLALRHHTNLLIILFSMMLMTGMPQLTSKEDIEY
  IRDALTVGKSEEDAKKYFLDQIEVCRDKGWTVQFNWFLHLVLGIKQGEKHSA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001146200
• ORF Size: 3306 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001146200.2
• RefSeq Size: 6634 bp
• RefSeq ORF: 3309 bp
• Locus ID: 30955
• UniProt ID: Q9JHG7
• Cytogenetics: 12 A3
• MW: 126.4 kDa
• Gene Summary: Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Links G-protein coupled receptor activation to PIP3 production. Involved in immune, inflammatory and allergic responses. Modulates leukocyte chemotaxis to inflammatory sites and in response to chemoattractant agents. May control leukocyte polarization and migration by regulating the spatial accumulation of PIP3 and by regulating the organization of F-actin formation and integrin-based adhesion at the leading edge. Controls motility of dendritic cells. Together with PIK3CD is involved in natural killer (NK) cell development and migration towards the sites of inflammation. Participates in T-lymphocyte migration. Regulates T-lymphocyte proliferation and cytokine production. Together with PIK3CD participates in T-lymphocyte development. Required for B-lymphocyte development and signaling. Together with PIK3CD participates in neutrophil respiratory burst. Together with PIK3CD is involved in neutrophil chemotaxis and extravasation. Together with PIK3CB promotes platelet aggregation and thrombosis. Regulates alpha-IIb/beta-3 integrins (ITGA2B/ ITGB3) adhesive function in platelets downstream of P2Y12 through a lipid kinase activity-independent mechanism. May have also a lipid kinase activity-dependent function in platelet aggregation. Involved in endothelial progenitor cell migration. Negative regulator of cardiac contractility. Modulates cardiac contractility by anchoring protein kinase A (PKA) and PDE3B activation, reducing cAMP levels. Regulates cardiac contractility also by promoting beta-adrenergic receptor internalization by binding to GRK2 and by non-muscle tropomyosin phosphorylation. Also has serine/threonine protein kinase activity: both lipid and protein kinase activities are required for beta-adrenergic receptor endocytosis. May also have a scaffolding role in modulating cardiac contractility. Contribute to cardiac hypertrophy under pathological stress. Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which the PI3K gamma complex is activated by RAPGEF3 and which is involved in angiogenesis (By similarity).[UniProtKB/Swiss-Prot Function]