Trp53bp1 (NM_013735) Mouse Tagged ORF Clone

CAT#: MR226520

Trp53bp1 (Myc-DDK-tagged) - Mouse transformation related protein 53 binding protein 1 (Trp53bp1)

Product Images

Circular map for MR226520

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Trp53bp1
• Synonyms: 53BP1; m53BP1; p53BP1; Tp53bp1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226520 representing NM_013735
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGGGGAGCAGATGGACCCTACTGGAAGTCAATTGGATTCAGATTTCTCTCAGCAAGACACTCCTT
  GCCTGATAATAGAAGATTCTCAGCCTGAAAGCCAGGTTCTGGAAGAAGATGCAGGCTCTCACTTCAGCGT
  GCTATCTCGACACCTTCCTAATCTGCAGATGCACAAAGAGAACCCCGTGTTGGATATTGTATCAAATCCG
  GAACAATCTGCTGTAGAACAAGGAGACAGTAATAGCTCATTCAATGAACATCTGAAAGAAAAGAAAGCTT
  CAGATCCTGTGGAGTCTTCTCATTTGGGTACCAGTGGTTCCATCAGTCAGGTCATTGAACGGTTACCTCA
  GCCAAACAGGACAAGCAGTGCTCTGGCAGTGACAGTAGAAGCTGCTTCTCTCCCAGAGGAGGAGAAGGAA
  GAAGAGGAGTTAGAGGAGGAGAAGGAAGGGGTGGGAGCAAACGCTCCCGGTGCTGACTCCCTTGCTGCTG
  AAGATTCTGCTTCATCACAGTTGGGCTTTGGAGTTCTGGAACTGTCCCAGAGCCAGGATGTTGAAGAACA
  TACAGTGCCATATGATGTCAACCAAGAGCATCTGCAGTTGGTGACCACTAACTCGGGTTCTAGCCCGCTA
  TCTGATGTGGATGCGAGCACTGCAATTAAATGTGAAGAACAGCCCACTGAAGATATTGCCATGATAGAAC
  AGCCCAGCAAAGACATCCCTGTTACAGTACAGCCCGGTAAAGGTATCCATGTGGTAGAAGAACAAAATCT
  ACCACTTGTAAGGTCCGAAGACCGGCCGTCCAGTCCTCAAGTTTCTGTTGCTGCTGTGGAAACAAAGGAA
  CAGGTACCTGCCCGGGAGCTGCTGGAAGAGGGGCCGCAGGTTCAGCCGTCATCAGAGCCTGAGGTTTCCT
  CAACCCAGGAGGACTTGTTTGACCAGAGTAGTAAAACAGCTTCTGATGGTTGTTCTACTCCTTCAAGGGA
  GGAAGGTGGGTGCTCTCCGGTTTCCACACCTGCTACCACCCTGCAGCTCCTGCAGCTCTCTGGTCAGAAG
  CCCCTTGTTCAGGAGAGTCTTTCCACGAATTCCTCAGATCTTGTTGCTCCTTCCCCTGATGCTTTCCGAT
  CTACCCCTTTTATCGTTCCTAGCAGTCCCACAGAGCAAGGAGGGAGAAAAGATGAGCCCATGGATATGTC
  AGTGATACCTGTAGGAGGGGAGCCTTTCCAGAAGCTTCATGATGACGAAGCAATGGAGACAGAAAAACCC
  CTTCTCCCGTCTCAGCCTGCTGTGTCACCGCAAGCATCAACACCAGTGTCTCGGAGCACGCCAGTTTTCA
  CTCCTGGCTCTCTTCCCATCCCGTCCCAGCCCGAGTTCTCTCATGACATTTTCATTCCATCACCAAGTCT
  GGAAGAACCATCAGATGATGTGAAGAAAGGTGGAGGTTTACATAGCTCATCTCTTACTGTTGAGTGTTCC
  AAGACTTCAGAGAGTGAACCAAAGAATTTCACTGATGACCTTGGGCTCTCCATGACAGGGGATTCTTGCA
  AACTGATGCTTTCTACAAGTGAGTATAGTCAGTCCTCAAAGATGGAGAGCTTGGGTTCTCCCAGGACTGA
  GGAAGACAGAGAGAACACACAGATTGACGATACTGAACCTTTGTCTCCAGTTAGCAATTCTAAACTTCCT
  GCTGACAGTGAGAATGTCCTGGTGACTCCATCGCAGGACGACCAGGTAGAAATGAGTCAGAATGTAGATA
  AAGCAAAAGAGGATGAAACGGAGGACAGAGGTGACTGTAAAGGCAGAGAAGACGCGGTTGCTGAAGATGT
  TTGCATCGACCTCACTTGTGATTCTGGGAGTCAGGCAGTTCCTTCTCCAGCTACCCGTTCGGAGGCACTT
  TCCAGTGTCTTAGATCAGGAGGAAGCTATGGACACTAAAGAACACCATCCAGAGGAAGGGTTTTCGGGAT
  CTGAAGTAGAAGAAGTCCCTGAGACTCCCTGTGGAAGTCACAGAGAGGAGCCCAAAGAAGAACCGATGGA
  AAGTATCCCACTGCACCTTTCTCTGACTGAAACCCAGTCTGAGGCATTGTGTCTGCAGAAGGAAGCCCCC
  AAAGAGGAATGCCCAGAAGCTATGGAAGTTGAAACCAGTGTGATTAGTATTGACTCCCCCCAGAAACTGC
  AAGTACTTGACCAGGAATTAGAGCATAAGGACCCAGACACCTGGGAAGAAGCTACTTCTGAGGACTCCAG
  TGTTGTTATCGTTGATGTGAAGGAGCCCTCTCCTCGAGCTGATGTTTCCTGTGAACCTTTGGAGGAGGTG
  GAGAAATGCTCTGACTCCCAGTCCTGGGAGGGTGTGGCTCCAGAGGAAGAACCATGTGCTGAGAATAGAT
  TAGATACTCCAGAAGAAAAGCGTATAGAATGTGATGGAGATTCAAAAGCAGAGACCACAGAAAAGGACGC
  TGTAACAGAAGACTCTCCACAACCTCCTTTGCCTTCAGTGAGAGACGAACCTGTCAGACCCGATCAGGAG
  ACACAGCAGCCCCAAGTTCAAGAGAAAGAGAGCCCAGTGACTGTAGATGCAGAAGTGGCTGATGACAAGC
  AGCTGGGACCAGAGGGCGCATGCCAGCAGCTTGAGAAGGCCCCTGCCTGTGCCTCACAGAGTTTCTGTGA
  AAGTTCTAGTGAAACTCCATTTCATTTCACTTTGCCTAAAGAAGGTGATATTATCCCACCATTGACTGGC
  GCAACCCCACCTCTTATTGGGCACCTAAAATTGGAGCCCAAGAGACATAGTACTCCTATTGGGATTAGCA
  ACTATCCAGAAAGCACCATAGCAACCAGTGATGTCACATCTGAAAGCATGGTGGAGATCAATGATCCTCT
  ACTTGGGAATGAAAAAGGGGATTCTGAGTCTGCCCCAGAAATGGATGGAAAACTGTCTCTGAAAATGAAA
  CTGGTTAGTCCTGAGACAGAGGCCAGTGAAGAATCTTTGCAATTTAGCCTGGAAAAGCCTACAACTGCTG
  AGAGAAAAAATGGATCTACTGCCATTGCAGAGCCTGTTGCCAGTCTCCAGAAGCCGGTGCCTGTGTTTGG
  CTGCATCTATGAAGCCCAGCAAGAGAAGGAGGCTCAAAGTGAGGCTCCTCCCTCTGCACCCGACAGGGCG
  AATTTGCTCCATTTTCCAAGTGCTCAAGAAGAAGACAAAGAAAGACCAGATGTTACCCCAAAACTCAGGC
  AGAGTGAACAGCCTGTGAAGCCTGTTGGGCCTGTCATGGACGATGCTGCTCCTGAGGACTCAGCTTCTCC
  TGTTTCGCAGCAGAGGGCCTCACAGGAGCAGAGGGCCTCACAGGAGCCCTTCAGTCCTGCAGAGGACGTG
  ATGGAAACAGACCTGCTGGAAGGACTGGCTGCTAACCAGGACAGACCTAGTAAGATGTTGATGGACAGGC
  CCACCCAGAGTAACATCGGGATCCAGACCGTGGACCATTCCCTGTGCGCCCCAGAAACTGTCTCGGCAGC
  AACTCAGACTGTGAAGAGTGTGTGTGAGCAGGGGACCAGTACAGCGGAGCAGAACTCTGGGAAACAAGAT
  GCCACTGTGCAGACTGAGAGGGGGAGTGGAGAGAAACCTGCCAGTGCTCCCGTGGACGACACAGAGTCCC
  TCCACAGCCAGGGGGAAGAGGAATTTGAAATGCCCCAGCCTCCGCATGGCCATGTCTTGCATCGCCACAT
  GAGAACCATTCGAGAAGTCCGTACACTCGTCACCCGCGTCATCACAGATGTTTATTATGTGGATGGGACA
  GAAGTGGAAAGGAAAGTAACGGAGGAGACTGAAGAACCAATCGTAGAGTGTCAGGAATGTGAAACAGAAG
  TTTCCCCTTCACAGACTGGAGGTTCCTCTGGGGACCTGGGGGACATCAGCTCCTTCTCCTCCAAAGCTTC
  CAGCTCACACCACACATCCAGTGGAACAAGTCTCTCAGCCATACACAGCAGTGGCAGCTCAGGGCGAGGA
  GCTGGGCCACTCAAAGGAAAAGCCAGTGGGACAGAAGCTGCAGATTTTGCCTTACCCAGTTCCCGAGGAG
  GCCCAGGAAAACTGAGTCCTAGAAAAGGGATCAGTCAGACAGGGGCACCGGTGTGTGAGGAAGATGGTGA
  TGCAGGCCTTGGCATCAGACAGGGAGGGAAGGCTCCAGTTACACCTCGTGGGCGTGGGCGAAGGGGCCGC
  CCACCTTCTCGGACCACTGGAACCAGAGAAACAGTTGTCTCTGGTCCGTTGGGCGTAGAAGATATTTCAC
  CTAGCATGTCACCAGATGACAAGTCCTTCACCCGCATTATGCCTCGTGTGCCAGATTCTACCAAACGGAC
  GGATGCCAGTTCTAGTACTTTGCGGCGGAGTGATTCTCCAGAGATTCCTTTTCAGGCTGCTACTGGTTCC
  TCTGATGGCTTGGATTCCTCATCTTCAGGAAACAGCTTTGTGGGTCTCCGTGTTGTAGCTAAGTGGTCAT
  CCAATGGCTACTTTTACTCTGGGAAGATCACCCGAGATGTGGGGGCTGGGAAGTACAAGCTGCTCTTTGA
  TGATGGGTACGAATGTGACGTGCTGGGCAAAGACATTCTCCTGTGTGACCCCATCCCCCTGGACACTGAA
  GTGACAGCCCTCTCAGAAGATGAATATTTCAGTGCAGGAGTGGTGAAAGGACATAGGAAGGAGTCTGGGG
  AGCTGTATTACAGCATTGAAAAAGAAGGCCAAAGGAAGTGGTATAAACGAATGGCAGTCATTCTGTCCTT
  GGAGCAAGGAAACAGACTGAGAGAGCAATATGGGCTTGGCCCATATGAAGCTGTCACACCGCTCACGAAG
  GCAGCAGATATCAGCTTAGACAATTTGGTGGAAGGAAAGCGGAAACGGCGCAGTAACATCAGCTCCCCAG
  TCACCCCCACTGCCGCCAGTAGCAGCAGCACAACGCCCACACGTAAAGCCACGGAGAGTCCCCGTGCTTC
  CACGGGAGTCCCATCAGGCAAAAGGAAACTTCCGACTTCTGAAGAGGAACGCTCTCCTGCTAAGCGAGGT
  CGAAAGTCTGCCACCGTGAAACCTGGAACAGTGGGGGCAGCAGAGTTTGTGAGTCCCTGTGAGACTGGAG
  ACAACATAGGTGAGCCTTCTGTCCTAGAAGAGCCAAGAGGGCCTTTGCCCCTCAACAAGACCTTGTTTCT
  GGGCTATGCCTTTCTCCTCACCATGGCTACAACTAGTGACAAGCTGGCCAGTCGCTCTAAGCTGCTAGAT
  GGTCCTACAGGAAGCAGTGAAGAAGAGGAGGAATTTTTAGAAATTCCTCCTTTCAACAAGCAGTATACAG
  AATGCCAGCTTCGAGCAGGAGCTGGGTATATCCTTGAAGACTTCAATGAAGCCCAGTGTAACACAGCCTA
  CCAGTGTCTCCTAATTGCGGACCAGCACTGTCGAACCCGGAAGTACTTCCTGTGCCTTGCCAGTGGCATT
  CCTTGTGTGTCTCATGTCTGGGTCCATGACAGTTGCCATGCCAACCAACTTCAAAACTATCGTAATTATC
  TGCTGCCTGCTGGGTATAGCCTTGAAGAGCAACGAATTCTGGATTGGCAACCCCGTGAAAACCCTTTCCA
  GAATCTGAAGGTCCTCTTGGTGTCAGATCAACAACAGAACTTCTTGGAGCTCTGGTCTGAGATCCTTATG
  ACTGGAGGGGCAGCCTCTGTGAAGCAGCACCATTCAAGTGCCCACAACAAAGACATTGCTTTAGGGGTAT
  TTGATGTGGTGGTGACAGACCCCTCATGCCCAGCCTCGGTGCTCAAGTGTGCTGAAGCCTTGCAACTGCC
  TGTGGTATCACAAGAATGGGTGATCCAGTGCCTCATTGTTGGGGAGAGAATTGGATTCAAGCAGCATCCA
  AAATATAAACATGATTATGTTTCTCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226520 representing NM_013735
  Red=Cloning site Green=Tags(s)
  
  MPGEQMDPTGSQLDSDFSQQDTPCLIIEDSQPESQVLEEDAGSHFSVLSRHLPNLQMHKENPVLDIVSNP
  EQSAVEQGDSNSSFNEHLKEKKASDPVESSHLGTSGSISQVIERLPQPNRTSSALAVTVEAASLPEEEKE
  EEELEEEKEGVGANAPGADSLAAEDSASSQLGFGVLELSQSQDVEEHTVPYDVNQEHLQLVTTNSGSSPL
  SDVDASTAIKCEEQPTEDIAMIEQPSKDIPVTVQPGKGIHVVEEQNLPLVRSEDRPSSPQVSVAAVETKE
  QVPARELLEEGPQVQPSSEPEVSSTQEDLFDQSSKTASDGCSTPSREEGGCSPVSTPATTLQLLQLSGQK
  PLVQESLSTNSSDLVAPSPDAFRSTPFIVPSSPTEQGGRKDEPMDMSVIPVGGEPFQKLHDDEAMETEKP
  LLPSQPAVSPQASTPVSRSTPVFTPGSLPIPSQPEFSHDIFIPSPSLEEPSDDVKKGGGLHSSSLTVECS
  KTSESEPKNFTDDLGLSMTGDSCKLMLSTSEYSQSSKMESLGSPRTEEDRENTQIDDTEPLSPVSNSKLP
  ADSENVLVTPSQDDQVEMSQNVDKAKEDETEDRGDCKGREDAVAEDVCIDLTCDSGSQAVPSPATRSEAL
  SSVLDQEEAMDTKEHHPEEGFSGSEVEEVPETPCGSHREEPKEEPMESIPLHLSLTETQSEALCLQKEAP
  KEECPEAMEVETSVISIDSPQKLQVLDQELEHKDPDTWEEATSEDSSVVIVDVKEPSPRADVSCEPLEEV
  EKCSDSQSWEGVAPEEEPCAENRLDTPEEKRIECDGDSKAETTEKDAVTEDSPQPPLPSVRDEPVRPDQE
  TQQPQVQEKESPVTVDAEVADDKQLGPEGACQQLEKAPACASQSFCESSSETPFHFTLPKEGDIIPPLTG
  ATPPLIGHLKLEPKRHSTPIGISNYPESTIATSDVTSESMVEINDPLLGNEKGDSESAPEMDGKLSLKMK
  LVSPETEASEESLQFSLEKPTTAERKNGSTAIAEPVASLQKPVPVFGCIYEAQQEKEAQSEAPPSAPDRA
  NLLHFPSAQEEDKERPDVTPKLRQSEQPVKPVGPVMDDAAPEDSASPVSQQRASQEQRASQEPFSPAEDV
  METDLLEGLAANQDRPSKMLMDRPTQSNIGIQTVDHSLCAPETVSAATQTVKSVCEQGTSTAEQNSGKQD
  ATVQTERGSGEKPASAPVDDTESLHSQGEEEFEMPQPPHGHVLHRHMRTIREVRTLVTRVITDVYYVDGT
  EVERKVTEETEEPIVECQECETEVSPSQTGGSSGDLGDISSFSSKASSSHHTSSGTSLSAIHSSGSSGRG
  AGPLKGKASGTEAADFALPSSRGGPGKLSPRKGISQTGAPVCEEDGDAGLGIRQGGKAPVTPRGRGRRGR
  PPSRTTGTRETVVSGPLGVEDISPSMSPDDKSFTRIMPRVPDSTKRTDASSSTLRRSDSPEIPFQAATGS
  SDGLDSSSSGNSFVGLRVVAKWSSNGYFYSGKITRDVGAGKYKLLFDDGYECDVLGKDILLCDPIPLDTE
  VTALSEDEYFSAGVVKGHRKESGELYYSIEKEGQRKWYKRMAVILSLEQGNRLREQYGLGPYEAVTPLTK
  AADISLDNLVEGKRKRRSNISSPVTPTAASSSSTTPTRKATESPRASTGVPSGKRKLPTSEEERSPAKRG
  RKSATVKPGTVGAAEFVSPCETGDNIGEPSVLEEPRGPLPLNKTLFLGYAFLLTMATTSDKLASRSKLLD
  GPTGSSEEEEEFLEIPPFNKQYTECQLRAGAGYILEDFNEAQCNTAYQCLLIADQHCRTRKYFLCLASGI
  PCVSHVWVHDSCHANQLQNYRNYLLPAGYSLEEQRILDWQPRENPFQNLKVLLVSDQQQNFLELWSEILM
  TGGAASVKQHHSSAHNKDIALGVFDVVVTDPSCPASVLKCAEALQLPVVSQEWVIQCLIVGERIGFKQHP
  KYKHDYVSH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013735
• ORF Size: 5907 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013735.4, NP_038763.3
• RefSeq Size: 7722 bp
• RefSeq ORF: 5910 bp
• Locus ID: 27223
• UniProt ID: P70399
• Cytogenetics: 2 E5
• MW: 213.2 kDa
• Gene Summary: Double-strand break (DSB) repair protein involved in response to DNA damage, telomere dynamics and class-switch recombination (CSR) during antibody genesis (PubMed:15159415, PubMed:15077110, PubMed:20453858, PubMed:23333305, PubMed:26308889, PubMed:20362325). Plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs and specifically counteracting the function of the homologous recombination (HR) repair protein BRCA1 (PubMed:23333305, PubMed:20362325). In response to DSBs, phosphorylation by ATM promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites. Recruited to DSBs sites by recognizing and binding histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites. Required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs (PubMed:15159415, PubMed:15077110). Participates to the repair and the orientation of the broken DNA ends during CSR (PubMed:26308889). In contrast, it is not required for classic NHEJ and V(D)J recombination (PubMed:15159415). Promotes NHEJ of dysfunctional telomeres (By similarity).[UniProtKB/Swiss-Prot Function]