Trp53bp1 (NM_013735) Mouse Untagged Clone

CAT#: MC225093

Trp53bp1 (untagged) - Mouse transformation related protein 53 binding protein 1 (Trp53bp1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trp53bp1
• Synonyms: 53BP1; m53BP1; p53BP1; Tp53bp1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225093 representing NM_013735
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGGGGAGCAGATGGACCCTACTGGAAGTCAATTGGATTCAGATTTCTCTCAGCAAGACACTCCTT
  GCCTGATAATAGAAGATTCTCAGCCTGAAAGCCAGGTTCTGGAAGAAGATGCAGGCTCTCACTTCAGCGT
  GCTATCTCGACACCTTCCTAATCTGCAGATGCACAAAGAGAACCCCGTGTTGGATATTGTATCAAATCCG
  GAACAATCTGCTGTAGAACAAGGAGACAGTAATAGCTCATTCAATGAACATCTGAAAGAAAAGAAAGCTT
  CAGATCCTGTGGAGTCTTCTCATTTGGGTACCAGTGGTTCCATCAGTCAGGTCATTGAACGGTTACCTCA
  GCCAAACAGGACAAGCAGTGCTCTGGCAGTGACAGTAGAAGCTGCTTCTCTCCCAGAGGAGGAGAAGGAA
  GAAGAGGAGTTAGAGGAGGAGAAGGAAGGGGTGGGAGCAAACGCTCCCGGTGCTGACTCCCTTGCTGCTG
  AAGATTCTGCTTCATCACAGTTGGGCTTTGGAGTTCTGGAACTGTCCCAGAGCCAGGATGTTGAAGAACA
  TACAGTGCCATATGATGTCAACCAAGAGCATCTGCAGTTGGTGACCACTAACTCGGGTTCTAGCCCGCTA
  TCTGATGTGGATGCGAGCACTGCAATTAAATGTGAAGAACAGCCCACTGAAGATATTGCCATGATAGAAC
  AGCCCAGCAAAGACATCCCTGTTACAGTACAGCCCGGTAAAGGTATCCATGTGGTAGAAGAACAAAATCT
  ACCACTTGTAAGGTCCGAAGACCGGCCGTCCAGTCCTCAAGTTTCTGTTGCTGCTGTGGAAACAAAGGAA
  CAGGTACCTGCCCGGGAGCTGCTGGAAGAGGGGCCGCAGGTTCAGCCGTCATCAGAGCCTGAGGTTTCCT
  CAACCCAGGAGGACTTGTTTGACCAGAGTAGTAAAACAGCTTCTGATGGTTGTTCTACTCCTTCAAGGGA
  GGAAGGTGGGTGCTCTCCGGTTTCCACACCTGCTACCACCCTGCAGCTCCTGCAGCTCTCTGGTCAGAAG
  CCCCTTGTTCAGGAGAGTCTTTCCACGAATTCCTCAGATCTTGTTGCTCCTTCCCCTGATGCTTTCCGAT
  CTACCCCTTTTATCGTTCCTAGCAGTCCCACAGAGCAAGGAGGGAGAAAAGATGAGCCCATGGATATGTC
  AGTGATACCTGTAGGAGGGGAGCCTTTCCAGAAGCTTCATGATGACGAAGCAATGGAGACAGAAAAACCC
  CTTCTCCCGTCTCAGCCTGCTGTGTCACCGCAAGCATCAACACCAGTGTCTCGGAGCACGCCAGTTTTCA
  CTCCTGGCTCTCTTCCCATCCCGTCCCAGCCCGAGTTCTCTCATGACATTTTCATTCCATCACCAAGTCT
  GGAAGAACCATCAGATGATGTGAAGAAAGGTGGAGGTTTACATAGCTCATCTCTTACTGTTGAGTGTTCC
  AAGACTTCAGAGAGTGAACCAAAGAATTTCACTGATGACCTTGGGCTCTCCATGACAGGGGATTCTTGCA
  AACTGATGCTTTCTACAAGTGAGTATAGTCAGTCCTCAAAGATGGAGAGCTTGGGTTCTCCCAGGACTGA
  GGAAGACAGAGAGAACACACAGATTGACGATACTGAACCTTTGTCTCCAGTTAGCAATTCTAAACTTCCT
  GCTGACAGTGAGAATGTCCTGGTGACTCCATCGCAGGACGACCAGGTAGAAATGAGTCAGAATGTAGATA
  AAGCAAAAGAGGATGAAACGGAGGACAGAGGTGACTGTAAAGGCAGAGAAGACGCGGTTGCTGAAGATGT
  TTGCATCGACCTCACTTGTGATTCTGGGAGTCAGGCAGTTCCTTCTCCAGCTACCCGTTCGGAGGCACTT
  TCCAGTGTCTTAGATCAGGAGGAAGCTATGGACACTAAAGAACACCATCCAGAGGAAGGGTTTTCGGGAT
  CTGAAGTAGAAGAAGTCCCTGAGACTCCCTGTGGAAGTCACAGAGAGGAGCCCAAAGAAGAACCGATGGA
  AAGTATCCCACTGCACCTTTCTCTGACTGAAACCCAGTCTGAGGCATTGTGTCTGCAGAAGGAAGCCCCC
  AAAGAGGAATGCCCAGAAGCTATGGAAGTTGAAACCAGTGTGATTAGTATTGACTCCCCCCAGAAACTGC
  AAGTACTTGACCAGGAATTAGAGCATAAGGACCCAGACACCTGGGAAGAAGCTACTTCTGAGGACTCCAG
  TGTTGTTATCGTTGATGTGAAGGAGCCCTCTCCTCGAGCTGATGTTTCCTGTGAACCTTTGGAGGAGGTG
  GAGAAATGCTCTGACTCCCAGTCCTGGGAGGGTGTGGCTCCAGAGGAAGAACCATGTGCTGAGAATAGAT
  TAGATACTCCAGAAGAAAAGCGTATAGAATGTGATGGAGATTCAAAAGCAGAGACCACAGAAAAGGACGC
  TGTAACAGAAGACTCTCCACAACCTCCTTTGCCTTCAGTGAGAGACGAACCTGTCAGACCCGATCAGGAG
  ACACAGCAGCCCCAAGTTCAAGAGAAAGAGAGCCCAGTGACTGTAGATGCAGAAGTGGCTGATGACAAGC
  AGCTGGGACCAGAGGGCGCATGCCAGCAGCTTGAGAAGGCCCCTGCCTGTGCCTCACAGAGTTTCTGTGA
  AAGTTCTAGTGAAACTCCATTTCATTTCACTTTGCCTAAAGAAGGTGATATTATCCCACCATTGACTGGC
  GCAACCCCACCTCTTATTGGGCACCTAAAATTGGAGCCCAAGAGACATAGTACTCCTATTGGGATTAGCA
  ACTATCCAGAAAGCACCATAGCAACCAGTGATGTCACATCTGAAAGCATGGTGGAGATCAATGATCCTCT
  ACTTGGGAATGAAAAAGGGGATTCTGAGTCTGCCCCAGAAATGGATGGAAAACTGTCTCTGAAAATGAAA
  CTGGTTAGTCCTGAGACAGAGGCCAGTGAAGAATCTTTGCAATTTAGCCTGGAAAAGCCTACAACTGCTG
  AGAGAAAAAATGGATCTACTGCCATTGCAGAGCCTGTTGCCAGTCTCCAGAAGCCGGTGCCTGTGTTTGG
  CTGCATCTATGAAGCCCAGCAAGAGAAGGAGGCTCAAAGTGAGGCTCCTCCCTCTGCACCCGACAGGGCG
  AATTTGCTCCATTTTCCAAGTGCTCAAGAAGAAGACAAAGAAAGACCAGATGTTACCCCAAAACTCAGGC
  AGAGTGAACAGCCTGTGAAGCCTGTTGGGCCTGTCATGGACGATGCTGCTCCTGAGGACTCAGCTTCTCC
  TGTTTCGCAGCAGAGGGCCTCACAGGAGCAGAGGGCCTCACAGGAGCCCTTCAGTCCTGCAGAGGACGTG
  ATGGAAACAGACCTGCTGGAAGGACTGGCTGCTAACCAGGACAGACCTAGTAAGATGTTGATGGACAGGC
  CCACCCAGAGTAACATCGGGATCCAGACCGTGGACCATTCCCTGTGCGCCCCAGAAACTGTCTCGGCAGC
  AACTCAGACTGTGAAGAGTGTGTGTGAGCAGGGGACCAGTACAGCGGAGCAGAACTCTGGGAAACAAGAT
  GCCACTGTGCAGACTGAGAGGGGGAGTGGAGAGAAACCTGCCAGTGCTCCCGTGGACGACACAGAGTCCC
  TCCACAGCCAGGGGGAAGAGGAATTTGAAATGCCCCAGCCTCCGCATGGCCATGTCTTGCATCGCCACAT
  GAGAACCATTCGAGAAGTCCGTACACTCGTCACCCGCGTCATCACAGATGTTTATTATGTGGATGGGACA
  GAAGTGGAAAGGAAAGTAACGGAGGAGACTGAAGAACCAATCGTAGAGTGTCAGGAATGTGAAACAGAAG
  TTTCCCCTTCACAGACTGGAGGTTCCTCTGGGGACCTGGGGGACATCAGCTCCTTCTCCTCCAAAGCTTC
  CAGCTCACACCACACATCCAGTGGAACAAGTCTCTCAGCCATACACAGCAGTGGCAGCTCAGGGCGAGGA
  GCTGGGCCACTCAAAGGAAAAGCCAGTGGGACAGAAGCTGCAGATTTTGCCTTACCCAGTTCCCGAGGAG
  GCCCAGGAAAACTGAGTCCTAGAAAAGGGATCAGTCAGACAGGGGCACCGGTGTGTGAGGAAGATGGTGA
  TGCAGGCCTTGGCATCAGACAGGGAGGGAAGGCTCCAGTTACACCTCGTGGGCGTGGGCGAAGGGGCCGC
  CCACCTTCTCGGACCACTGGAACCAGAGAAACAGTTGTCTCTGGTCCGTTGGGCGTAGAAGATATTTCAC
  CTAGCATGTCACCAGATGACAAGTCCTTCACCCGCATTATGCCTCGTGTGCCAGATTCTACCAAACGGAC
  GGATGCCAGTTCTAGTACTTTGCGGCGGAGTGATTCTCCAGAGATTCCTTTTCAGGCTGCTACTGGTTCC
  TCTGATGGCTTGGATTCCTCATCTTCAGGAAACAGCTTTGTGGGTCTCCGTGTTGTAGCTAAGTGGTCAT
  CCAATGGCTACTTTTACTCTGGGAAGATCACCCGAGATGTGGGGGCTGGGAAGTACAAGCTGCTCTTTGA
  TGATGGGTACGAATGTGACGTGCTGGGCAAAGACATTCTCCTGTGTGACCCCATCCCCCTGGACACTGAA
  GTGACAGCCCTCTCAGAAGATGAATATTTCAGTGCAGGAGTGGTGAAAGGACATAGGAAGGAGTCTGGGG
  AGCTGTATTACAGCATTGAAAAAGAAGGCCAAAGGAAGTGGTATAAACGAATGGCAGTCATTCTGTCCTT
  GGAGCAAGGAAACAGACTGAGAGAGCAATATGGGCTTGGCCCATATGAAGCTGTCACACCGCTCACGAAG
  GCAGCAGATATCAGCTTAGACAATTTGGTGGAAGGAAAGCGGAAACGGCGCAGTAACATCAGCTCCCCAG
  TCACCCCCACTGCCGCCAGTAGCAGCAGCACAACGCCCACACGTAAAGCCACGGAGAGTCCCCGTGCTTC
  CACGGGAGTCCCATCAGGCAAAAGGAAACTTCCGACTTCTGAAGAGGAACGCTCTCCTGCTAAGCGAGGT
  CGAAAGTCTGCCACCGTGAAACCTGGAACAGTGGGGGCAGCAGAGTTTGTGAGTCCCTGTGAGACTGGAG
  ACAACATAGGTGAGCCTTCTGTCCTAGAAGAGCCAAGAGGGCCTTTGCCCCTCAACAAGACCTTGTTTCT
  GGGCTATGCCTTTCTCCTCACCATGGCTACAACTAGTGACAAGCTGGCCAGTCGCTCTAAGCTGCTAGAT
  GGTCCTACAGGAAGCAGTGAAGAAGAGGAGGAATTTTTAGAAATTCCTCCTTTCAACAAGCAGTATACAG
  AATGCCAGCTTCGAGCAGGAGCTGGGTATATCCTTGAAGACTTCAATGAAGCCCAGTGTAACACAGCCTA
  CCAGTGTCTCCTAATTGCGGACCAGCACTGTCGAACCCGGAAGTACTTCCTGTGCCTTGCCAGTGGCATT
  CCTTGTGTGTCTCATGTCTGGGTCCATGACAGTTGCCATGCCAACCAACTTCAAAACTATCGTAATTATC
  TGCTGCCTGCTGGGTATAGCCTTGAAGAGCAACGAATTCTGGATTGGCAACCCCGTGAAAACCCTTTCCA
  GAATCTGAAGGTCCTCTTGGTGTCAGATCAACAACAGAACTTCTTGGAGCTCTGGTCTGAGATCCTTATG
  ACTGGAGGGGCAGCCTCTGTGAAGCAGCACCATTCAAGTGCCCACAACAAAGACATTGCTTTAGGGGTAT
  TTGATGTGGTGGTGACAGACCCCTCATGCCCAGCCTCGGTGCTCAAGTGTGCTGAAGCCTTGCAACTGCC
  TGTGGTATCACAAGAATGGGTGATCCAGTGCCTCATTGTTGGGGAGAGAATTGGATTCAAGCAGCATCCA
  AAATATAAACATGATTATGTTTCTCACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013735
• Insert Size: 5910 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013735.4, NP_038763.3
• RefSeq Size: 9652 bp
• RefSeq ORF: 5910 bp
• Locus ID: 27223
• UniProt ID: P70399
• Cytogenetics: 2 E5
• Gene Summary: Double-strand break (DSB) repair protein involved in response to DNA damage, telomere dynamics and class-switch recombination (CSR) during antibody genesis (PubMed:15159415, PubMed:15077110, PubMed:20453858, PubMed:23333305, PubMed:26308889, PubMed:20362325). Plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs and specifically counteracting the function of the homologous recombination (HR) repair protein BRCA1 (PubMed:23333305, PubMed:20362325). In response to DSBs, phosphorylation by ATM promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites. Recruited to DSBs sites by recognizing and binding histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites. Required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs (PubMed:15159415, PubMed:15077110). Participates to the repair and the orientation of the broken DNA ends during CSR (PubMed:26308889). In contrast, it is not required for classic NHEJ and V(D)J recombination (PubMed:15159415). Promotes NHEJ of dysfunctional telomeres (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). This variant is supported by the 5'-partial mRNA AJ414734.1, with inference of the first exon from other transcript alignments.