Hipk3 (NM_010434) Mouse Tagged ORF Clone

CAT#: MR225763

Hipk3 (Myc-DDK-tagged) - Mouse homeodomain interacting protein kinase 3 (Hipk3), transcript variant 1

Product Images

Circular map for MR225763

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hipk3
• Synonyms: DYRK6; FIST3; mir-1902; PKY
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225763 representing NM_010434
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCACAAGTCTTGGTCTACCCACCGTATGTTTATCAAACTCAGTCAAGTGCCTTTTGTAGTGTGA
  AGAAACTCAAAGTAGAGCCAAGCGGTTGTGTTTTCCAGGAAAGAACCTATCCTCAGATCCATGTGAATGG
  TAGAAACTTTGGCAATTCTCATCCTTCCACGAAAGGTAGTGCTTTCCAGACAAAGATACCATTTACTAAA
  CCTCGAGGACACAGCTTTTCATTGCAGGCAGGTGCCATTGTTGTCAAAGACACTGCCGGTGCTACAAAGG
  TCCTAGCAGCTCAGGCGCAGCAGGCTGGAGTGGAGGCACCGCGGGCCGTGGTGTGGAGAAACAGGTTACA
  TTTCCTAGAAGGGCCCCAGCGATGCGGGTTAAAGCGCAAGAGTGAGGAGTTGGAGAATCACAGCGGCGCG
  ATGCAGATTGTTGATGAACTGTCCATACTTCCTGCAATGTTGCAAACCAACATGGGAAACCCAGTGACAG
  TTGTGACAGCGACCACAGGATCGAAGCAGAACTGCACCAGCGGGGAAGGCGACTATCAGTTAGTGCAGCA
  TGAAGTCCTTTGCTCTATGAAAAACACGTATGAAGTCCTGGATTTTCTTGGTCGTGGCACTTTTGGCCAG
  GTTGTCAAATGCTGGAAGAGAGGGACAAATGAAATTGTAGCCATTAAAATTTTGAAGAATCACCCTTCGT
  ATGCACGTCAAGGGCAGATAGAAGTGAGCATCTTGGCAAGGCTGAGTACAGAGAACGCTGATGAGTATAA
  CTTTGTGCGAGCCTATGAGTGCTTCCAGCACCGTAACCACACCTGCCTAGTCTTTGAGATGCTGGAGCAG
  AACCTGTATGACTTCCTGAAACAGAATAAATTCAGTCCCCTGCCCCTGAAGGTGATCCGGCCTGTTCTTC
  AGCAAGTGGCCACTGCACTGAAGAAATTAAAAAGTCTTGGTTTAATTCATGCTGACCTCAAACCAGAGAA
  TATTATGTTGGTGGATCCTGTTCGCCAGCCGTACAGGGTTAAAGTGATAGACTTTGGATCGGCCAGTCAT
  GTATCAAAGACTGTTTGCTCAACGTATCTTCAATCTCGCTACTACAGAGCTCCAGAGATTATATTGGGGT
  TGCCGTTTTGTGAAGCCATAGACATGTGGTCACTGGGATGCGTGATCGCAGAGTTATTCCTTGGATGGCC
  ACTCTACCCAGGAGCCTTGGAGTATGATCAGATCCGATACATTTCTCAGACTCAAGGTTTACCAGGAGAG
  CAATTGTTGAATGTGGGTACAAAATCCACAAGATTTTTTTGCAGAGAAACAGATATGTCTCATTCTGGTT
  GGAGGTTAAAGACACTGGAAGAGCATGAGGCAGAGACAGGAATGAAGTCTAAAGAGGCCAGAAAGTACAT
  ATTCAACAGTCTGGACGATATAGTGCATGTGAACACAGTGATGGACTTGGAAGGAGGTGATCTCTTGGCT
  GAGAAAGCTGATAGAAGAGAATTTGTTAATCTGTTGAAGAAAATGTTGCTGATTGATGCAGATTTAAGAA
  TTACTCCAATTGAGACTCTGAACCACCCATTCGTTAATATGAAGCATCTACTAGATTTTCCTCACAGCAA
  CCATGTAAAGTCCTGTTTTCATATCATGGATATTTGTAAGTCTCCGAGTTCATGTGAAACAAATAACCAC
  AGTAAAATGTCACTCTTACGACCAGTCGCATCGAATGGCACTGCTGCTCTAGCAGCAAACTTTACTAAAG
  TCGGGACACTAAGAAGTCAGGCGTTAACCACATCTGCTCACTCTGTTGTGCACCATGGCATACCTCTGCA
  GGCGGGGACTGCACAGTTCGGTTGTGGAGATGCTTTTCATCAGACCCTGATTATCTGTCCCCCAGCTATC
  CAAGGTATTCCTGCAGCACATGGTAAACCCACCAGTTATTCCATAAGGGTAGACAATACAGTTCCGCTTG
  TAACTCAGGCCCCAGCTGTGCAGCCTCTGCAGATCCGACCTGGAGTCCTTTCACAGCAGACATGGTCTGG
  TCGAACACAGCAGATGCTAATACCTGCCTGGCAGCAGGTAACACCCATGGCTCCTGCTGCCGCAACACTA
  ACTTCTGAAGGCATGGCTGGTTCTCAGAGGCTTGGAGACTGGGGGAAAATGATTCCACACAGCAATCATT
  ACAACTCGGTGATGCCACCGCCTCTTCTAACCAACCAGATCACATTATCAGCCCCTCAGCCTATCAGCGT
  GGGCATTGCACATGTTGTCTGGCCTCAGCCTGCCACTACCAAGAAAAATAAGTTGTGCCAGAACAGGAGT
  AATTCATTGCAGAACACCAATATCCCACATTCAGCATTTATTTCTCCAAAGATAATCAGTGGGAAAGAGG
  TTGAGGAAGTAAGTTGTGTAGACACACAGGACAATCATACCTCAGAAGGAGAGGCCGGAACTTGCCGTGA
  AGCGTCTGTCAGACAGGATTCTTCAGTCTCAGACAAACAGCGGCAAACCATCATCATTGCCGACTCCCCG
  AGTCCTGCCGTGAGTGTGATCACCATTAGCAGTGACAGCGATGATGAAGAGACCTCACCCAGACCTTCAC
  TCCGAGAGTGTAAAGGTAGTCTAGATTGTGAAGCTTGCCAAAGCACTTTGAATATTGATCGGATGTGTTC
  ACTCAGCAGTCCTGATAGCACTCTGAGCACCAGCTCCTCAGGGCAGTCCAGCCCGTCCCCTTGCAAGAGA
  CCGAACAGCATGTCAGATGATGAACAAGAAAGCGGGTGTGAGACTGTGGATGGCTCTCCAACATCAGACT
  CCTCGGGGCACGACAGTCCATTTGCAGAGAACAGTTTTGTGGAGGATGCTCATCAGAACACAGAGCTGGG
  GACCTGTGCTGGCCCAGAAGCCAAGCCAGCTGTTGGCACTGCTGTGGAGCCACCGGTGGGGAGAGAAAGT
  GGACTGAGTGTTGACGAGCACATGGCAAACACGGATTCTACATGCCAGCCATTGAGAAAAGGCCAGCCTG
  CCCCCGGAAAGCTGCACCAGCCTCCTGCCCTGGGCGCTCGTCAGCAGAAGCCCGCAGCAGCGTTCCCTCA
  GCAGCATTTGAACCTGAGCCAGGTTCAGCATTTCGGAACTGGGCATCAAGAATGGAATGGAAACTTTGGG
  CATCGAAGACAGCAAGCATATATTCCTACTAGTGTTACCAGTAATCCATTCACGCTTTCTCATGGAAGTC
  CTAACCACACAGCAGTACATGCCCATCTGGCTGGAAGTACACACCTCGGAGGACAGCCTACTCTGCTTCC
  ATACCCATCATCAGCCTCCCTCAGTAGTGCTGCACCCGTGGCCCACCTCCTAGCCTCTCCGTGTACCTCA
  AGACCTATGTTACAGCATCCAACTTACAATATCTCCCATCCCAGTGGCATAGTTCACCAAGTCCCAGTGG
  GCATAAACCCCCGCCTGTTACCATCCCCAACCATTCATCAGACTCAGTACAAACCAATCTTCCCTCCACA
  TTCTTACATTGCAGCATCACCTGCGTATACTGGATTTCCATTGAGTCCAACAAAACTCAGCCAGTATCCA
  TATATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225763 representing NM_010434
  Red=Cloning site Green=Tags(s)
  
  MASQVLVYPPYVYQTQSSAFCSVKKLKVEPSGCVFQERTYPQIHVNGRNFGNSHPSTKGSAFQTKIPFTK
  PRGHSFSLQAGAIVVKDTAGATKVLAAQAQQAGVEAPRAVVWRNRLHFLEGPQRCGLKRKSEELENHSGA
  MQIVDELSILPAMLQTNMGNPVTVVTATTGSKQNCTSGEGDYQLVQHEVLCSMKNTYEVLDFLGRGTFGQ
  VVKCWKRGTNEIVAIKILKNHPSYARQGQIEVSILARLSTENADEYNFVRAYECFQHRNHTCLVFEMLEQ
  NLYDFLKQNKFSPLPLKVIRPVLQQVATALKKLKSLGLIHADLKPENIMLVDPVRQPYRVKVIDFGSASH
  VSKTVCSTYLQSRYYRAPEIILGLPFCEAIDMWSLGCVIAELFLGWPLYPGALEYDQIRYISQTQGLPGE
  QLLNVGTKSTRFFCRETDMSHSGWRLKTLEEHEAETGMKSKEARKYIFNSLDDIVHVNTVMDLEGGDLLA
  EKADRREFVNLLKKMLLIDADLRITPIETLNHPFVNMKHLLDFPHSNHVKSCFHIMDICKSPSSCETNNH
  SKMSLLRPVASNGTAALAANFTKVGTLRSQALTTSAHSVVHHGIPLQAGTAQFGCGDAFHQTLIICPPAI
  QGIPAAHGKPTSYSIRVDNTVPLVTQAPAVQPLQIRPGVLSQQTWSGRTQQMLIPAWQQVTPMAPAAATL
  TSEGMAGSQRLGDWGKMIPHSNHYNSVMPPPLLTNQITLSAPQPISVGIAHVVWPQPATTKKNKLCQNRS
  NSLQNTNIPHSAFISPKIISGKEVEEVSCVDTQDNHTSEGEAGTCREASVRQDSSVSDKQRQTIIIADSP
  SPAVSVITISSDSDDEETSPRPSLRECKGSLDCEACQSTLNIDRMCSLSSPDSTLSTSSSGQSSPSPCKR
  PNSMSDDEQESGCETVDGSPTSDSSGHDSPFAENSFVEDAHQNTELGTCAGPEAKPAVGTAVEPPVGRES
  GLSVDEHMANTDSTCQPLRKGQPAPGKLHQPPALGARQQKPAAAFPQQHLNLSQVQHFGTGHQEWNGNFG
  HRRQQAYIPTSVTSNPFTLSHGSPNHTAVHAHLAGSTHLGGQPTLLPYPSSASLSSAAPVAHLLASPCTS
  RPMLQHPTYNISHPSGIVHQVPVGINPRLLPSPTIHQTQYKPIFPPHSYIAASPAYTGFPLSPTKLSQYP
  YM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010434
• ORF Size: 3576 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010434.2, NP_034564.2
• RefSeq Size: 7497 bp
• RefSeq ORF: 3579 bp
• Locus ID: 15259
• UniProt ID: Q9ERH7
• Cytogenetics: 2 E2
• MW: 130.5 kDa
• Gene Summary: Serine/threonine-protein kinase involved in transcription regulation, apoptosis and steroidogenic gene expression. Phosphorylates JUN and RUNX2. Seems to negatively regulate apoptosis by promoting FADD phosphorylation. Enhances androgen receptor-mediated transcription. May act as a transcriptional corepressor for NK homeodomain transcription factors. The phosphorylation of NR5A1 activates SF1 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. In osteoblasts, supports transcription activation: phosphorylates RUNX2 that synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE).[UniProtKB/Swiss-Prot Function]