Hipk3 (NM_010434) Mouse Tagged ORF Clone

CAT#: MG225763

Hipk3 (tGFP-tagged) - Mouse homeodomain interacting protein kinase 3 (Hipk3) transcript variant 1, (10ug)

Product Images

Circular map for MG225763

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Hipk3
• Synonyms: DYRK6; FIST3; mir-1902; PKY
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225763 representing NM_010434
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCACAAGTCTTGGTCTACCCACCGTATGTTTATCAAACTCAGTCAAGTGCCTTTTGTAGTGTGA
  AGAAACTCAAAGTAGAGCCAAGCGGTTGTGTTTTCCAGGAAAGAACCTATCCTCAGATCCATGTGAATGG
  TAGAAACTTTGGCAATTCTCATCCTTCCACGAAAGGTAGTGCTTTCCAGACAAAGATACCATTTACTAAA
  CCTCGAGGACACAGCTTTTCATTGCAGGCAGGTGCCATTGTTGTCAAAGACACTGCCGGTGCTACAAAGG
  TCCTAGCAGCTCAGGCGCAGCAGGCTGGAGTGGAGGCACCGCGGGCCGTGGTGTGGAGAAACAGGTTACA
  TTTCCTAGAAGGGCCCCAGCGATGCGGGTTAAAGCGCAAGAGTGAGGAGTTGGAGAATCACAGCGGCGCG
  ATGCAGATTGTTGATGAACTGTCCATACTTCCTGCAATGTTGCAAACCAACATGGGAAACCCAGTGACAG
  TTGTGACAGCGACCACAGGATCGAAGCAGAACTGCACCAGCGGGGAAGGCGACTATCAGTTAGTGCAGCA
  TGAAGTCCTATGCTCTATGAAAAACACGTATGAAGTCCTGGATTTTCTTGGTCGTGGCACTTTTGGCCAG
  GTTGTCAAATGCTGGAAGAGAGGGACAAATGAAATTGTAGCCATTAAAATTTTGAAGAATCACCCTTCGT
  ATGCACGTCAAGGGCAGATAGAAGTGAGCATCTTGGCAAGGCTGAGTACAGAGAACGCTGATGAGTATAA
  CTTTGTGCGAGCCTATGAGTGCTTCCAGCACCGTAACCACACCTGCCTAGTCTTTGAGATGCTGGAGCAG
  AACCTGTATGACTTCCTGAAACAGAATAAATTCAGTCCCCTGCCCCTGAAGGTGATCCGGCCTGTTCTTC
  AGCAAGTGGCCACTGCACTGAAGAAATTAAAAAGTCTTGGTTTAATTCATGCTGACCTCAAACCAGAGAA
  TATTATGTTGGTGGATCCTGTTCGCCAGCCGTACAGGGTTAAAGTGATAGACTTTGGATCGGCCAGTCAT
  GTATCAAAGACTGTTTGCTCAACGTATCTTCAATCTCGCTACTACAGAGCTCCAGAGATTATATTGGGGT
  TGCCGTTTTGTGAAGCCATAGACATGTGGTCACTGGGATGCGTGATCGCAGAGTTATTCCTTGGATGGCC
  ACTCTACCCAGGAGCCTTGGAGTATGATCAGATCCGATACATTTCTCAGACTCAAGGTTTACCAGGAGAG
  CAATTGTTGAATGTGGGTACAAAATCCACAAGATTTTTTTGCAGAGAAACAGATATGTCTCATTCTGGTT
  GGAGGTTAAAGACACTGGAAGAGCATGAGGCAGAGACAGGAATGAAGTCTAAAGAGGCCAGAAAGTACAT
  ATTCAACAGTCTGGACGATATAGTGCATGTGAACACAGTGATGGACTTGGAAGGAGGTGATCTCTTGGCT
  GAGAAAGCTGATAGAAGAGAATTTGTTAATCTGTTGAAGAAAATGTTGCTGATTGATGCAGATTTAAGAA
  TTACTCCAATTGAGACTCTGAACCACCCATTCGTTAATATGAAGCATCTACTAGATTTTCCTCACAGCAA
  CCATGTAAAGTCCTGTTTTCATATCATGGATATTTGTAAGTCTCCGAGTTCATGTGAAACAAATAACCAC
  AGTAAAATGTCACTCTTACGACCAGTCGCATCGAATGGCACTGCTGCTCTAGCAGCAAACTTTACTAAAG
  TCGGGACACTAAGAAGTCAGGCGTTAACCACATCTGCTCACTCTGTTGTGCACCATGGCATACCTCTGCA
  GGCGGGGACTGCACAGTTCGGTTGTGGAGATGCTTTTCATCAGACCCTGATTATCTGTCCCCCAGCTATC
  CAAGGTATTCCTGCAGCACATGGTAAACCCACCAGTTATTCCATAAGGGTAGACAATACAGTTCCGCTTG
  TAACTCAGGCCCCAGCTGTGCAGCCTCTGCAGATCCGACCTGGAGTCCTTTCACAGACATGGTCTGGTCG
  AACACAGCAGATGCTAATTCCTGCCTGGCAGCAGGTAACACCCATGGCTCCTGCTGCCGCAACACTAACT
  TCTGAAGGCATGGCTGGTTCTCAGAGGCTTGGAGACTGGGGGAAAATGATTCCACACAGCAATCATTACA
  ACTCGGTGATGCCACCGCCTCTTCTAACCAACCAGATCACATTATCAGCCCCTCAGCCTATCAGCGTGGG
  CATTGCACATGTTGTCTGGCCTCAGCCTGCCACTACCAAGAAAAATAAGCTGTGCCAGAACAGGAGTAAT
  TCATTGCAGAACACCAATATCCCACATTCAGCATTTATTTCTCCAAAGATAATCAGTGGGAAAGAGGTTG
  AGGAAGTAAGTTGTGTAGACACACAGGACAATCATACCTCAGAAGGAGAGGCCAGAACTTGTCGTGAAGC
  GTCTGTCAGACAGGATTCTTCAGTCTCAGACAAACAGCGGCAAACCATCATCATTGCCGACTCCCCGAGT
  CCTGCCGTGAGTGTGATCACCATTAGCAGTGACAGCGATGATGAAGAGACCTCACCCAGACCTTCACTCC
  GAGAGTGTAAAGGTAGTCTAGATTGTGAAGCTTGCCAAAGCACTTTGAATATTGATCGGATGTGTTCACT
  CAGCAGTCCTGATAGCACTCTGAGCACCAGCTCCTCAGGGCAGTCCAGCCCGTCCCCTTGCAAGAGACCG
  AACAGTATGTCAGATGATGAACAAGAAAGCGGGTGTGAGACTGTGGATGGCTCTCCAACATCAGACTCCT
  CGGGGCACGACAGTCCATTTGCAGAGAACAGTTTTGTGGAGGATGCTCATCAGAACACAGAGCTGGGGAC
  CTGTGCTGGCCCAGAAGCCAAGCCAGCTGTTGGCACTGCTGTGGAGCCACCGGTGGGGAGAGAAAGTGGA
  CTGAGTGTTGACGAGCACATGGCAAACACGGATTCTACATGCCAGCCATTGAGAAAAGGCCAGCCTGCCC
  CCGGAAAGCTGCACCAGCCTCCTGCCCTGGGCGCTCGTCAGCAGAAGCCCGCAGCAGCGTTCCCTCAGCA
  GCATTTGAACCTGAGCCAGGTTCAGCATTTCGGAACTGGGCATCAAGAATGGAATGGAAACTTTGGGCAT
  CGAAGACAGCAAGCATATATTCCTACTAGTGTTACCAGTAATCCATTCACGCTTTCTCATGGAAGTCCTA
  ACCACACAGCAGTACATGCCCATCTGGCTGGAAGTACACACCTCGGAGGACAGCCTACTCTGCTTCCATA
  CCCATCATCAGCCTCCCTCAGTAGTGCTGCACCCGTGGCCCACCTCCTAGCCTCTCCGTGTACCTCAAGA
  CCTATGTTACAGCATCCAACTTACAATATCTCCCATCCCAGTGGCATAGTTCACCAAGTCCCAGTGGGCA
  TAAACCCCCGCCTGTTACCATCCCCAACCATTCATCAGACTCAGTACAAACCAATCTTCCCTCCACATTC
  TTACATTGCAGCATCACCTGCGTATACTGGATTTCCATTGAGTCCAACAAAACTCAGCCAGTATCCATAT
  ATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225763 representing NM_010434
  Red=Cloning site Green=Tags(s)
  
  MASQVLVYPPYVYQTQSSAFCSVKKLKVEPSGCVFQERTYPQIHVNGRNFGNSHPSTKGSAFQTKIPFTK
  PRGHSFSLQAGAIVVKDTAGATKVLAAQAQQAGVEAPRAVVWRNRLHFLEGPQRCGLKRKSEELENHSGA
  MQIVDELSILPAMLQTNMGNPVTVVTATTGSKQNCTSGEGDYQLVQHEVLCSMKNTYEVLDFLGRGTFGQ
  VVKCWKRGTNEIVAIKILKNHPSYARQGQIEVSILARLSTENADEYNFVRAYECFQHRNHTCLVFEMLEQ
  NLYDFLKQNKFSPLPLKVIRPVLQQVATALKKLKSLGLIHADLKPENIMLVDPVRQPYRVKVIDFGSASH
  VSKTVCSTYLQSRYYRAPEIILGLPFCEAIDMWSLGCVIAELFLGWPLYPGALEYDQIRYISQTQGLPGE
  QLLNVGTKSTRFFCRETDMSHSGWRLKTLEEHEAETGMKSKEARKYIFNSLDDIVHVNTVMDLEGGDLLA
  EKADRREFVNLLKKMLLIDADLRITPIETLNHPFVNMKHLLDFPHSNHVKSCFHIMDICKSPSSCETNNH
  SKMSLLRPVASNGTAALAANFTKVGTLRSQALTTSAHSVVHHGIPLQAGTAQFGCGDAFHQTLIICPPAI
  QGIPAAHGKPTSYSIRVDNTVPLVTQAPAVQPLQIRPGVLSQTWSGRTQQMLIPAWQQVTPMAPAAATLT
  SEGMAGSQRLGDWGKMIPHSNHYNSVMPPPLLTNQITLSAPQPISVGIAHVVWPQPATTKKNKLCQNRSN
  SLQNTNIPHSAFISPKIISGKEVEEVSCVDTQDNHTSEGEARTCREASVRQDSSVSDKQRQTIIIADSPS
  PAVSVITISSDSDDEETSPRPSLRECKGSLDCEACQSTLNIDRMCSLSSPDSTLSTSSSGQSSPSPCKRP
  NSMSDDEQESGCETVDGSPTSDSSGHDSPFAENSFVEDAHQNTELGTCAGPEAKPAVGTAVEPPVGRESG
  LSVDEHMANTDSTCQPLRKGQPAPGKLHQPPALGARQQKPAAAFPQQHLNLSQVQHFGTGHQEWNGNFGH
  RRQQAYIPTSVTSNPFTLSHGSPNHTAVHAHLAGSTHLGGQPTLLPYPSSASLSSAAPVAHLLASPCTSR
  PMLQHPTYNISHPSGIVHQVPVGINPRLLPSPTIHQTQYKPIFPPHSYIAASPAYTGFPLSPTKLSQYPY
  M
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010434
• ORF Size: 3576 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010434.2, NP_034564.2
• RefSeq Size: 7497 bp
• RefSeq ORF: 3579 bp
• Locus ID: 15259
• UniProt ID: Q9ERH7
• Cytogenetics: 2 E2
• Gene Summary: Serine/threonine-protein kinase involved in transcription regulation, apoptosis and steroidogenic gene expression. Phosphorylates JUN and RUNX2. Seems to negatively regulate apoptosis by promoting FADD phosphorylation. Enhances androgen receptor-mediated transcription. May act as a transcriptional corepressor for NK homeodomain transcription factors. The phosphorylation of NR5A1 activates SF1 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. In osteoblasts, supports transcription activation: phosphorylates RUNX2 that synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE).[UniProtKB/Swiss-Prot Function]