Csf2rb2 (NM_007781) Mouse Tagged ORF Clone

CAT#: MR225402

Csf2rb2 (Myc-DDK-tagged) - Mouse colony stimulating factor 2 receptor, beta 2, low-affinity (granulocyte-macrophage) (Csf2rb2)

Product Images

Circular map for MR225402

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Csf2rb2
• Synonyms: AIC2A; BetaIl3; Bil3; Csfgmrb; Il3r; Il3rb; Il3rb2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225402 representing NM_007781
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCAGCAAATGGCACTCACATGGGGGCTGTGCTACATGGCACTGGTGGCTCTCTGCTGGGGACACG
  AGGTGACAGAGGAAGAAGAAACGGTCCCTCTGAAGACTCTGGAGTGCTACAATGACTACACCAACCGTAT
  CATCTGCAGCTGGGCAGACACAGAGGATGCCCAGGGGCTAATCAACATGACCCTCCTCTATCACCAGCTA
  GACAAGATTCAATCAGTGTCCTGTGAGCTCAGTGAGAAACTCATGTGGTCAGAGTGCCCGTCATCCCACC
  GCTGTGTGCCCAGAAGATGTGTCATCCCCTACACACGATTTTCTAATGGAGATAACGACTACTACTCCTT
  CCAGCCAGATCGTGACCTGGGCATCCAGCTCATGGTCCCACTGGCCCAGCATGTGCAGCCACCCCCTCCC
  AAGGACATCCACATCAGCCCCTCTGGGGATCATTTCCTGCTGGAATGGAGTGTATCTCTTGGGGATTCCC
  AGGTCTCCTGGCTTTCATCAAAGGACATAGAGTTTGAGGTGGCTTATAAGCGGCTTCAGGACTCCTGGGA
  GGATGCCTCCAGTCTCCACACTAGCAACTTTCAGGTGAATTTAGAGCCAAAGCTATTCCTACCCAACAGC
  ATCTATGCTGCCCGTGTGCGCACTCGGCTGTCCGCGGGTTCAAGCTTGTCTGGGAGACCCAGCAGATGGA
  GCCCAGAGGTTCACTGGGACTCCCAGCCAGGGGACAAGGCCCAGCCACAGAACCTTCAATGCTTCTTTGA
  TGGGATCCAGTCTCTCCACTGCTCCTGGGAGGTGTGGACCCAGACGACTGGCTCTGTTTCCTTTGGGCTC
  TTCTATCGCCCCAGCCCTGCAGCTCCGGAGGAGAAATGCTCTCCGGTGGTGAAGGAGCCGCAGGCCAGCG
  TCTACACCCGCTACCGCTGCAGTCTACCTGTGCCTGAGCCCAGTGCACACAGCCAGTACACAGTCTCTGT
  TAAGCACCTGGAACAAGGGAAGTTCATCATGAGCTATTACCACATCCAGATGGAACCTCCAATCCTCAAC
  CAGACCAAGAACAGAGACAGCTACAGCCTGCATTGGGAAACTCAGAAAATACCCAAATACATTGATCACA
  CTTTCCAGGTCCAGTACAAGAAGAAGTCAGAGAGCTGGAAGGACAGCAAGACAGAAAACCTAGGTCGAGT
  CAATAGCATGGACCTGCCCCAGCTGGAGCCGGACACCTCATACTGCGCCAGGGTGAGGGTCAAGCCCATC
  TCTGACTACGACGGGATCTGGAGCGAGTGGAGCAATGAGTACACTTGGACGACTGACTGGGTGATGCCCA
  CGCTGTGGATAGTCCTCATCCTGGTCTTTCTCATCTTCACCTTGCTCCTGGCTCTCCACTTTGGCCGTGT
  TTATGGGTACAGGACATACAGGAAGTGGAAGGAAAAGATCCCCAACCCCAGCAAGAGCCTCCTGTTCCAG
  GATGGAGGTAAAGGTCTCTGGCCTCCTGGCAGCATGGCAGCCTTCGCGACTAAGAACCCCGCTCTCCAGG
  GGCCACAGAGCAGGCTTCTTGCTGAGCAACAGGGGGTGTCATATGAACATTTGGAAGACAACAACGTGTC
  ACCTCTCACTATAGAGGACCCTAATATAATTCGAGATCCACCATCCAGGCCTGATACAACCCCAGCTGCC
  TCATCTGAATCCACAGAGCAACTTCCCAATGTTCAAGTAGAGGGACCAATTCCTTCTAGCCGACCCAGGA
  AGCAATTACCCAGCTTTGACTTCAATGGGCCCTACCTGGGGCCTCCCCAATCCCACTCCCTGCCTGATCT
  CCCAGGCCAGCTGGGTTCTCCCCAGGTGGGTGGGAGCCTGAAGCCAGCACTGCCAGGCTCCTTGGAGTAC
  ATGTGTCTGCCCCCTGGAGGTCAAGTGCAACTGGTCCCATTGTCCCAGGTGATGGGACAGGGCCAGGCTA
  TGGATGTGCAGTGTGGGTCCAGCCTGGAGACCACAGGGAGCCCTTCCGTGGAGCCAAAGGAGAACCCTCC
  AGTTGAGCTGAGCGTGGAGAAACAGGAGGCAAGGGACAACCCAATGACTCTTCCCATAAGCTCTGGGGGC
  CCTGAGGGCAGTATGATGGCCTCTGATTATGTCACTCCTGGAGATCCGGTGCTCACTCTGCCCACAGGGC
  CCCTGTCTACCTCTCTGGGCCCCTCTCTAGGGTTGCCCTCAGCCCAAAGCCCCAGTCTCTGTCTTAAGCT
  GCCCAGGGTCCCCTCTGGAAGCCCAGCTCTAGGGCCACCAGGGTTTGAGGACTATGTGGAGCTGCCTCCA
  AGTGTGAGCCAGGCAGCCACGTCCCCTCCAGGCCATCCTGCTCCTCCTGTGGCAAGCAGCCCCACAGTGA
  TCCCAGGAGAGCCCAGGGAGGAAGTGGGCCCAGCATCCCCACATCCCGAAGGCCTCCTTGTTCTTCAGCA
  GGTTGGGGACTACTGCTTCCTCCCTGGCCTGGGACCTGGCTCCCTCTCACCACACAGTAAGCCACCCTCT
  CCAAGTCTGTGTTCTGAGACTGAGGACCTAGACCAGGACTTGTCTGTGAAAAAGTTTCCCTATCAACCCT
  TGCCCCAGGCCCCAGCCATTCAGTTTTTCAAGTCCCTAAAGTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225402 representing NM_007781
  Red=Cloning site Green=Tags(s)
  
  MDQQMALTWGLCYMALVALCWGHEVTEEEETVPLKTLECYNDYTNRIICSWADTEDAQGLINMTLLYHQL
  DKIQSVSCELSEKLMWSECPSSHRCVPRRCVIPYTRFSNGDNDYYSFQPDRDLGIQLMVPLAQHVQPPPP
  KDIHISPSGDHFLLEWSVSLGDSQVSWLSSKDIEFEVAYKRLQDSWEDASSLHTSNFQVNLEPKLFLPNS
  IYAARVRTRLSAGSSLSGRPSRWSPEVHWDSQPGDKAQPQNLQCFFDGIQSLHCSWEVWTQTTGSVSFGL
  FYRPSPAAPEEKCSPVVKEPQASVYTRYRCSLPVPEPSAHSQYTVSVKHLEQGKFIMSYYHIQMEPPILN
  QTKNRDSYSLHWETQKIPKYIDHTFQVQYKKKSESWKDSKTENLGRVNSMDLPQLEPDTSYCARVRVKPI
  SDYDGIWSEWSNEYTWTTDWVMPTLWIVLILVFLIFTLLLALHFGRVYGYRTYRKWKEKIPNPSKSLLFQ
  DGGKGLWPPGSMAAFATKNPALQGPQSRLLAEQQGVSYEHLEDNNVSPLTIEDPNIIRDPPSRPDTTPAA
  SSESTEQLPNVQVEGPIPSSRPRKQLPSFDFNGPYLGPPQSHSLPDLPGQLGSPQVGGSLKPALPGSLEY
  MCLPPGGQVQLVPLSQVMGQGQAMDVQCGSSLETTGSPSVEPKENPPVELSVEKQEARDNPMTLPISSGG
  PEGSMMASDYVTPGDPVLTLPTGPLSTSLGPSLGLPSAQSPSLCLKLPRVPSGSPALGPPGFEDYVELPP
  SVSQAATSPPGHPAPPVASSPTVIPGEPREEVGPASPHPEGLLVLQQVGDYCFLPGLGPGSLSPHSKPPS
  PSLCSETEDLDQDLSVKKFPYQPLPQAPAIQFFKSLKY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007781
• ORF Size: 2634 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007781.3, NP_031807.1
• RefSeq Size: 4740 bp
• RefSeq ORF: 2637 bp
• Locus ID: 12984
• UniProt ID: P26954
• Cytogenetics: 15 37.22 cM
• MW: 97.2 kDa
• Gene Summary: In mouse, there are two classes of high-affinity IL3 receptors. One contains this IL3-specific beta subunit and the other contains the beta subunit also shared by high-affinity IL5 and GM-CSF receptors.[UniProtKB/Swiss-Prot Function]