Nr5a2 (NM_001159769) Mouse Tagged ORF Clone

CAT#: MR225372

Nr5a2 (Myc-DDK-tagged) - Mouse nuclear receptor subfamily 5, group A, member 2 (Nr5a2), transcript variant 2

Product Images

Circular map for MR225372

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nr5a2
• Synonyms: AU020803; D1Ertd308e; Ftf; LRH-1; UF2-H3B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225372 representing NM_001159769
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGCTAGTTTGGATACTGGAGATTTTCAAGAATTTCTTAAGCATGGACTTACAGCTATTGCGTCTG
  CACCAGGGTCAGAGACTCGCCACTCCCCCAAACGTGAGGAACAACTCCGGGAAAAACGTGCTGGGCTTCC
  GGACCGACACCGACGCCCCATTCCCGCCCGCAGCCGCCTTGTCATGCTGCCCAAAGTGGAGACGGAAGCC
  CCAGGACTGGTCCGATCGCATGGGGAACAGGGGCAGATGCCAGAAAACATGCAAGTGTCTCAATTTAAAA
  TGGTGAATTACTCCTATGATGAAGATCTGGAAGAGCTATGTCCTGTGTGTGGCGATAAAGTGTCTGGGTA
  CCATTACGGTCTCCTCACGTGCGAAAGCTGCAAGGGTTTTTTTAAGCGAACTGTCCAAAACCAAAAAAGG
  TACACGTGCATAGAGAACCAGAATTGCCAAATTGACAAAACGCAGAGAAAACGATGTCCCTACTGTCGAT
  TCAAAAAATGTATCGATGTTGGGATGAAGCTGGAAGCCGTAAGAGCCGACCGCATGCGAGGGGGCAGAAA
  TAAGTTTGGGCCAATGTACAAGAGAGACAGGGCTTTGAAGCAGCAGAAGAAAGCCCTCATTCGAGCCAAT
  GGACTTAAGCTGGAAGCCATGTCTCAGGTGATCCAAGCAATGCCCTCAGACCTGACCTCTGCAATTCAGA
  ACATTCATTCCGCCTCCAAAGGCCTACCTCTGAGCCATGTAGCCTTGCCTCCGACAGACTATGACAGAAG
  TCCCTTTGTCACATCTCCCATTAGCATGACAATGCCACCTCACAGCAGCCTGCATGGTTACCAACCCTAT
  GGTCACTTTCCTAGTCGGGCCATCAAGTCTGAGTACCCAGACCCCTACTCCAGCTCACCTGAGTCAATGA
  TGGGTTACTCCTACATGGATGGTTACCAGACAAACTCCCCGGCCAGCATCCCACACCTGATACTGGAACT
  TTTGAAGTGTGAACCAGATGAGCCTCAAGTTCAAGCGAAGATCATGGCTTACCTCCAGCAAGAGCAGAGT
  AACCGAAACAGGCAAGAAAAGCTGAGCGCATTTGGGCTTTTATGCAAAATGGCGGACCAGACCCTGTTCT
  CCATTGTTGAGTGGGCCAGGAGTAGTATCTTCTTCAGGGAACTGAAGGTTGATGACCAAATGAAGCTGCT
  TCAAAACTGCTGGAGTGAGCTCTTGATTCTCGATCACATTTACCGACAAGTGGCGCATGGGAAGGAAGGG
  ACAATCTTCCTGGTTACTGGAGAACACGTGGACTACTCCACCATCATCTCACACACAGAAGTCGCGTTCA
  ACAACCTCCTGAGTCTCGCACAGGAGCTGGTGGTGAGGCTCCGTTCCCTTCAGTTCGATCAGCGGGAGTT
  TGTATGTCTCAAGTTCCTGGTGCTGTTCAGCTCAGATGTGAAGAACCTGGAGAACCTGCAGCTGGTGGAA
  GGTGTCCAAGAGCAGGTGAATGCCGCCCTGCTGGACTACACGGTTTGCAACTACCCACAACAGACTGAGA
  AATTCGGACAGCTACTTCTTCGGCTACCCGAGATCCGGGCAATCAGCAAGCAGGCAGAAGACTACCTGTA
  CTATAAGCACGTGAACGGGGATGTGCCCTATAATAACCTCCTCATTGAGATGCTGCATGCCAAAAGAGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225372 representing NM_001159769
  Red=Cloning site Green=Tags(s)
  
  MSASLDTGDFQEFLKHGLTAIASAPGSETRHSPKREEQLREKRAGLPDRHRRPIPARSRLVMLPKVETEA
  PGLVRSHGEQGQMPENMQVSQFKMVNYSYDEDLEELCPVCGDKVSGYHYGLLTCESCKGFFKRTVQNQKR
  YTCIENQNCQIDKTQRKRCPYCRFKKCIDVGMKLEAVRADRMRGGRNKFGPMYKRDRALKQQKKALIRAN
  GLKLEAMSQVIQAMPSDLTSAIQNIHSASKGLPLSHVALPPTDYDRSPFVTSPISMTMPPHSSLHGYQPY
  GHFPSRAIKSEYPDPYSSSPESMMGYSYMDGYQTNSPASIPHLILELLKCEPDEPQVQAKIMAYLQQEQS
  NRNRQEKLSAFGLLCKMADQTLFSIVEWARSSIFFRELKVDDQMKLLQNCWSELLILDHIYRQVAHGKEG
  TIFLVTGEHVDYSTIISHTEVAFNNLLSLAQELVVRLRSLQFDQREFVCLKFLVLFSSDVKNLENLQLVE
  GVQEQVNAALLDYTVCNYPQQTEKFGQLLLRLPEIRAISKQAEDYLYYKHVNGDVPYNNLLIEMLHAKRA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001159769
• ORF Size: 1680 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 3635 bp
• RefSeq ORF: 1500 bp
• Locus ID: 26424
• Cytogenetics: 1 E4
• MW: 57.6 kDa
• Gene Summary: Nuclear receptor that acts as a key metabolic sensor by regulating the expression of genes involved in bile acid synthesis, cholesterol homeostasis and triglyceride synthesis. Together with the oxysterol receptors NR1H3/LXR-alpha and NR1H2/LXR-beta, acts as an essential transcriptional regulator of lipid metabolism. Plays an anti-inflammatory role during the hepatic acute phase response by acting as a corepressor: inhibits the hepatic acute phase response by preventing dissociation of the N-Cor corepressor complex. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development (By similarity). Activates the transcription of CYP2C38 (PubMed:30555544).[UniProtKB/Swiss-Prot Function]