Nr5a2 (NM_001159769) Mouse Untagged Clone

CAT#: MC216985

Nr5a2 (untagged) - Mouse nuclear receptor subfamily 5, group A, member 2 (Nr5a2), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nr5a2
• Synonyms: AU020803; D1Ertd308e; Ftf; LRH-1; UF2-H3B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216985 representing NM_001159769
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCCCAAAGTGGAGACGGAAGCCCCAGGACTGGTCCGATCGCATGGGGAACAGGGGCAGATGCCAG
  AAAACATGCAAGTGTCTCAATTTAAAATGGTGAATTACTCCTATGATGAAGATCTGGAAGAGCTATGTCC
  TGTGTGTGGCGATAAAGTGTCTGGGTACCATTACGGTCTCCTCACGTGCGAAAGCTGCAAGGGTTTTTTT
  AAGCGAACTGTCCAAAACCAAAAAAGGTACACGTGCATAGAGAACCAGAATTGCCAAATTGACAAAACGC
  AGAGAAAACGATGTCCCTACTGTCGATTCAAAAAATGTATCGATGTTGGGATGAAGCTGGAAGCCGTAAG
  AGCCGACCGCATGCGAGGGGGCAGAAATAAGTTTGGGCCAATGTACAAGAGAGACAGGGCTTTGAAGCAG
  CAGAAGAAAGCCCTCATTCGAGCCAATGGACTTAAGCTGGAAGCCATGTCTCAGGTGATCCAAGCAATGC
  CCTCAGACCTGACCTCTGCAATTCAGAACATTCATTCCGCCTCCAAAGGCCTACCTCTGAGCCATGTAGC
  CTTGCCTCCGACAGACTATGACAGAAGTCCCTTTGTCACATCTCCCATTAGCATGACAATGCCACCTCAC
  AGCAGCCTGCATGGTTACCAACCCTATGGTCACTTTCCTAGTCGGGCCATCAAGTCTGAGTACCCAGACC
  CCTACTCCAGCTCACCTGAGTCAATGATGGGTTACTCCTACATGGATGGTTACCAGACAAACTCCCCGGC
  CAGCATCCCACACCTGATACTGGAACTTTTGAAGTGTGAACCAGATGAGCCTCAAGTTCAAGCGAAGATC
  ATGGCTTACCTCCAGCAAGAGCAGAGTAACCGAAACAGGCAAGAAAAGCTGAGCGCATTTGGGCTTTTAT
  GCAAAATGGCGGACCAGACCCTGTTCTCCATTGTTGAGTGGGCCAGGAGTAGTATCTTCTTCAGGGAACT
  GAAGGTTGATGACCAAATGAAGCTGCTTCAAAACTGCTGGAGTGAGCTCTTGATTCTCGATCACATTTAC
  CGACAAGTGGCGCATGGGAAGGAAGGGACAATCTTCCTGGTTACTGGAGAACACGTGGACTACTCCACCA
  TCATCTCACACACAGAAGTCGCGTTCAACAACCTCCTGAGTCTCGCACAGGAGCTGGTGGTGAGGCTCCG
  TTCCCTTCAGTTCGATCAGCGGGAGTTTGTATGTCTCAAGTTCCTGGTGCTGTTCAGCTCAGATGTGAAG
  AACCTGGAGAACCTGCAGCTGGTGGAAGGTGTCCAAGAGCAGGTGAATGCCGCCCTGCTGGACTACACGG
  TTTGCAACTACCCACAACAGACTGAGAAATTCGGACAGCTACTTCTTCGGCTACCCGAGATCCGGGCAAT
  CAGCAAGCAGGCAGAAGACTACCTGTACTATAAGCACGTGAACGGGGATGTGCCCTATAATAACCTCCTC
  ATTGAGATGCTGCATGCCAAAAGAGCCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001159769
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001159769.2, NP_001153241.1
• RefSeq Size: 3635 bp
• RefSeq ORF: 1500 bp
• Locus ID: 26424
• Cytogenetics: 1 E4
• Gene Summary: Nuclear receptor that acts as a key metabolic sensor by regulating the expression of genes involved in bile acid synthesis, cholesterol homeostasis and triglyceride synthesis. Together with the oxysterol receptors NR1H3/LXR-alpha and NR1H2/LXR-beta, acts as an essential transcriptional regulator of lipid metabolism. Plays an anti-inflammatory role during the hepatic acute phase response by acting as a corepressor: inhibits the hepatic acute phase response by preventing dissociation of the N-Cor corepressor complex. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development (By similarity). Activates the transcription of CYP2C38 (PubMed:30555544).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region compared to variant 1. The resulting isoform (2) contains a distinct and shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.