Ch25h (NM_009890) Mouse Tagged ORF Clone
CAT#: MR225185
- TrueORF®
Ch25h (Myc-DDK-tagged) - Mouse cholesterol 25-hydroxylase (Ch25h)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_009890" in other vectors (4)
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USD 198.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Ch25h |
Synonyms | AI462618; m25OH |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR225185 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTGCTACAACGGTTCGGAGCTCCAAGACCTGGGCTGTTCCAGCCAGCTGCTCCTGCAGCCCCTCT GGGACACCATAAGGACAAGGGAGGCGTTCACGCGCTCACCCATCTTCCCAGTCACCTTTTCTATCATCAC TTACGTGGGCTTCTGCCTACCGTTCGTGGTGCTGGACGTCCTGTATCCCTGGGTCCCCATCCTGCGACGC TACAAGATCCACCCGGACTTCTCGCCTTCCGTAAAGCAGCTTCTGCCTTGCCTGGGGCTGACACTCTACC AGCACCTGGTGTTCGTGTTCCCGGTGACGCTGCTGCACTGGGTGCGCAGCCCGGCGCTCCTCCCCCAGGA GGCCCCTGAGCTCGTCCAGCTCCTAAGTCACGTCCTGATCTGCCTGCTGCTCTTCGACACCGAGATCTTC GCGTGGCACCTGCTGCACCATAAGGTGCCCTGGCTGTACCGCACCTTCCACAAGGTGCATCACCAGAACT CGTCCTCCTTCGCGCTGGCGACCCAATACATGAGCTTCTGGGAGCTGCTTTCGCTGACCTTCTTCGACGT GCTGAACGTCGCGGTGCTTCGGTGTCACCCACTCACCATCTTTACCTTTCACGTGATTAACATCTGGCTG TCGGTGGAGGACCACTCGGGCTATGACTTCCCGTGGTCCACTCACAGACTTGTGCCCTTTGGCTGGTACG GGGGCGTGGCTCACCACGACATGCATCACTCTCAGTTTAACTGCAATTTTGCTCCTTACTTCACACACTG GGACAAAATGCTGGGCACTCTGCGGTCCGCGCCCCTGCCAGAGAGCCTTTGCGCCTGCGGTGAGCGCTGC GTGAACTCCAGAGAGCGATGCGCTGTACACTTGATCCAGAAGAAGAAACAGACT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225185 protein sequence
Red=Cloning site Green=Tags(s) MGCYNGSELQDLGCSSQLLLQPLWDTIRTREAFTRSPIFPVTFSIITYVGFCLPFVVLDVLYPWVPILRR YKIHPDFSPSVKQLLPCLGLTLYQHLVFVFPVTLLHWVRSPALLPQEAPELVQLLSHVLICLLLFDTEIF AWHLLHHKVPWLYRTFHKVHHQNSSSFALATQYMSFWELLSLTFFDVLNVAVLRCHPLTIFTFHVINIWL SVEDHSGYDFPWSTHRLVPFGWYGGVAHHDMHHSQFNCNFAPYFTHWDKMLGTLRSAPLPESLCACGERC VNSRERCAVHLIQKKKQT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_009890 |
ORF Size | 897 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_009890.2 |
RefSeq Size | 1366 bp |
RefSeq ORF | 897 bp |
Locus ID | 12642 |
UniProt ID | Q9Z0F5 |
Cytogenetics | 19 C1 |
MW | 34.7 kDa |
Gene Summary | Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes (PubMed:9852097). Plays a key role in cell positioning and movement in lymphoid tissues: 25-hydroxycholesterol is an intermediate in biosynthesis of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC), an oxysterol that acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells (PubMed:22999953). May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing (PubMed:9852097). In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation (PubMed:9852097).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC208281 | Ch25h (untagged) - Mouse cholesterol 25-hydroxylase (Ch25h), (10ug) |
USD 330.00 |
|
MG225185 | Ch25h (tGFP-tagged) - Mouse cholesterol 25-hydroxylase (Ch25h), (10ug) |
USD 500.00 |
|
MR225185L3 | Lenti ORF clone of Ch25h (Myc-DDK-tagged) - Mouse cholesterol 25-hydroxylase (Ch25h) |
USD 600.00 |
|
MR225185L4 | Lenti ORF clone of Ch25h (mGFP-tagged) - Mouse cholesterol 25-hydroxylase (Ch25h) |
USD 600.00 |
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