Ch25h (NM_009890) Mouse Untagged Clone

CAT#: MC208281

Ch25h (untagged) - Mouse cholesterol 25-hydroxylase (Ch25h), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ch25h
• Synonyms: AI462618; m25OH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208281 representing NM_009890
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTGCTACAACGGTTCGGAGCTCCAAGACCTGGGCTGTTCCAGCCAGCTGCTCCTGCAGCCCCTCT
  GGGACACCATAAGGACAAGGGAGGCGTTCACGCGCTCACCCATCTTCCCAGTCACCTTTTCTATCATCAC
  TTACGTGGGCTTCTGCCTACCGTTCGTGGTGCTGGACGTCCTGTATCCCTGGGTCCCCATCCTGCGACGC
  TACAAGATCCACCCGGACTTCTCGCCTTCCGTAAAGCAGCTTCTGCCTTGCCTGGGGCTGACACTCTACC
  AGCACCTGGTGTTCGTGTTCCCGGTGACGCTGCTGCACTGGGTGCGCAGCCCGGCGCTCCTCCCCCAGGA
  GGCCCCTGAGCTCGTCCAGCTCCTAAGTCACGTCCTGATCTGCCTGCTGCTCTTCGACACCGAGATCTTC
  GCGTGGCACCTGCTGCACCATAAGGTGCCCTGGCTGTACCGCACCTTCCACAAGGTGCATCACCAGAACT
  CGTCCTCCTTCGCGCTGGCGACCCAATACATGAGCTTCTGGGAGCTGCTTTCGCTGACCTTCTTCGACGT
  GCTGAACGTCGCGGTGCTTCGGTGTCACCCACTCACCATCTTTACCTTTCACGTGATTAACATCTGGCTG
  TCGGTGGAGGACCACTCGGGCTATGACTTCCCGTGGTCCACTCACAGACTTGTGCCCTTTGGCTGGTACG
  GGGGCGTGGCTCACCACGACATGCATCACTCTCAGTTTAACTGCAATTTTGCTCCTTACTTCACACACTG
  GGACAAAATGCTGGGCACTCTGCGGTCCGCGCCCCTGCCAGAGAGCCTTTGCGCCTGCGGTGAGCGCTGC
  GTGAACTCCAGAGAGCGATGCGCTGTACACTTGATCCAGAAGAAGAAACAGACTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009890
• Insert Size: 897 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009890.1, NP_034020.1
• RefSeq Size: 1365 bp
• RefSeq ORF: 897 bp
• Locus ID: 12642
• UniProt ID: Q9Z0F5
• Cytogenetics: 19 C1
• Gene Summary: Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes (PubMed:9852097). Plays a key role in cell positioning and movement in lymphoid tissues: 25-hydroxycholesterol is an intermediate in biosynthesis of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC), an oxysterol that acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells (PubMed:22999953). May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing (PubMed:9852097). In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation (PubMed:9852097).[UniProtKB/Swiss-Prot Function]