Adcy3 (NM_138305) Mouse Tagged ORF Clone

CAT#: MR224967

Adcy3 (Myc-DDK-tagged) - Mouse adenylate cyclase 3 (Adcy3), transcript variant 1

Product Images

Circular map for MR224967

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Adcy3
• Synonyms: AC3; mKIAA0511
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224967 representing NM_138305.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCCGAGGAACCAGGGCTTCTCGGATCCCGAGTACTCGGCAGAGTACTCAGCTGAATACTCGGTCAGC
  CTGCCCTCTGACCCCGACCGCGGGGTCGGCCGGACCCATGAAATTTCTGTGCGGAACTCGGGGTCCTGC
  CTGTGCCTGCCTCGCTTTATGCGGCTGACCTTCGTGCCTGAGTCCTTGGAGAACCTCTACCAGACCTAC
  TTCAAAAGGCAGCGCCATGAGACCCTGCTGGTGCTGGTGGTCTTTGCGGCCCTCTTTGACTGCTACGTG
  GTAGTGATGTGCGCGGTGGTCTTCTCCAGCGACAAGCTGGCGCCCCTCATGGTGGCAGGCTTCGGTCTG
  GTGTTGGACATCATCCTTTTCGTGCTCTGTAAAAAGGGGCTGCTCCCGGACCGAGTGAGCCGCAAAGTG
  GTACCCTACCTGCTGTGGCTGCTCATCTCGGCCCAGATCTTCTCCTACCTGGGCCTGAACTTTTCACGG
  GCCCACGCAGCCAGTGACACTGTGGGTTGGCAGGCTTTCTTTGTCTTCTCCTTCTTCATAACGCTGCCC
  CTCAGCCTCAGCCCCATCGTGATCATCTCTGTGGTCTCCTGTGTTGTGCACACGCTTGTGTTGGGGGTC
  ACTGTGGCCCAGCAGCAGCAGGATGAGCTGGAAGGGATGCAGCTGCTGAGGGAGATCCTGGCCAACGTC
  TTCCTCTACCTGTGTGCTATCATCGTGGGCATCATGTCCTACTACATGGCAGACCGCAAGCACCGCAAG
  GCCTTCCTGGAGGCCCGCCAGTCACTGGAGGTGAAGATGAATCTGGAGGAGCAGAGCCAGCAGCAGGAA
  AACCTTATGCTTTCCATCCTGCCCAAGCACGTGGCTGACGAGATGCTGAAGGACATGAAGAAGGACGAG
  AGCCAGAAGGACCAGCAGCAGTTCAATACCATGTACATGTACCGGCACGAGAATGTCAGCATCCTGTTT
  GCAGATATTGTGGGCTTTACCCAGCTGTCCTCTGCTTGCAGTGCCCAGGAGCTCGTGAAGTTACTCAAC
  GAGCTCTTCGCCCGCTTTGACAAGCTGGCAGCCAAATACCACCAGCTGAGGATCAAGATCCTAGGCGAC
  TGTTACTACTGCATCTGCGGCTTGCCTGACTACCGGGAGGACCATGCCGTGTGCTCCATCCTTATGGGG
  CTTGCCATGGTAGAGGCCATCTCGTACGTGCGGGAGAAGACCAAGACTGGAGTGGACATGCGTGTGGGA
  GTGCACACAGGCACTGTCCTAGGCGGTGTCCTGGGCCAGAAGCGCTGGCAGTATGATGTATGGTCTACT
  GATGTCACCGTGGCAAACAAGATGGAGGCTGGTGGCATCCCAGGGCGCGTGCACATTTCCCAGAGCACC
  ATGGACTGCCTGAAGGGGGAGTTTGATGTGGAGTCTGGTGATGGGGGCAGTCGCTGCGACTACCTAGAT
  GAGAAGGGCATCGAAACCTACCTCATCATTGCCTCCAAGCCAGAGGTGAAGAAAACAGCCCAAAATGGC
  CTCAACGGCTCGGCCGTGCCAAACGGAGCGCCGGCATCCTCCAAACCCAGCTCCCCTGCCCTCATCGAG
  ACCAAGGAGCCCAATGGAAGTGCCCATGCCAGCGGCTCCACATCAGAGGAGGCTGAAGAACAGGAGGCC
  CAGGCTGACAACCCCTCGTTCCCCAACCCCCGCCGCAGGCTGCGCCTCCAGGACCTGGCGGACCGTGTG
  GTGGACGCCTCTGAGGATGAGCACGAACTGAACCAGCTGCTTAATGAGGCCCTGCTGGAGCGGGAGTCC
  GCCCAGGTGGTAAAGAAGAGAAACACATTCCTCCTGACCATGAGGTTCATGGACCCAGAGATGGAAACA
  CGCTACTCGGTGGAGAAGGAGAAGCAGAGCGGGGCTGCCTTCAGCTGCTCCTGTGTGGTCCTCTTCTGC
  ACGGCCATGGTGGAGATACTCATTGACCCCTGGCTGATGACAAACTATGTGACCTTTGTGGTTGGGGAG
  GTTCTGCTCCTGATCCTGACCATCTGCTCGATGGCTGCCATCTTTCCCAGGTCATTTCCCAAGAAGCTT
  GTGGCCTTCTCATCTTGGATTGACCGGACCCGCTGGGCAAGGAACACCTGGGCCATGTTAGCCATCTTC
  ATTCTGGTTATGGCCAATGTTGTGGACATGCTCAGCTGTCTCCAGTACTACATGGGACCTTACAACATG
  ACAGCTGGGATGGAGCTGGACGGCGGCTGCATGGAGAACCCCAAGTACTACAACTACGTAGCTGTGCTG
  TCGCTCATCGCCACCATCATGCTGGTGCAGGTCAGCCACATGGTGAAGCTGACGCTCATGCTGCTCGTC
  ACAGGCGCCGTGACTGCCCTCAACCTGTACGCCTGGTGTCCTGTCTTTGATGAATACGACCACAAGCGC
  TTTCAGGAAAAGGACTCTCCTATGGTGGCCTTAGAGAAGATGCAGGTACTTGCCACCCCTGGGCTCAAT
  GGCACTGACAGGCTGCCCCTGGTGCCTTCCAAGTACTCCATGACTGTGATGATGTTCGTCATGATGCTG
  AGCTTTTACTACTTCTCGCGCCACGTGGAAAAACTGGCCCGAACACTGTTCTTGTGGAAGATTGAGGTC
  CATGACCAGAAAGAACGTGTCTACGAGATGCGCCGATGGAACGAGGCCTTGGTCACCAACATGTTGCCT
  GAGCATGTTGCACGCCATTTTCTGGGGTCCAAGAAGAGAGATGAGGAGCTGTACAGCCAGTCTTATGAC
  GAGATTGGAGTCATGTTTGCCTCCTTGCCCAACTTTGCTGACTTCTACACTGAGGAGAGCATCAACAAC
  GGCGGCATCGAGTGTCTACGCTTCCTCAATGAGATCATCTCTGATTTTGACTCTCTCCTGGACAATCCC
  AAATTCCGGGTCATCACCAAGATCAAAACTATTGGCAGCACCTATATGGCAGCTTCAGGAGTCACACCA
  GATGTCAACACCAATGGCTTTACAAGCTCCAGCAAGGAGGAGAAGTCAGACAAGGAGCGCTGGCAGCAC
  CTGGCTGACCTGGCTGACTTTGCATTAGCCATGAAGGACACGCTCACAAACATCAACAACCAGTCATTC
  AACAACTTCATGTTGCGCATAGGCATGAACAAAGGAGGGGTTCTGGCTGGCGTCATTGGAGCCCGGAAA
  CCACACTATGACATCTGGGGCAATACGGTCAATGTGGCCAGCAGGATGGAATCCACAGGAGTCATGGGC
  AACATCCAGGTGGTAGAAGAGACGCAGGTCATCCTTCGAGAGTACGGCTTCCGCTTTGTGAGGCGAGGT
  CCCATCTTTGTGAAAGGCAAAGGGGAGCTTCTGACCTTTTTCTTGAAGGGGCGGGACAGGCCAGCTGCC
  TTCCCCAATGGCTCCTCTGTTACACTGCCCCACCAAGTGGTGGACAACCCC
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
  Protein Sequence

  >Peptide sequence encoded by MR224967
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MPRNQGFSDPEYSAEYSAEYSVSLPSDPDRGVGRTHEISVRNSGSCLCLPRFMRLTFVPESLENLYQTY
  FKRQRHETLLVLVVFAALFDCYVVVMCAVVFSSDKLAPLMVAGFGLVLDIILFVLCKKGLLPDRVSRKV
  VPYLLWLLISAQIFSYLGLNFSRAHAASDTVGWQAFFVFSFFITLPLSLSPIVIISVVSCVVHTLVLGV
  TVAQQQQDELEGMQLLREILANVFLYLCAIIVGIMSYYMADRKHRKAFLEARQSLEVKMNLEEQSQQQE
  NLMLSILPKHVADEMLKDMKKDESQKDQQQFNTMYMYRHENVSILFADIVGFTQLSSACSAQELVKLLN
  ELFARFDKLAAKYHQLRIKILGDCYYCICGLPDYREDHAVCSILMGLAMVEAISYVREKTKTGVDMRVG
  VHTGTVLGGVLGQKRWQYDVWSTDVTVANKMEAGGIPGRVHISQSTMDCLKGEFDVESGDGGSRCDYLD
  EKGIETYLIIASKPEVKKTAQNGLNGSAVPNGAPASSKPSSPALIETKEPNGSAHASGSTSEEAEEQEA
  QADNPSFPNPRRRLRLQDLADRVVDASEDEHELNQLLNEALLERESAQVVKKRNTFLLTMRFMDPEMET
  RYSVEKEKQSGAAFSCSCVVLFCTAMVEILIDPWLMTNYVTFVVGEVLLLILTICSMAAIFPRSFPKKL
  VAFSSWIDRTRWARNTWAMLAIFILVMANVVDMLSCLQYYMGPYNMTAGMELDGGCMENPKYYNYVAVL
  SLIATIMLVQVSHMVKLTLMLLVTGAVTALNLYAWCPVFDEYDHKRFQEKDSPMVALEKMQVLATPGLN
  GTDRLPLVPSKYSMTVMMFVMMLSFYYFSRHVEKLARTLFLWKIEVHDQKERVYEMRRWNEALVTNMLP
  EHVARHFLGSKKRDEELYSQSYDEIGVMFASLPNFADFYTEESINNGGIECLRFLNEIISDFDSLLDNP
  KFRVITKIKTIGSTYMAASGVTPDVNTNGFTSSSKEEKSDKERWQHLADLADFALAMKDTLTNINNQSF
  NNFMLRIGMNKGGVLAGVIGARKPHYDIWGNTVNVASRMESTGVMGNIQVVEETQVILREYGFRFVRRG
  PIFVKGKGELLTFFLKGRDRPAAFPNGSSVTLPHQVVDNP
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138305
• ORF Size: 3432 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 4348 bp
• RefSeq ORF: 3438 bp
• Locus ID: 104111
• UniProt ID: Q8VHH7
• Cytogenetics: 12 A1.1
• MW: 129 kDa
• Gene Summary: Catalyzes the formation of the signaling molecule cAMP in response to G-protein signaling (PubMed:9768837, PubMed:11055432, PubMed:25329148). Participates in signaling cascades triggered by odorant receptors via its function in cAMP biosynthesis (PubMed:9768837, PubMed:11055432). Required for the perception of odorants (PubMed:11055432). Required for normal sperm motility and normal male fertility (PubMed:15705663). Plays a role in regulating insulin levels and body fat accumulation in response to a high fat diet (PubMed:25329148).[UniProtKB/Swiss-Prot Function]