Adcy3 (NM_138305) Mouse Tagged ORF Clone

CAT#: MG224967

Adcy3 (tGFP-tagged) - Mouse adenylate cyclase 3 (Adcy3) transcript variant 1, (10ug)

Product Images

Circular map for MG224967

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Adcy3
• Synonyms: AC3; mKIAA0511
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224967 representing NM_138305
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGAGGAACCAGGGCTTCTCGGATCCCGAGTACTCGGCAGAGTACTCAGCTGAATACTCGGTCAGCC
  TGCCCTCTGACCCCGACCGCGGGGTCGGCCGGACCCATGAAATTTCTGTGCGGAACTCGGGGTCCTGCCT
  GTGCCTGCCTCGCTTTATGCGGCTGACCTTCGTGCCTGAGTCCTTGGAGAACCTCTACCAGACCTACTTC
  AAAAGGCAGCGCCATGAGACCCTGCTGGTGCTGGTGGTCTTTGCGGCCCTCTTTGACTGCTACGTGGTAG
  TGATGTGCGCGGTGGTCTTCTCCAGCGACAAGCTGGCGCCCCTCATGGTGGCAGGCTTCGGTCTGGTGTT
  GGACATCATCCTTTTCGTGCTCTGTAAAAAGGGGCTGCTCCCGGACCGAGTGAGCCGCAAAGTGGTACCC
  TACCTGCTGTGGCTGCTCATCTCGGCCCAGATCTTCTCCTACCTGGGCCTGAACTTTTCACGGGCCCACG
  CAGCCAGTGACACTGTGGGTTGGCAGGCTTTCTTTGTCTTCTCCTTCTTCATAACGCTGCCCCTCAGCCT
  CAGCCCCATCGTGATCATCTCTGTGGTCTCCTGTGTTGTGCACACGCTTGTGTTGGGGGTCACTGTGGCC
  CAGCAGCAGCAGGATGAGCTGGAAGGGATGCAGCTGCTGAGGGAGATCCTGGCCAACGTCTTCCTCTACC
  TGTGTGCTATCATCGTGGGCATCATGTCCTACTACATGGCAGACCGCAAGCACCGCAAGGCCTTCCTGGA
  GGCCCGCCAGTCACTGGAGGTGAAGATGAATCTGGAGGAGCAGAGCCAGCAGCAGGAAAACCTTATGCTT
  TCCATCCTGCCCAAGCACGTGGCTGACGAGATGCTGAAGGACATGAAGAAGGACGAGAGCCAGAAGGACC
  AGCAGCAGTTCAATACCATGTACATGTACCGGCACGAGAATGTCAGCATCCTGTTTGCAGATATTGTGGG
  CTTTACCCAGCTGTCCTCTGCTTGCAGTGCCCAGGAGCTCGTGAAGTTACTCAACGAGCTCTTCGCCCGC
  TTTGACAAGCTGGCAGCCAAATACCACCAGCTGAGGATCAAGATCCTAGGCGACTGTTACTACTGCATCT
  GCGGCTTGCCTGACTACCGGGAGGACCATGCCGTGTGCTCCATCCTTATGGGGCTTGCCATGGTAGAGGC
  CATCTCGTACGTGCGGGAGAAGACCAAGACTGGAGTGGACATGCGTGTGGGAGTGCACACAGGCACTGTC
  CTAGGCGGTGTCCTGGGCCAGAAGCGCTGGCAGTATGATGTATGGTCTACTGATGTCACCGTGGCAAACA
  AGATGGAGGCTGGTGGCATCCCAGGGCGCGTGCACATTTCCCAGAGCACCATGGACTGCCTGAAGGGGGA
  GTTTGATGTGGAGTCTGGTGATGGGGGCAGTCGCTGCGACTACCTAGATGAGAAGGGCATCGAAACCTAC
  CTCATCATTGCCTCCAAGCCAGAGGTGAAGAAAACAGCCCAAAATGGCCTCAACGGCTCGGCCGTGCCAA
  ACGGAGCGCCGGCATCCTCCAAACCCAGCTCCCCTGCCCTCATCGAGACCAAGGAGCCCAATGGAAGTGC
  CCATGCCAGCGGCTCCACATCAGAGGAGGCTGAAGAACAGGAGGCCCAGGCTGACAACCCCTCGTTCCCC
  AACCCCCGCCGCAGGCTGCGCCTCCAGGACCTGGCGGACCGTGTGGTGGACGCCTCTGAGGATGAGCACG
  AACTGAACCAGCTGCTTAATGAGGCCCTGCTGGAGCGGGAGTCCGCCCAGGTGGTAAAGAAGAGAAACAC
  ATTCCTCCTGACCATGAGGTTCATGGACCCAGAGATGGAAACACGCTACTCGGTGGAGAAGGAGAAGCAG
  AGCGGGGCTGCCTTCAGCTGCTCCTGTGTGGTCCTCTTCTGCACGGCCATGGTGGAGATACTCATTGACC
  CCTGGCTGATGACAAACTATGTGACCTTTGTGGTTGGGGAGGTTCTGCTCCTGATCCTGACCATCTGCTC
  GATGGCTGCCATCTTTCCCAGGTCATTTCCCAAGAAGCTTGTGGCCTTCTCATCTTGGATTGACCGGACC
  CGCTGGGCAAGGAACACCTGGGCCATGTTAGCCATCTTCATTCTGGTTATGGCCAATGTTGTGGACATGC
  TCAGCTGTCTCCAGTACTACATGGGACCTTACAACATGACAGCTGGGATGGAGCTGGACGGCGGCTGCAT
  GGAGAACCCCAAGTACTACAACTACGTAGCTGTGCTGTCGCTCATCGCCACCATCATGCTGGTGCAGGTC
  AGCCACATGGTGAAGCTGACGCTCATGCTGCTCGTCACAGGCGCCGTGACTGCCCTCAACCTGTACGCCT
  GGTGTCCTGTCTTTGATGAATACGACCACAAGCGCTTTCAGGAAAAGGACTCTCCTATGGTGGCCTTAGA
  GAAGATGCAGGTACTTGCCACCCCTGGGCTCAATGGCACTGACAGGCTGCCCCTGGTGCCTTCCAAGTAC
  TCCATGACTGTGATGATGTTCGTCATGATGCTGAGCTTTTACTACTTCTCGCGCCACGTGGAAAAACTGG
  CCCGAACACTGTTCTTGTGGAAGATTGAGGTCCATGACCAGAAAGAACGTGTCTACGAGATGCGCCGATG
  GAACGAGGCCTTGGTCACCAACATGTTGCCTGAGCATGTTGCACGCCATTTTCTGGGGTCCAAGAAGAGA
  GATGAGGAGCTGTACAGCCAGTCTTATGACGAGATTGGAGTCATGTTTGCCTCCTTGCCCAACTTTGCTG
  ACTTCTACACTGAGGAGAGCATCAACAACGGCGGCATCGAGTGTCTACGCTTCCTCAATGAGATCATCTC
  TGATTTTGACTCTCTCCTGGACAATCCCAAATTCCGGGTCATCACCAAGATCAAAACTATTGGCAGCACC
  TATATGGCAGCTTCAGGAGTCACACCAGATGTCAACACCAATGGCTTTACAAGCTCCAGCAAGGAGGAGA
  AGTCAGACAAGGAGCGCTGGCAGCACCTGGCTGACCTGGCTGACTTTGCATTAGCCATGAAGGACACGCT
  CACAAACATCAACAACCAGTCATTCAACAACTTCATGTTGCGCATAGGCATGAACAAAGGAGGGGTTCTG
  GCTGGCGTCATTGGAGCCCGGAAACCACACTATGACATCTGGGGCAATACGGTCAATGTGGCCAGCAGGA
  TGGAATCCACAGGAGTCATGGGCAACATCCAGGTGGTAGAAGAGACGCAGGTCATCCTTCGAGAGTACGG
  CTTCCGCTTTGTGAGGCGAGGTCCCATCTTTGTGAAAGGCAAAGGGGAGCTTCTGACCTTTTTCTTGAAG
  GGGCGGGACAGGCCAGCTGCCTTCCCCAATGGCTCCTCTGTTACACTGCCCCACCAAGTGGTGGACAACC
  CC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224967 representing NM_138305
  Red=Cloning site Green=Tags(s)
  
  MPRNQGFSDPEYSAEYSAEYSVSLPSDPDRGVGRTHEISVRNSGSCLCLPRFMRLTFVPESLENLYQTYF
  KRQRHETLLVLVVFAALFDCYVVVMCAVVFSSDKLAPLMVAGFGLVLDIILFVLCKKGLLPDRVSRKVVP
  YLLWLLISAQIFSYLGLNFSRAHAASDTVGWQAFFVFSFFITLPLSLSPIVIISVVSCVVHTLVLGVTVA
  QQQQDELEGMQLLREILANVFLYLCAIIVGIMSYYMADRKHRKAFLEARQSLEVKMNLEEQSQQQENLML
  SILPKHVADEMLKDMKKDESQKDQQQFNTMYMYRHENVSILFADIVGFTQLSSACSAQELVKLLNELFAR
  FDKLAAKYHQLRIKILGDCYYCICGLPDYREDHAVCSILMGLAMVEAISYVREKTKTGVDMRVGVHTGTV
  LGGVLGQKRWQYDVWSTDVTVANKMEAGGIPGRVHISQSTMDCLKGEFDVESGDGGSRCDYLDEKGIETY
  LIIASKPEVKKTAQNGLNGSAVPNGAPASSKPSSPALIETKEPNGSAHASGSTSEEAEEQEAQADNPSFP
  NPRRRLRLQDLADRVVDASEDEHELNQLLNEALLERESAQVVKKRNTFLLTMRFMDPEMETRYSVEKEKQ
  SGAAFSCSCVVLFCTAMVEILIDPWLMTNYVTFVVGEVLLLILTICSMAAIFPRSFPKKLVAFSSWIDRT
  RWARNTWAMLAIFILVMANVVDMLSCLQYYMGPYNMTAGMELDGGCMENPKYYNYVAVLSLIATIMLVQV
  SHMVKLTLMLLVTGAVTALNLYAWCPVFDEYDHKRFQEKDSPMVALEKMQVLATPGLNGTDRLPLVPSKY
  SMTVMMFVMMLSFYYFSRHVEKLARTLFLWKIEVHDQKERVYEMRRWNEALVTNMLPEHVARHFLGSKKR
  DEELYSQSYDEIGVMFASLPNFADFYTEESINNGGIECLRFLNEIISDFDSLLDNPKFRVITKIKTIGST
  YMAASGVTPDVNTNGFTSSSKEEKSDKERWQHLADLADFALAMKDTLTNINNQSFNNFMLRIGMNKGGVL
  AGVIGARKPHYDIWGNTVNVASRMESTGVMGNIQVVEETQVILREYGFRFVRRGPIFVKGKGELLTFFLK
  GRDRPAAFPNGSSVTLPHQVVDNP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138305
• ORF Size: 3435 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138305.3, NP_612178.2
• RefSeq Size: 4348 bp
• RefSeq ORF: 3438 bp
• Locus ID: 104111
• UniProt ID: Q8VHH7
• Cytogenetics: 12 A1.1
• Gene Summary: Catalyzes the formation of the signaling molecule cAMP in response to G-protein signaling (PubMed:9768837, PubMed:11055432, PubMed:25329148). Participates in signaling cascades triggered by odorant receptors via its function in cAMP biosynthesis (PubMed:9768837, PubMed:11055432). Required for the perception of odorants (PubMed:11055432). Required for normal sperm motility and normal male fertility (PubMed:15705663). Plays a role in regulating insulin levels and body fat accumulation in response to a high fat diet (PubMed:25329148).[UniProtKB/Swiss-Prot Function]