Hsd17b3 (NM_008291) Mouse Tagged ORF Clone

CAT#: MR224793

Hsd17b3 (Myc-DDK-tagged) - Mouse hydroxysteroid (17-beta) dehydrogenase 3 (Hsd17b3)

Product Images

Circular map for MR224793

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hsd17b3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224793 representing NM_008291
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAAGCTCTTCATTGCTGCAGGGCTCTTCGTGGGCCTGGTTTGCCTGGTGAAGTGCATGAGGTTCT
  CGCAGCACCTTTTTCTGAGGTTCTGCAAGGCTTTGCCAAGCTCTTTCCTGCGATCAATGGGACAATGGGC
  AGTGATTACCGGAGCAGGCGATGGCATCGGGAAAGCCTATTCATTTGAGTTGGCCAGACATGGACTCAAT
  GTTGTACTTATTAGTCGGACACTGGAAAAGCTACAGACCATTGCAGAAGAGATTGAGAGGACCACTGGAA
  GCTGTGTGAAGATTGTACAAGCAGATTTTACCAGAGAAGACATCTATGACCATATTAAAGAACACCTTGA
  AGGCTTAGAAATAGGAATTTTAGTCAACAACGTTGGAATGCTCCCCAGCTTTTTCCCAAGCCATTTCCTG
  AGCACTTCCGGTGAGAGCCAGAATCTCATCCACTGCAACATTACCTCCGTAGTCAAGATGACACAGCTTG
  TTCTCAAACACATGGAGTCAAGGAGGAAAGGCCTCATCTTGAATATTTCTTCAGGCGCAGCCCTTCGTCC
  CTGGCCTCTTTACAGCCTGTACTCAGCTTCCAAGGCTTTTGTGTACACATTTTCCAAGGCTCTGAGTGTG
  GAATACAGAGATAAAGGAATCATTATTCAGGTGCTGACCCCTTATTCTATTTCAACCCCAATGACAAAGT
  ACCTAAATAACAAGATGACCAAGACCGCCGATGAGTTTGTTAAAGAATCCTTGAAATATGTCACGATCGG
  AGCTGAATCCTGTGGCTGCCTTGCTCATGAAATCATTGCAATAATTCTGAACCGGATCCCTTCCAGAATC
  TTCTATAGCAGCACCGCTCAACGATTCCTCCTGACACGATACTCAGATTACCTCAAACGCAACATCAGCA
  ACAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224793 representing NM_008291
  Red=Cloning site Green=Tags(s)
  
  MEKLFIAAGLFVGLVCLVKCMRFSQHLFLRFCKALPSSFLRSMGQWAVITGAGDGIGKAYSFELARHGLN
  VVLISRTLEKLQTIAEEIERTTGSCVKIVQADFTREDIYDHIKEHLEGLEIGILVNNVGMLPSFFPSHFL
  STSGESQNLIHCNITSVVKMTQLVLKHMESRRKGLILNISSGAALRPWPLYSLYSASKAFVYTFSKALSV
  EYRDKGIIIQVLTPYSISTPMTKYLNNKMTKTADEFVKESLKYVTIGAESCGCLAHEIIAIILNRIPSRI
  FYSSTAQRFLLTRYSDYLKRNISNR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008291
• ORF Size: 915 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008291.3, NP_032317.2
• RefSeq Size: 1286 bp
• RefSeq ORF: 918 bp
• Locus ID: 15487
• UniProt ID: P70385
• Cytogenetics: 13 B3
• MW: 34.8 kDa
• Gene Summary: Favors the reduction of androstenedione to testosterone. Uses NADPH while the two other EDH17B enzymes use NADH.[UniProtKB/Swiss-Prot Function]