Hsd17b3 (NM_008291) Mouse Untagged Clone

CAT#: MC208712

Hsd17b3 (untagged) - Mouse hydroxysteroid (17-beta) dehydrogenase 3 (Hsd17b3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hsd17b3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208712 representing NM_008291
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAAGCTCTTCATTGCTGCAGGGCTCTTCGTGGGCCTGGTTTGCCTGGTGAAGTGCATGAGGTTCT
  CGCAGCACCTTTTTCTGAGGTTCTGCAAGGCTTTGCCAAGCTCTTTCCTGCGATCAATGGGACAATGGGC
  AGTGATTACCGGAGCAGGCGATGGCATCGGGAAAGCCTATTCATTTGAGTTGGCCAGACATGGACTCAAT
  GTTGTACTTATTAGTCGGACACTGGAAAAGCTACAGACCATTGCAGAAGAGATTGAGAGGACCACTGGAA
  GCTGTGTGAAGATTGTACAAGCAGATTTTACCAGAGAAGACATCTATGACCATATTAAAGAACACCTTGA
  AGGCTTAGAAATAGGAATTTTAGTCAACAACGTTGGAATGCTCCCCAGCTTTTTCCCAAGCCATTTCCTG
  AGCACTTCCGGTGAGAGCCAGAATCTCATCCACTGCAACATTACCTCCGTAGTCAAGATGACACAGCTTG
  TTCTCAAACACATGGAGTCAAGGAGGAAAGGCCTCATCTTGAATATTTCTTCAGGCGCAGCCCTTCGTCC
  CTGGCCTCTTTACAGCCTGTACTCAGCTTCCAAGGCTTTTGTGTACACATTTTCCAAGGCTCTGAGTGTG
  GAATACAGAGATAAAGGAATCATTATTCAGGTGCTGACCCCTTATTCTATTTCAACCCCAATGACAAAGT
  ACCTAAATAACAAGATGACCAAGACCGCCGATGAGTTTGTTAAAGAATCCTTGAAATATGTCACGATCGG
  AGCTGAATCCTGTGGCTGCCTTGCTCATGAAATCATTGCAATAATTCTGAACCGGATCCCTTCCAGAATC
  TTCTATAGCAGCACCGCTCAACGATTCCTCCTGACACGATACTCAGATTACCTCAAACGCAACATCAGCA
  ACAGATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008291
• Insert Size: 918 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008291.3, NP_032317.2
• RefSeq Size: 1286 bp
• RefSeq ORF: 918 bp
• Locus ID: 15487
• UniProt ID: P70385
• Cytogenetics: 13 B3
• Gene Summary: Favors the reduction of androstenedione to testosterone. Uses NADPH while the two other EDH17B enzymes use NADH.[UniProtKB/Swiss-Prot Function]