Nlrp6 (NM_001081389) Mouse Tagged ORF Clone

CAT#: MR224259

Nlrp6 (Myc-DDK-tagged) - Mouse NLR family, pyrin domain containing 6 (Nlrp6)

Product Images

Circular map for MR224259

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nlrp6
• Synonyms: AI504961; Nalp6; Navr/Avr; Pypaf5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224259 representing NM_001081389
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGCCGCTGGAGCCTCCTGCTCCAGCGTGGATGCAGTGGCAAGGGAGCTGCTTATGGCTACACTGG
  AGGAGCTGAGCCAGGAGCAGCTGAAGCGCTTCCGCCACAAGCTAAGGGATGCGCCCCTGGATGGCCGCAG
  CATCCCGTGGGGGCGACTGGAGCGCTCAGACGCTGTGGACCTTGTCGACAAGCTCATTGAGTTCTATGAG
  CCAGTGCCAGCTGTAGAAATGACCCGGCAGGTCCTGAAGAGGTCGGATATTCGAGATGTGGCCTCGCGAC
  TTAAGCAACAGCAGCTACAGAAGCTCGGCCCCACCTCGGTGCTTCTCTCCGTGTCAGCGTTCAAGAAGAA
  GTACCGCGAGCACGTGCTACGACAGCATGCTAAGGTGAAGGAGAGGAATGCTCGCTCTGTGAAGATCAAC
  AAGCGCTTCACCAAGCTGCTCATCGCTCCGGGGACCGGCGCTGTGGAGGACGAGCTGCTGGGGCCGTTGG
  GGGAGCCGGAGCCCGAACGCGCTCGGCGCTCGGACACGCACACTTTTAATCGGCTCTTCCGGGGAAATGA
  TGAAGAGAGCTCGCAGCCGCTGACCGTGGTGCTGCAGGGCCCGGCAGGCATCGGCAAAACCATGGCCGCC
  AAGAAAATCCTCTATGACTGGGCAGCAGGTAAGCTATACCACAGCCAGGTGGACTTTGCCTTCTTCATGC
  CGTGCGGCGAGCTGCTGGAGCGGCCGGGAAAGCGCAGCTTGGCGGACCTGGTGCTGGACCAGTGCCCGGA
  CCGCGCTTGGCCAGTGAAGCGGATACTGGCGCAACCCAATCGCCTACTGTTCATCCTGGACGGTGCAGAC
  GAGCTGCCTACTTTACCATCCTCAGAGGCCACTCCCTGCAAAGACCCTTTGGAGGCTACAAGTGGCTTGA
  GAGTGCTGAGCGGGCTGCTGAGCCAGGAGCTGTTACCAGGAGCTCGCTTGCTAGTGACTACACGCCATGC
  CGCTACAGGGAGGCTGCAGGGCAGATTGTGCTCGCCACAGTGCGCAGAAATACGCGGCTTCTCAGACAAA
  GACAAAAAAAAGTATTTCTTCAAGTTCTTCCGGGACGAGAGGAAGGCAGAGCGCGCTTACCGCTTCGTGA
  AAGAGAACGAGACGCTGTTTGCACTGTGCTTTGTGCCCTTCGTGTGCTGGATCGTGTGCACAGTGCTGCA
  GCAGCAGCTAGAGCTGGGCCGGGATCTCTCTCGTACCTCCAAGACCACTACTTCTGTGTACCTGCTCTTC
  ATCACCAGCATGCTGAAGTCTGCAGGCACCAATGGACCCCGGGTTCAGGGAGAGCTGAGAACGCTGTGTC
  GCCTGGCCAGGGAGGGCATCCTGGATCATCATAAAGCACAATTCTCAGAAGAGGACCTGGAGAAATTGAA
  GCTTCGGGGTTCCCAAGTTCAGACAATATTTCTCAACAAGAAAGAGATACCAGGAGTGCTAAAAACTGAG
  GTCACCTACCAGTTCATTGACCAGAGCTTCCAGGAGTTCTTGGCTGCATTGTCATACCTACTAGAAGCTG
  AGCGAACTCCGGGGACGCCCGCAGGCGGTGTGCAGAAGCTCCTGAACTCTGACGCGGAGCTACGTGGTCA
  TCTTGCACTGACCACCCGATTCCTCTTTGGCCTGCTAAATACAGAGGGGCTTCGTGACATTGGAAACCAT
  TTTGGCTGTGTGGTGCCAGATCATGTGAAAAAGGACACCCTGCGGTGGGTACAAGGACAGAGCCACCCCA
  AGGGGCCACCAGTAGGGGCAAAAAAGACTGCTGAGCTGGAGGACATCGAGGACGCAGAGGAGGAGGAGGA
  GGAAGAGGAGGACCTCAATTTTGGACTGGAGCTGCTGTACTGCCTGTATGAGACACAGGAGGAGGATTTT
  GTTCGACAGGCTCTCAGCAGCCTTCCAGAGATAGTACTGGAGCGAGTCAGGTTGACCCGCATGGACCTTG
  AAGTTCTGAATTACTGCGTGCAGTGCTGCCCAGATGGCCAGGCTCTGAGACTGGTGAGCTGTGGGCTGGT
  GGCGGCAAAGGAGAAGAAGAAGAAGAAGAAGAGCCTGGTGAAGCGGCTGAAGGGGAGCGTTACAGGCACC
  AACCTTGTTCTTTCTCTCTACAGCTCTCAAAGCACCAAGAAACAACCCCCAGTCTCCTTGCTGCGTCCAC
  TCTGTGAGACAATGACTACCCCGAAATGTCATCTGAGTGTTCTGATCTTGTCACACTGCAGACTCCCTGA
  TGCAGTTTGCCGAGACCTTTCCGAGGCCCTGAAGGTAGCTCCTGCCCTAAGGGAGTTGGGCCTCCTCCAG
  AGCCGGCTCACCAACACAGGCCTGCGTTTACTGTGCGAAGGCCTGGCTTGGCCCAAGTGCCAGGTGAAGA
  CACTCAGGATGCAGCTGCCTGACCTCCAAGAGGTGATCAATTACTTGGTCATCGTGCTCCAGCAGAGCCC
  AGTCCTGACCACTCTGGACCTCAGTGGCTGTCAGCTGCCTGGCGTCATTGTGGAACCTCTGTGTGCAGCC
  CTGAAGCACCCTAAATGCTCCCTAAAGACCCTCAGTCTGACCTCTGTGGAGCTGAGTGAGAACTCACTGA
  GGGATCTTCAAGCTGTGAAGACATCAAAGCCAGATCTGTCCATCATATATTCAAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224259 representing NM_001081389
  Red=Cloning site Green=Tags(s)
  
  MDAAGASCSSVDAVARELLMATLEELSQEQLKRFRHKLRDAPLDGRSIPWGRLERSDAVDLVDKLIEFYE
  PVPAVEMTRQVLKRSDIRDVASRLKQQQLQKLGPTSVLLSVSAFKKKYREHVLRQHAKVKERNARSVKIN
  KRFTKLLIAPGTGAVEDELLGPLGEPEPERARRSDTHTFNRLFRGNDEESSQPLTVVLQGPAGIGKTMAA
  KKILYDWAAGKLYHSQVDFAFFMPCGELLERPGKRSLADLVLDQCPDRAWPVKRILAQPNRLLFILDGAD
  ELPTLPSSEATPCKDPLEATSGLRVLSGLLSQELLPGARLLVTTRHAATGRLQGRLCSPQCAEIRGFSDK
  DKKKYFFKFFRDERKAERAYRFVKENETLFALCFVPFVCWIVCTVLQQQLELGRDLSRTSKTTTSVYLLF
  ITSMLKSAGTNGPRVQGELRTLCRLAREGILDHHKAQFSEEDLEKLKLRGSQVQTIFLNKKEIPGVLKTE
  VTYQFIDQSFQEFLAALSYLLEAERTPGTPAGGVQKLLNSDAELRGHLALTTRFLFGLLNTEGLRDIGNH
  FGCVVPDHVKKDTLRWVQGQSHPKGPPVGAKKTAELEDIEDAEEEEEEEEDLNFGLELLYCLYETQEEDF
  VRQALSSLPEIVLERVRLTRMDLEVLNYCVQCCPDGQALRLVSCGLVAAKEKKKKKKSLVKRLKGSVTGT
  NLVLSLYSSQSTKKQPPVSLLRPLCETMTTPKCHLSVLILSHCRLPDAVCRDLSEALKVAPALRELGLLQ
  SRLTNTGLRLLCEGLAWPKCQVKTLRMQLPDLQEVINYLVIVLQQSPVLTTLDLSGCQLPGVIVEPLCAA
  LKHPKCSLKTLSLTSVELSENSLRDLQAVKTSKPDLSIIYSK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001081389
• ORF Size: 2646 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081389.1, NP_001074858.1
• RefSeq Size: 3823 bp
• RefSeq ORF: 2649 bp
• Locus ID: 101613
• Cytogenetics: 7 F5
• MW: 140.1 kDa
• Gene Summary: The protein encoded by this gene binds arginine-vasopressin and may be involved in the arginine-vasopressin-mediated regulation of renal salt-water balance. The encoded protein also mediates inflammatory responses in the colon to allow recovery from intestinal epithelial damage and protects against tumorigenesis and the development of colitis. Finally, this protein can increase activation of NF-kappa-B, activation of CASP1 through interaction with ASC, and cAMP accumulation. [provided by RefSeq, Feb 2013]