Trim14 (NM_029077) Mouse Tagged ORF Clone

CAT#: MR224121

Trim14 (Myc-DDK-tagged) - Mouse tripartite motif-containing 14 (Trim14)

Product Images

Circular map for MR224121

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Trim14
• Synonyms: 5830400N10Rik; pub
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224121 representing NM_029077
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAGCGAGACTACCGAGGCGCGGGCGCCCTTTCAGCCCGACGGGGCGTATGGCTGGCGCTGTCCGG
  AGCACAGCGAACGCCCGGCTGAGCTCTTCTGTCGCCGCTGCGGCCGCTGTGTGTGCGCGCTCTGCCCGGT
  TCTGGGTGCGCACCGCGGCCACCCGGTGGGCCTGGCCGAGGAGGAGGCTGTGCGTGTGCAGAAGCTAATC
  CAGGACTGTTTAGAGTGTTTGGCAACCAAGAAGAGGCAACATGCGGACAACATAGCCCATTTGGAAGACG
  CCGGGGAAAGGCTCAAGGTTTACGCAGACTCAAGTAAAGCCTGGCTGACACAGAAATTCACAGAACTCAG
  ATTACTCCTGGATGAAGAGGAAGTACTGGCCAAAAAATTCATTGACAAAAGCACACAGCTCACCCTCCAG
  GTGTACCGGGAGCAAGCCGAGACTTGTGGGAAGCAGATTGAGGTCATGGATGACTTCTCTACCAGGGTGT
  GGGGCATTGGCCAGGAGCCCAACCCTGTTCAGCTGCTGCAGGCATATATAGCCACCAAGACAGAGATGGG
  ACAGCAGATGAGCCCCTCGGAGCTGAGCCACCCTGTGCCCCTGTCCTTCGAGCCTGTCAAGAACTTCTTT
  AAGGAGTTTGTGGAAGCCATCGGGAACACGTTGCAGACACCAATGGACACTCGCCTGAAGGAAAACATAA
  ACTGCCAGCTTTCAAACTCCTCCAGCACCAAGCCAGGCGCCTTGTTGAAAACCAGCCCCTCGCCAGAGAG
  AGCTCTCTTCTTGAAATACGCCCGTACGCCAACGCTGGACCCGGACACGATGCACGCGCGCCTGCGCCTG
  TCGCCGGACGGTCTGACAGTCCGCTGCTCGCTGCTGGGTCGCCTGGGGCCGCGTCCCGCGCCGCGGTTCG
  ACGCGCTGCGGCAGGTGCTGGGCCGCGACGGCTTCGCAGCCGGACGCCACTACTGGGAGGTGGACGTGCA
  GGAGGCCGGTGTGGGCTGGTGGGTGGGCGCGGCGTACCCGTCGCTGCGCCGCCGCGGAGCCTCCGCCGCC
  GCCCGCCTGGGCTGCAACCGCGAGTCGTGGTGCGTAAAGCGTTACGACCTAGAGTACTGGGCCTTCCATG
  ACGGCCAGCGAAGTCGCCTGCGGCCCCGCCGCGACCCCCACCGGCTCGGCGTCTTCCTGGACTACGAGGC
  CGGCATTCTGGCCTTCTACGACGTGGCGGGTGGCATGAGCCACTTGCACACCTTCCACGCCGCCTTCCAG
  GAGCCGCTCTATCCAGCCCTGCGCCTCTGGGAGGGGCCCATCAGCATCCCTAGGTTGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224121 representing NM_029077
  Red=Cloning site Green=Tags(s)
  
  MASETTEARAPFQPDGAYGWRCPEHSERPAELFCRRCGRCVCALCPVLGAHRGHPVGLAEEEAVRVQKLI
  QDCLECLATKKRQHADNIAHLEDAGERLKVYADSSKAWLTQKFTELRLLLDEEEVLAKKFIDKSTQLTLQ
  VYREQAETCGKQIEVMDDFSTRVWGIGQEPNPVQLLQAYIATKTEMGQQMSPSELSHPVPLSFEPVKNFF
  KEFVEAIGNTLQTPMDTRLKENINCQLSNSSSTKPGALLKTSPSPERALFLKYARTPTLDPDTMHARLRL
  SPDGLTVRCSLLGRLGPRPAPRFDALRQVLGRDGFAAGRHYWEVDVQEAGVGWWVGAAYPSLRRRGASAA
  ARLGCNRESWCVKRYDLEYWAFHDGQRSRLRPRRDPHRLGVFLDYEAGILAFYDVAGGMSHLHTFHAAFQ
  EPLYPALRLWEGPISIPRLP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_029077
• ORF Size: 1320 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029077.4, NP_083353.1
• RefSeq Size: 3204 bp
• RefSeq ORF: 1323 bp
• Locus ID: 74735
• UniProt ID: Q8BVW3
• Cytogenetics: 4 B1
• MW: 50.1 kDa
• Gene Summary: Plays a role in the innate immune defense against viruses. Facilitates the type I IFN response by interacting with MAVS at the outer mitochondria membrane and thereby recruiting NF-kappa-B essential modulator IKBKG/NEMO to the MAVS signalosome, leading to the activation of both the IFN regulatory factor 3/IRF3 and NF-kappa-B pathways. Positively regulates the CGAS-induced type I interferon signaling pathway by stabilizing CGAS and inhibiting its autophagic degradation (PubMed:27666593). Inhibits the transcriptional activity of SPI1 in a dose-dependent manner (PubMed:14592421).[UniProtKB/Swiss-Prot Function]