Trim14 (NM_029077) Mouse Untagged Clone

CAT#: MC215846

Trim14 (untagged) - Mouse tripartite motif-containing 14 (Trim14), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trim14
• Synonyms: 5830400N10Rik; pub
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC215846 representing NM_029077
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAGCGAGACTACCGAGGCGCGGGCGCCCTTTCAGCCCGACGGGGCGTATGGCTGGCGCTGTCCGG
  AGCACAGCGAACGCCCGGCTGAGCTCTTCTGTCGCCGCTGCGGCCGCTGTGTGTGCGCGCTCTGCCCGGT
  TCTGGGTGCGCACCGCGGCCACCCGGTGGGCCTGGCCGAGGAGGAGGCTGTGCGTGTGCAGAAGCTAATC
  CAGGACTGTTTAGAGTGTTTGGCAACCAAGAAGAGGCAACATGCGGACAACATAGCCCATTTGGAAGACG
  CCGGGGAAAGGCTCAAGGTTTACGCAGACTCAAGTAAAGCCTGGCTGACACAGAAATTCACAGAACTCAG
  ATTACTCCTGGATGAAGAGGAAGTACTGGCCAAAAAATTCATTGACAAAAGCACACAGCTCACCCTCCAG
  GTGTACCGGGAGCAAGCCGAGACTTGTGGGAAGCAGATTGAGGTCATGGATGACTTCTCTACCAGGGTGT
  GGGGCATTGGCCAGGAGCCCAACCCTGTTCAGCTGCTGCAGGCATATATAGCCACCAAGACAGAGATGGG
  ACAGCAGATGAGCCCCTCGGAGCTGAGCCACCCTGTGCCCCTGTCCTTCGAGCCTGTCAAGAACTTCTTT
  AAGGAGTTTGTGGAAGCCATCGGGAACACGTTGCAGACACCAATGGACACTCGCCTGAAGGAAAACATAA
  ACTGCCAGCTTTCAAACTCCTCCAGCACCAAGCCAGGCGCCTTGTTGAAAACCAGCCCCTCGCCAGAGAG
  AGCTCTCTTCTTGAAATACGCCCGTACGCCAACGCTGGACCCGGACACGATGCACGCGCGCCTGCGCCTG
  TCGCCGGACGGTCTGACAGTCCGCTGCTCGCTGCTGGGTCGCCTGGGGCCGCGTCCCGCGCCGCGGTTCG
  ACGCGCTGCGGCAGGTGCTGGGCCGCGACGGCTTCGCAGCCGGACGCCACTACTGGGAGGTGGACGTGCA
  GGAGGCCGGTGTGGGCTGGTGGGTGGGCGCGGCGTACCCGTCGCTGCGCCGCCGCGGAGCCTCCGCCGCC
  GCCCGCCTGGGCTGCAACCGCGAGTCGTGGTGCGTAAAGCGTTACGACCTAGAGTACTGGGCCTTCCATG
  ACGGCCAGCGAAGTCGCCTGCGGCCCCGCCGCGACCCCCACCGGCTCGGCGTCTTCCTGGACTACGAGGC
  CGGCATTCTGGCCTTCTACGACGTGGCGGGTGGCATGAGCCACTTGCACACCTTCCACGCCGCCTTCCAG
  GAGCCGCTCTATCCAGCCCTGCGCCTCTGGGAGGGGCCCATCAGCATCCCTAGGTTGCCCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_029077
• Insert Size: 1323 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029077.4, NP_083353.1
• RefSeq Size: 3204 bp
• RefSeq ORF: 1323 bp
• Locus ID: 74735
• UniProt ID: Q8BVW3
• Cytogenetics: 4 B1
• Gene Summary: Plays a role in the innate immune defense against viruses. Facilitates the type I IFN response by interacting with MAVS at the outer mitochondria membrane and thereby recruiting NF-kappa-B essential modulator IKBKG/NEMO to the MAVS signalosome, leading to the activation of both the IFN regulatory factor 3/IRF3 and NF-kappa-B pathways. Positively regulates the CGAS-induced type I interferon signaling pathway by stabilizing CGAS and inhibiting its autophagic degradation (PubMed:27666593). Inhibits the transcriptional activity of SPI1 in a dose-dependent manner (PubMed:14592421).[UniProtKB/Swiss-Prot Function]