Ibtk (NM_001081282) Mouse Tagged ORF Clone

CAT#: MR224109

Ibtk (Myc-DDK-tagged) - Mouse inhibitor of Bruton agammaglobulinemia tyrosine kinase (Ibtk)

Product Images

Circular map for MR224109

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ibtk
• Synonyms: 5430411K16Rik; AA409502; BTKI; mKIAA1417
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224109 representing NM_001081282
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGCTGCCACCCCCGACTGCACGTCAAAATGCAGATCCCTGAAGCATGCGCTGGATGTCCTTTCTG
  TGGTGACAAAGGGGAGTGAGAGCCAGATCAAGTCCTTCCTCGCCAGGTACTGCTACAATGCTGCAACTGT
  CAAGGATGCCTTTGGCAGGAACGCCGGCCACCTGGCTTCCTCCTGCGGGAAGAAAGGAGTGTTGGACTGG
  CTGATTGAGAAAGGAGTGGACTTGCTGGTGAAAGACAAGGAGTCCGGCTGGACAGCACTGCACAGAAGCG
  TTTTTTATGGACATATCGATTGTGTTTGGTCTCTGTTGAAGCATGGTGTTAGCTTGTATATGCAAGATAA
  AGAAGGATTGTCACCTTTGGATCTTCTTATGAAGGACAGGCCAACTCATGTTGTATTTAAGGACACTGAT
  CCCACAGAAGTCTATACTTGGGGAGATAACACAAACTTCACTCTGGGCCACGGAAGCCAGAATAGCAAAC
  ATCACCCCGAGCTGCTGGACCTGTTCTCCAGGAGTGGGGTTTATGTCAAGCAGGTGGTGCTTTGCAAATT
  TCATTCGGTGTTTCTGTCCCAGAAAGGGCAGGTTTACACCTGTGGCCATGGTCGCGGAGGACGGTTAGGC
  CATGGAGATGAACAGACATGCTTGGTTCCCAGGCTTGTGGAAGGACTGTCTGGTCACAATTGTTCGCAAG
  TGGCAGCTGCTAAGGATCACACTGTTGTGTTAACTGATGATGGATGTGTCTATACATTTGGTCTCAACAT
  GTTTCACCAATTAGGAATTATTCCTCCACCTGCTAGTTGTAATGTACCCAGACAGATACAGGCAAAATAT
  CTGAAAGGAAGGACTATCATTGGAGTAGCAGCGGGCAGGTTTCATACGGTGCTGTGGACTCGAGAAGCCG
  TTTACACACTGGGACTGAATGGTGGACAGTTGGGTCACTTACTGGATCCCAATGGTGAAAAGTGTGTCAC
  AACCCCTCGCCAAGTCTCTGCCCTTCATCACAAGGATATCGCTGTGTCTTTGGTAGCTGCAAGTGATGGT
  GCCACAGTCTGTGTCACCACAAGGGGAGACATTTACTTACTTGCAGACTATCAGTGCAAGAAGATGGCAA
  CTAAACAACTGAACTTGAAAAAGGTTCTTGTCTCTGGAGGTTGTATGGAATACAAGGTTGATCCTGAACA
  TTTGACAGAAAATGGAGGTCAGAAAATATGTGTCCTCGCTATGGATGGAGCTGGAAGGGTGTTTTGCTGG
  AGATCAATCAGCAGTTCTCTGAAGCAGTGCCGATGGGCCTATCCTCGCCAGGTGTCAATCTCCGATATTG
  CTTTAAATAGAAATGAAATTCTGTTTGTCACACAGGATGGGGAAGGATTTAAAGGGAAATGGTTTGAAGA
  CAAAAGAAAGAATTCTGAAAAGAAAGCAGACATTTTACCAAACCTCCATCATTCCTCATCAGATGTGTCT
  TGTGTCCCTGATACAAATAGTGTGTATGAAAGAATTCGACTTGAGAAACTTCCTTTTGCCCATAGAGCTG
  TTAGTGTCAGCACAGATCCAAGTGGGTGCAACTTTGCAATCCTACAGTCAGACCCTAAAACAAGCCTTTA
  TGAGATTCCAGTTGTGTCTTCATCATCTTTTTTTGAAGAATTTGGCAAGCTCCTGAGGGAGACAGATGAA
  ATGGACAGCTTCCACGATGTGACCTTTCAAGTTGGCAATAGACACTTCCCCGCACATAAATACATTTTGG
  CCGTGCGCTCTGACTTCTTCCAGAAGTTGTTTCTTTCAGATGGCTCTTCTTTAGAGCTGACGGATGTTTA
  CCAGAAAGACGAAGATGCTGCTGGCTGCCATCTCTTTGTGGTGGAGAAAGTCCATCCTGACTTGTTCGAA
  TACCTCTTACAGTTCATGTACACAGATACCTGTGACTTGTTAACTCATGGCTTCAAACCAAGAATGATCG
  TGAAGAGAAAGGCAGAAGACTGTGAGGGCTCCCCAGATTCTCATCTGCACACTGTGAATTGCCATGTGGA
  TGATAAACAGAAGTCAGCATTTGAGGTGTACAGAAGCAACCAGGCCCACACACTTAGTGAACGGCAGAAG
  AGCAAACCCAAATCTTCTAAGAAGGGAAAAGGTGTTGGGGACGACGACCCTGTAAGGATGTTGCAAAGTG
  TTGCTAAGAAGTTTGGCCTCAGTAATTTGAGTAGCAGGTTAGAAGGAGTCAGATTGGAAAATGAAAAAAT
  TAATGTTATTGCGAAGAAAACTGGCAATAAACTGAAGCTAAGTCAGAAAAAATGTTCGTTTCTGTATGAT
  GTCACCATGAAGTCTGTGGATGGAAAGGAGTTCTCCTGCCATAAGTGTGTGCTCTGTGCGAGACTTGAGT
  ATTTCCACAGCATGCTTAGCAGATCGTGGATTGAGGCCTCCAGCTGTGCAGCTCTGGAAATGCCAATCCA
  GTCTGAGATTCTGAAAGTGATTTTGGACTACCTCTACACTGATGAGGCTGTGGTGATAAAAGAGTCTCAA
  AACGTGGACTTTGTTTGTAGTGTTCTTGTCGTGGCTGATCAGCTTCTCATAACTCGGTTAAAGGAGATTT
  GTGAAGTAGCATTGACTGAAAACCTTACCCTTAAGAATGCTGCGATGCTGCTGGAGTTTGCAGCCTTGTA
  CAATGCTGGGCAGTTGAAGCTCTCTTGTTTACAGTTCATAGGACTGAATATGGCAGCTTTACTTGAAGCA
  AGGTCTCTGGATGTTTTAAGTGAGGATGTTTTGAAGGACCTTTCCATATTCTACAGAAAAATGATTCCAG
  CAATGGAGAGAAGAGTCATTACACCGTATCAAGATGGGCCAGATATTAGCTCTATGCAAGTAGAAGACGG
  AGAGGTCTTTTTTAAAGAAGAAATAAACATGGAACCAAATTACTCGGAAACTATGTTCAAGAAGGCGAAA
  ACAAGAGCTAAAAAGAAGCCACGGAAGCGCTCAGACAGTTCAGGAGGCTACACCCTTTCAGATGTTATCC
  AGAGTCCACCGTCTGCAGGATTATTAAAGTCTGCAAAGACCAATTCTGTGGAGTCTCTTCCAGAATTGTT
  GACCTCAGATTCGGAAGGAAGCTATGCAGGAGTGGCGAGTCCCAGAGACCTCCAGTCGCCCGACTTCACA
  GCAGGATTCCATTCGGATAAGGTCGAGGGGAAAGCTAAGCCATACGTGAATGGTATACCTCCTCCATGTA
  CTAGGGAAGATGTAAAGCCGTGGGAGAAATCACCAACAACTAAGTCTGCTCCCCAGTTCATCCCCAGTAA
  CAGAGTGGACACTGCAGCCTCTTCCAGTTGGCTTGCCGGCTCTTGCAGTCCTGTGAGTCCTCCTGTTGTG
  GATCTTAGAACCATCATGGAAACAGAAGAAAACAGACAGAAATATGGCGCAGCACCAAAGTCAAACTTAG
  GAAAGATTATTTCTCACGGAATCAAACTTTCTCAGAAGCAACGGAAAATGATTGCATTGACCACCAAAGA
  AAACAATTCAGGAACGAACAGCATGGAAGCCATTTTGACAGCTCCTTCCAAGTCCCCCAAGCCTGCGAAT
  GCATGGGCACCTCTGCACTCACCTTTATCCAGGTCATTCCGGGATTTTTTACTAGAAGAAAAAAAACCCG
  TTCCTGGCTATGGTTCAGGAGATCATGTCAAAAAAGTTTGTTTTAAAGGAACTGAAAACTCTCCAGCCCT
  AAATGTTGCAAGATGTTCAACCCATGGAACTCCAGGATTAGAAAGCAATCATGTTTCTGATTTTCCACTT
  CTAGACAGTCCCAACCCCTGGCAGTCTTCCTCCCTGGCGGCTTCTCCCGCAGTTGCTCCAGTCACATTTG
  CATCCATTGTAGAAGAAGAGCGGCAACAAGAAGCTGCCCTCATCAGAAGCCGGGAGAAACCCTTAGCTCT
  CATTCAGGTCGAGGAGCATGCAATACAAGACTTGCTGGTTTTCTATGAGGCATTTGGCAACCCCGAGGAG
  TTTGTCGTCGTTGAAAGGGCACCACAGGGGCCTCTGGCAGTGCCTATGTGGAACAAGCATGGCTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224109 representing NM_001081282
  Red=Cloning site Green=Tags(s)
  
  MDAATPDCTSKCRSLKHALDVLSVVTKGSESQIKSFLARYCYNAATVKDAFGRNAGHLASSCGKKGVLDW
  LIEKGVDLLVKDKESGWTALHRSVFYGHIDCVWSLLKHGVSLYMQDKEGLSPLDLLMKDRPTHVVFKDTD
  PTEVYTWGDNTNFTLGHGSQNSKHHPELLDLFSRSGVYVKQVVLCKFHSVFLSQKGQVYTCGHGRGGRLG
  HGDEQTCLVPRLVEGLSGHNCSQVAAAKDHTVVLTDDGCVYTFGLNMFHQLGIIPPPASCNVPRQIQAKY
  LKGRTIIGVAAGRFHTVLWTREAVYTLGLNGGQLGHLLDPNGEKCVTTPRQVSALHHKDIAVSLVAASDG
  ATVCVTTRGDIYLLADYQCKKMATKQLNLKKVLVSGGCMEYKVDPEHLTENGGQKICVLAMDGAGRVFCW
  RSISSSLKQCRWAYPRQVSISDIALNRNEILFVTQDGEGFKGKWFEDKRKNSEKKADILPNLHHSSSDVS
  CVPDTNSVYERIRLEKLPFAHRAVSVSTDPSGCNFAILQSDPKTSLYEIPVVSSSSFFEEFGKLLRETDE
  MDSFHDVTFQVGNRHFPAHKYILAVRSDFFQKLFLSDGSSLELTDVYQKDEDAAGCHLFVVEKVHPDLFE
  YLLQFMYTDTCDLLTHGFKPRMIVKRKAEDCEGSPDSHLHTVNCHVDDKQKSAFEVYRSNQAHTLSERQK
  SKPKSSKKGKGVGDDDPVRMLQSVAKKFGLSNLSSRLEGVRLENEKINVIAKKTGNKLKLSQKKCSFLYD
  VTMKSVDGKEFSCHKCVLCARLEYFHSMLSRSWIEASSCAALEMPIQSEILKVILDYLYTDEAVVIKESQ
  NVDFVCSVLVVADQLLITRLKEICEVALTENLTLKNAAMLLEFAALYNAGQLKLSCLQFIGLNMAALLEA
  RSLDVLSEDVLKDLSIFYRKMIPAMERRVITPYQDGPDISSMQVEDGEVFFKEEINMEPNYSETMFKKAK
  TRAKKKPRKRSDSSGGYTLSDVIQSPPSAGLLKSAKTNSVESLPELLTSDSEGSYAGVASPRDLQSPDFT
  AGFHSDKVEGKAKPYVNGIPPPCTREDVKPWEKSPTTKSAPQFIPSNRVDTAASSSWLAGSCSPVSPPVV
  DLRTIMETEENRQKYGAAPKSNLGKIISHGIKLSQKQRKMIALTTKENNSGTNSMEAILTAPSKSPKPAN
  AWAPLHSPLSRSFRDFLLEEKKPVPGYGSGDHVKKVCFKGTENSPALNVARCSTHGTPGLESNHVSDFPL
  LDSPNPWQSSSLAASPAVAPVTFASIVEEERQQEAALIRSREKPLALIQVEEHAIQDLLVFYEAFGNPEE
  FVVVERAPQGPLAVPMWNKHGC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001081282
• ORF Size: 4056 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081282.1, NM_001081282.2, NP_001074751.1
• RefSeq Size: 5679 bp
• RefSeq ORF: 4059 bp
• Locus ID: 108837
• UniProt ID: Q6ZPR6
• Cytogenetics: 9 E3.1
• MW: 150 kDa
• Gene Summary: Acts as an inhibitor of BTK tyrosine kinase activity, thereby playing a role in B-cell development. Down-regulates BTK kinase activity, leading to interference with BTK-mediated calcium mobilization and NF-kappa-B-driven transcription (By similarity).[UniProtKB/Swiss-Prot Function]