Rbm4 (NM_009032) Mouse Tagged ORF Clone

CAT#: MR223309

Rbm4 (Myc-DDK-tagged) - Mouse RNA binding motif protein 4 (Rbm4)

Product Images

Circular map for MR223309

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rbm4
• Synonyms: 4921506I22Rik; lark; Lark1; Mlark; Rbm4a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223309 representing NM_009032
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGAAGCTGTTCATTGGAAATCTGCCCCGGGAGGCCACAGAGCAGGAGATCCGCTCACTCTTCGAGC
  AGTACGGGAAGGTGCTGGAATGTGACATCATTAAGAATTATGGCTTTGTGCACATAGAGGACAAGACGGC
  CGCTGAGGATGCCATACGCAACCTGCACCACTACAAGCTGCACGGAGTGAACATCAATGTGGAAGCCAGC
  AAGAATAAGAGCAAAGCTTCAACCAAGTTACACGTGGGCAACATCAGCCCCACTTGTACCAACCAAGAGC
  TTCGGGCCAAGTTTGAGGAGTACGGCCCAGTCATCGAATGTGACATCGTGAAAGATTATGCCTTTGTACA
  CATGGAGCGGGCAGAGGATGCGGTGGAGGCCATCAGGGGCCTCGACAACACAGAGTTTCAAGGCAAACGA
  ATGCACGTGCAGTTGTCCACCAGCCGGCTTAGGACCGCGCCCGGGATGGGAGACCAGAGCGGCTGCTATC
  GGTGCGGGAAAGAGGGGCACTGGTCCAAAGAGTGTCCAATAGATCGTTCGGGCCGCGTGGCAGACTTGAC
  CGAGCAATATAATGAGCAATATGGAGCAGTGCGTACGCCTTACACCATGAGTTATGGGGATTCATTGTAT
  TACAACAACACGTACGGAGCGCTCGATGCCTACTACAAGCGCTGCCGTGCTGCCCGGTCCTATGAGGCAG
  TGGCAGCTGCAGCTGCCTCTGCGTATAGTAACTATGCAGAGCAGACTTTGTCTCAGCTGCCACAAGTCCA
  GAACACAGCCATGGCCAGTCACCTCACCTCCACCTCTCTCGATCCCTACAATAGACATCTGTTGCCGCCT
  TCAGGAGCTGCAGCAGCAGCAGCAGCTGCTGCTGCTTGTACTGCAGCTTCTACTTCATATTACGGGCGGG
  ATCGGAGCCCCCTGCGTCGCGCTACAGGCCCAGTCCTCACTGTTGGAGAGGGCTACGGTTACGGGCATGA
  CAGTGAGTTGTCCCAAGCTTCAGCAGCTGCTCGGAATTCCCTGTACGACATGGCCCGGTATGAGCGGGAG
  CAGTATGCGGATCGGGCGCGGTACTCAGCCTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223309 representing NM_009032
  Red=Cloning site Green=Tags(s)
  
  MVKLFIGNLPREATEQEIRSLFEQYGKVLECDIIKNYGFVHIEDKTAAEDAIRNLHHYKLHGVNINVEAS
  KNKSKASTKLHVGNISPTCTNQELRAKFEEYGPVIECDIVKDYAFVHMERAEDAVEAIRGLDNTEFQGKR
  MHVQLSTSRLRTAPGMGDQSGCYRCGKEGHWSKECPIDRSGRVADLTEQYNEQYGAVRTPYTMSYGDSLY
  YNNTYGALDAYYKRCRAARSYEAVAAAAASAYSNYAEQTLSQLPQVQNTAMASHLTSTSLDPYNRHLLPP
  SGAAAAAAAAAACTAASTSYYGRDRSPLRRATGPVLTVGEGYGYGHDSELSQASAAARNSLYDMARYERE
  QYADRARYSAF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009032
• ORF Size: 1083 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009032.3, NP_033058.2
• RefSeq Size: 2630 bp
• RefSeq ORF: 1086 bp
• Locus ID: 19653
• UniProt ID: Q8C7Q4
• Cytogenetics: 19 A
• MW: 40 kDa
• Gene Summary: RNA-binding factor involved in multiple aspects of cellular processes like alternative splicing of pre-mRNA and translation regulation. Modulates alternative 5'-splice site and exon selection. Acts as a muscle cell differentiation-promoting factor. Activates exon skipping of the PTB pre-mRNA during muscle cell differentiation. Antagonizes the activity of the splicing factor PTBP1 to modulate muscle cell-specific exon selection of alpha tropomyosin. Binds to intronic pyrimidine-rich sequence of the TPM1 and MAPT pre-mRNAs. Required for the translational activation of PER1 mRNA in response to circadian clock. Binds directly to the 3' UTR of the PER1 mRNA. Exerts a suppressive activity on Cap-dependent translation via binding to CU-rich responsive elements within the 3' UTR of mRNAs, a process increased under stress conditions or during myocytes differentiation. Recruits EIF4A1 to stimulate IRES-dependent translation initiation in respons to cellular stress. Associates to internal ribosome entry segment (IRES) in target mRNA species under stress conditions. Plays a role for miRNA-guided RNA cleavage and translation suppression by promoting association of AGO2-containing miRNPs with their cognate target mRNAs. Associates with miRNAs during muscle cell differentiation. Binds preferentially to 5'-CGCGCG[GCA]-3' motif in vitro.[UniProtKB/Swiss-Prot Function]