Rbm4 (NM_009032) Mouse Untagged Clone

CAT#: MC209299

Rbm4 (untagged) - Mouse RNA binding motif protein 4 (Rbm4), (10ug)


  "NM_009032" in other vectors (2)

Reconstitution Protocol

USD 503.00

5 Weeks*

Size
    • 10 ug

Product Images

Frequently bought together (3)
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Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Symbol Rbm4
Synonyms 4921506I22Rik; lark; Lark1; Mlark; Rbm4a
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC209299 representing NM_009032
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGAAGCTGTTCATTGGAAATCTGCCCCGGGAGGCCACAGAGCAGGAGATCCGCTCACTCTTCGAGC
AGTACGGGAAGGTGCTGGAATGTGACATCATTAAGAATTATGGCTTTGTGCACATAGAGGACAAGACGGC
CGCTGAGGATGCCATACGCAACCTGCACCACTACAAGCTGCACGGAGTGAACATCAATGTGGAAGCCAGC
AAGAATAAGAGCAAAGCTTCAACCAAGTTACACGTGGGCAACATCAGCCCCACTTGTACCAACCAAGAGC
TTCGGGCCAAGTTTGAGGAGTACGGCCCAGTCATCGAATGTGACATCGTGAAAGATTATGCCTTTGTACA
CATGGAGCGGGCAGAGGATGCGGTGGAGGCCATCAGGGGCCTCGACAACACAGAGTTTCAAGGCAAACGA
ATGCACGTGCAGTTGTCCACCAGCCGGCTTAGGACCGCGCCCGGGATGGGAGACCAGAGCGGCTGCTATC
GGTGCGGGAAAGAGGGGCACTGGTCCAAAGAGTGTCCAATAGATCGTTCGGGCCGCGTGGCAGACTTGAC
CGAGCAATATAATGAGCAATATGGAGCAGTGCGTACGCCTTACACCATGAGTTATGGGGATTCATTGTAT
TACAACAACACGTACGGAGCGCTCGATGCCTACTACAAGCGCTGCCGTGCTGCCCGGTCCTATGAGGCAG
TGGCAGCTGCAGCTGCCTCTGCGTATAGTAACTATGCAGAGCAGACTTTGTCTCAGCTGCCACAAGTCCA
GAACACAGCCATGGCCAGTCACCTCACCTCCACCTCTCTCGATCCCTACAATAGACATCTGTTGCCGCCT
TCAGGAGCTGCAGCAGCAGCAGCAGCTGCTGCTGCTTGTACTGCAGCTTCTACTTCATATTACGGGCGGG
ATCGGAGCCCCCTGCGTCGCGCTACAGGCCCAGTCCTCACTGTTGGAGAGGGCTACGGTTACGGGCATGA
CAGTGAGTTGTCCCAAGCTTCAGCAGCTGCTCGGAATTCCCTGTACGACATGGCCCGGTATGAGCGGGAG
CAGTATGCGGATCGGGCGCGGTACTCAGCCTTTTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_009032
Insert Size 1086 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_009032.3, NP_033058.2
RefSeq Size 2630 bp
RefSeq ORF 1086 bp
Locus ID 19653
UniProt ID Q8C7Q4
Cytogenetics 19 A
Gene Summary RNA-binding factor involved in multiple aspects of cellular processes like alternative splicing of pre-mRNA and translation regulation. Modulates alternative 5'-splice site and exon selection. Acts as a muscle cell differentiation-promoting factor. Activates exon skipping of the PTB pre-mRNA during muscle cell differentiation. Antagonizes the activity of the splicing factor PTBP1 to modulate muscle cell-specific exon selection of alpha tropomyosin. Binds to intronic pyrimidine-rich sequence of the TPM1 and MAPT pre-mRNAs. Required for the translational activation of PER1 mRNA in response to circadian clock. Binds directly to the 3' UTR of the PER1 mRNA. Exerts a suppressive activity on Cap-dependent translation via binding to CU-rich responsive elements within the 3' UTR of mRNAs, a process increased under stress conditions or during myocytes differentiation. Recruits EIF4A1 to stimulate IRES-dependent translation initiation in respons to cellular stress. Associates to internal ribosome entry segment (IRES) in target mRNA species under stress conditions. Plays a role for miRNA-guided RNA cleavage and translation suppression by promoting association of AGO2-containing miRNPs with their cognate target mRNAs. Associates with miRNAs during muscle cell differentiation. Binds preferentially to 5'-CGCGCG[GCA]-3' motif in vitro.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Variants 1, 2 and 3 all encode isoform a.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.