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Shank1 (NM_001034115) Mouse Tagged ORF Clone
CAT#: MR223290
- TrueORF®
Shank1 (Myc-DDK-tagged) - Mouse SH3/ankyrin domain gene 1 (Shank1)
ORF Plasmid: tGFP
"NM_001034115" in other vectors (2)
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Frequently bought together (4)
DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
USD 198.00
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Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Symbol | Shank1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR223290 representing NM_001034115
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCCACAGCCCCGCGACAAGCGAGGACGAGGAAAGACACAGTGCCAGCGAGTGTCCCGAGGGGGGCT CAGAATCGGACAGCTCCCCAGACGGGCCAGGAAGAGGCCCCCAGGGGACTCGGGGCCGAGGCAGCGGAGC ACCTGGTAACCTGGCCTCTACCCGAGGCCTCCAGGGACGCTCCATGTCTGTCCCTGACGACGCCCACTTC AGCATGATGGTTTTCAGGATTGGCATCCCTGACTTGCATCAGACGAAATGCCTACGCTTCAACCCAGATG CCACCATCTGGACAGCCAAACAGCAAGTACTCTGTGCCCTGAGCGAGAGTCTACAGGACGTGCTGAACTA TGGACTGTTCCAGCCAGCCACCTCAGGCCGGGATGCCAACTTCTTGGAGGAGGAGAGGCTTCTAAGAGAG TACCCTCAGTCCTTTGAGAAAGGGGTACCCTACCTGGAGTTCCGCTACAAGACCCGAGTCTACAAACAGA CCAACCTGGATGAGAAACAGCTGGCCAAGCTGCACACAAAGACTGGGTTGAAGAAGTTCCTGGAATATGT GCAGCTTGGGACGTCTGACAAGGTGGCAAGGCTGCTGGACAAGGGGCTGGACCCCAACTACCACGACTCA GATTCAGGAGAGACACCTTTGACCCTGGCGGCCCAGACTGAAGGCTCAGTAGAGGTGATTCGGACGCTGT GCCTGGGCGGGGCGCATATAGACTTCAGGGCCCGGGATGGCATGACAGCGCTTCACAAAGCTGCATGTGC CCGGCACTGCCTGGCGCTCACGGCACTTCTGGACCTTGGGGGCTCCCCCAACTACAAGGACCGACGAGGG CTGACCCCCCTGTTCCACACAGCTATGGTGGGGGGAGACCCCCGCTGCTGCGAGCTACTTCTGTACAACA GGGCCCAGCTGGGCATTGCAGATGAGAACGGCTGGCAGGAAATCCATCAGGCCTGCCAACGAGGGCACTC TCAGCACCTGGAACACCTGCTCTTCTATGGGGCTGAGCCTGGAGCCCAGAATGCCTCAGGAAACACGGCC CTGCACATCTGTGCTCTCTACAACAAGGAGACCTGTGCCAGGATCCTCCTTTATCGTGGAGCAAACAAGG ATGTGAAAAATAACAATGGACAGACACCCTTCCAGGTGGCTGTGATTGCTGGGAATTTTGAGCTGGGGGA ACTGATTCGTAACCATCGGGAACAGGATGTGGTACCTTTCCAGGAGTCCCCCAAGTATGCAGCCCGGCGT CGGGGACCCCCAGGCGCAGGGCTGACTGTACCCCCAGCGCTGCTGCGAGCTAACAGTGACACGAGCATGG CGCTGCCTGATTGGATGGTGTTTTCGGCCCCAGGGGCCTCATCCTCTGGGACCCCTGGCCCTACCTCAGG ATCCCAGGGCCAGTCACAGCCCTCGGCCCCCAGCACCAAACTCAGTAGTGGGACCCTCAGAAGTGCCAGC AGTCCCCGAGGAGCCCGGGCACGCTCCCCGTCCCGGGGGAGACATCCTGAAGACGCCAAGAGACAACCTC GAGGCCGGCCCAGCTCCAGCGGGACACCCCGAGATGGGCCGGCTGGGGGAACTGGGGGCTCAGGGGGCCC TGGGGGCTCCCTGGGCAGCAGAGGCAGACGTAGGAAGCTCTATTCAGCGGTACCCGGTCGCTCCTTCATG GCGGTGAAGTCCTACCAGGCGCAAGGCGAGGGGGAGATCTCTCTGAGTAAAGGCGAAAAGATCAAAGTGC TCAGCATCGGGGAAGGAGGCTTCTGGGAAGGCCAGGTCAAAGGTCGAGTTGGCTGGTTCCCTTCTGACTG CCTAGAAGAGGTGGCAAACCGTTCCCAGGAGGGAAGACAAGAAAGCCGAAGTGACAAGGCAAAGAGACTC TTCAGGCATTACACAGTGGGCTCCTATGACAGCTTCGATGCCCCAAGTCTGATCGATGGGATTGACTCAG GGAGTGATTACATCATCAAGGAGAAGACAGTCTTACTGCAGAAGAAGGACAGTGAGGGGTTCGGGTTCGT GCTCCGGGGGGCCAAGGCGCAGACCCCCATCGAGGAATTCACCCCCACCCCGGCCTTCCCGGCGCTGCAG TACCTCGAGTCTGTGGACGAGGGTGGCGTGGCATGGCGGGCTGGACTACGAATGGGAGACTTCCTCATCG AGGTGAACGGACAGAATGTAGTGAAGGTTGGCCACCGTCAGGTGGTCAACATGATCCGACAGGGAGGCAA CACACTGATGGTCAAGGTGGTGATGGTGACCAGACACCCAGACATGGATGAGGCTGTCCACAAGAAAGCA TCCCAGCAGGCTAAGCGGCTCCCGCCCCCAGCCATCTCCCTGCGTTCCAAGTCTATGACCTCAGAGCTGG AGGAAATGGTCTCCCCCTGGAAGAAGAAAATTGAATACGAACAACAGCCTGCAGCAGTGCCCAGCATGGA GAAAAAGCGGACTGTGTATCAGATGGCACTCAACAAACTGGATGAGATCTTGGCTGCAGCTCAGCAGACC ATCAGTGCAAGTGAGAGTCCTGGGCCTGGCGGCCTCGCATCCCTGGGCAAGCACCGACCCAAAGGATTCT TTGCCACTGAGTCGAGCTTTGATCCTCACCACCGCTCCCAGCCAAGTTATGACCGGCCTTCTTTCCTGCC TCCCGGACCTGGCCTTATGCTCCGGCAGAAATCTATCGGGGCTGCGGAAGATGACAGACCCTACCTAGCA CCCCCAGCCATGAAATTCAGTCGTAGCCTGTCAGTCCCGGGTTCAGAGGACATACCCCCACCACCCACTA CATCCCCACCAGAGCCGCCCTATAGCACACCTCCGGCCCCCTCCTCTTCCGGCCGCCTCACTCCCTCGCC TCGGGGAGGGCCCTTTAACCCTGGTTCCGGGGGTCCCCTTCCCGCCTCCTCACCTTCATCTTTCGATGGG CCATCTCCTCCTGACCCCCGTAGTGGGGGCCGAGAGAAGAGCCTATATCACAGTGGCGCCCTACCCCCTG CCCACCACCACCCACCTCATCACCACCATCATCACGCCCCACCTCCTCAGCCCCACCATCACCATGCGCA TCCTCCCCACCCACCGGAGATGGAGACAGGAGGTTCCCCCGACGATCCCCCACCTCGATTGGCTCTGGGA CCCCAGCCCAGCCTTCGAGGCTGGCGAGGTGGAGGACCCAGCCCGACCTCGGGGGCCCCATCACCTTCCC ACCACAGTAGCAGCGGAGGCAGTAGCGGTCCTGCTCAGGCTCCCGCCCTGCGCTACTTCCAGCTGCCCCC GAGGGCGGCCAGTGCGGCTATGTACGTACCAGCCCGCTCCGGCCGCGGTCGCAAAGGCCCGCTGGTCAAG CAGACCAAAGTGGAAGGCGAGCCCCAAAAGGGCAGCCTCCCTCCCGCGTCCTCTCCAACATCCCCGGCGT TGCCTCGGTCCGAACCACCTCCGGCCGGGCCGTCGGAGAAAAATTCCATCCCTATCCCTACCATCATCAT CAAAGCCCCATCTACCAGTAGCAGCGGCCGCAGTAGCCAGGGAAGCAGCACAGAGGCTGAGCCCCCCACC CAGCCCGACGGCGCGGGCGGCGGAGGATCCTCGCCTAGCCCTGCACCTGCCACGTCCCCTGTACCGCCGT CCCCGTCGCCCGTGCCCACCCCTGCGTCACCCAGCGGCCCGGCCACCCTGGATTTCACTAGCCAGTTTGG AGCAGCCCTAGTGGGCGCGGCGCGGAGGGAGGGAGGCTGGCAAAACGAAGCCCGGCGGCGGTCCACGCTA TTCCTGTCCACCGACGCAGGAGATGAGGATGGAGGCGACAGCGGGCTGGGCCCGGGGGCGCCCCCGGGTC CCCGGCTGCGCCATTCCAAATCCATTGATGAAGGCATGTTCTCCGCCGAGCCGTACCTCCGGCTGGAGTC GGGGGGCAGCAGCGGGGGCTACGGGGCATACGCAGCGGGCAGCCGAGCCTACGGGGGCAGCGGGAGCAGC AGCGCCTTCACCAGCTTCCTCCCCCCGCGGCCCCTGGTGCATCCGCTGACCGGCAAAGCCTTGGATCCCG CCTCTCCGCTGGGGCTGGCCTTGGCCGCCCGGGAGCGGGCCTTGAAGGAGTCCTCGGAGGGCGGGGTCAC CCCTCAGCCGCCCCCGAGACCTCCATCACCACGCTACGACGCCCCACCGCCCACCCTTCACCACCACTCG CCCCATTCACCCCATTCGCCACATGCGCGTCATGAGCCAGTGTTGCGCCTCTGGGGAGACCCGGCGCGCC GGGAGCTGGGGTACCGGGCAGGCCTAGGGAGTCAGGAGAAGGCCCTTACCGCTAGTCCACCTGCAGCTCG CCGCTCGCTACTGCACCGTCTTCCCCCGACGGCTCCCGGGGTCGGGCCGCTCCTGCTTCAACTGGGGCCG GAGCCCCCAACTCCCCATCCTGGGGTGAGCAAGGCCTGGCGCACAGCAGCCCCCGAGGAGCCAGAGCGGC TGCCTCTGCATGTGCGATTCCTTGAAAATTGCCAGGCCCGGCCTCCTCCAGCTGGCACGAGAGGGTCCTC CACTGAAGATGGGCCTGGGGTTCCGCCTCCCAGCCCTCGCAGAGTCTTGCCAACCTCTCCCACTTCCCCG CGAGGCAACGAGGAGAACGGCCTTCCGCTACTGGTCCTGCCACCGCCAGCCCCTTCGGTGGATGTGGACG ACGGCGAGTTCTTGTTTGCGGAGCCGCTGCCTCCACCCCTGGAGTTTTCCAATAGCTTCGAAAAGCCAGA GTCGCCTCTCACACCCGGGCCTCCCCACCCGCTGCCTGACCCTCCCTCCCCAGCTACCCCTCTACCCGCA GCCCCACCTCCGGCTGTGGCCGCCGCCCCTCCCACCTTGGACTCCACAGCATCCAGCCTGACCTCTTATG ACAGCGAAGTGGCCACGTTGACCCAGGGGGCTCCCGCGGCTCCTGGGGACCCTCCTGCTCCAGGCCCTCC GGCCCCGGCAGCTCCAGCTCCTCCAGCACCGCAGCCAGGCCCCGACCCTCCACCTGGCACAGATTCTGGA ATTGAGGAGGTGGACAGTCGGAGCAGCAGCGATCACCCGCTGGAGACCATCAGCAGTGCATCCACACTCA GCAGCTTGTCCGCCGAAGGAGGAGGCAACACAGGGGGCGTGGCTGGGGGCGGGGCCGGGGTGGCCAGCGG GACGGAGCTCCTGGACACCTACGTTGCCTACCTGGATGGCCAGGCCTTCGGGGGCAGTGGGACTCCTGGC CCACCATATCCCCCTCAGCTCATGACTCCTTCTAAGCTCCGGGGCAGGGCGCTTGGGACTAGTGGAAATT TGAGACCTGGTCCCAGTGGGGGACTTCGAGATCCTGTCACCCCCACCAGCCCCACCGTCTCTGTGACAGG AGCTGGAACAGATGGGCTACTGGCCTTGAGTGCTTGTTCTGGACCCTCAACGGCAGGTGTGGCAGGGGGT CCCGTGGCGGTTGAGCCAGAAGTCCCGCCTGTACCTTTGCCCACTGCCTCATCCCTGCCTCGGAAGCTAC TACCATGGGAGGAGGGTCCGGGACCACCACCACCACCCCTGCCAGGGCCCCTGTCCCAGCCTCAGGCCTC TGCCTTGGCCACGGTCAAAGCCAGCATCATCAGCGAACTCAGCTCCAAGCTCCAGCAGTTTGGTGGGGCC TCTACCGCGGGAGGAGCTCTGCCTTGGGCTCGGGGAGGCAGCGGGGGCAGCACAGACAGCCACCACGGAG GAGCCAGTTACATCCCAGAGAGGACCTCCTCCCTGCAGCGGCAGAGGCTCTCCGAAGACTCCCAGACCTC TCTCCTCTCCAAACCCAGCAGCAGCATCTTTCAGAACTGGCCTAAACCCCCTCTGCCACCACTCCCCACC GGATCTGGGGTCTCCTCTTCAACAGCTGCAGCCCCAGGAGCTACATCCCCTTCAGCCTCATCTGCCTCAG CTTCCACCAGACACCTGCAGGGTGTGGAATTTGAGATGCGGCCTCCGCTGCTCCGCCGGGCACCCAGCCC CTCGCTGCTGCCTGCCTCGGATCACAAGGTCAGCCCAGCGCCCAGGCCCTCTTCTTTGCCCATCCTGCCT TCAGGACCCCTCTACCCGGGCCTCTTCGACATCCGTAGCTCTCCGACCGGAGGGGCAGGAGGCTCGGCTG ACCCCTTCGCTCCCGTCTTTGTGCCACCACACCCCGGGATATCTGGGGGTCTTGGAGGAGCCTTGTCTGG GGCCTCTCGCTCCCTATCACCCACCCGCCTGCTCTCGCTGCCCCCGGACAAGCCGTTCGGCGCCAAGCCT CTAGGGTTCTGGACCAAGTTTGACGTCGCTGATTGGCTGGAGTGGCTGGGCTTATCAGAGCATCGAGCCC AGTTCCTGGACCACGAGATTGATGGCTCCCATCTGCCCGCCTTGACCAAGGAGGACTATGTGGACCTGGG CGTGACCAGGGTGGGCCACCGTATGAACATTGACCGGGCTCTCAAATTTTTCCTGGAGAGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR223290 representing NM_001034115
Red=Cloning site Green=Tags(s) MTHSPATSEDEERHSASECPEGGSESDSSPDGPGRGPQGTRGRGSGAPGNLASTRGLQGRSMSVPDDAHF SMMVFRIGIPDLHQTKCLRFNPDATIWTAKQQVLCALSESLQDVLNYGLFQPATSGRDANFLEEERLLRE YPQSFEKGVPYLEFRYKTRVYKQTNLDEKQLAKLHTKTGLKKFLEYVQLGTSDKVARLLDKGLDPNYHDS DSGETPLTLAAQTEGSVEVIRTLCLGGAHIDFRARDGMTALHKAACARHCLALTALLDLGGSPNYKDRRG LTPLFHTAMVGGDPRCCELLLYNRAQLGIADENGWQEIHQACQRGHSQHLEHLLFYGAEPGAQNASGNTA LHICALYNKETCARILLYRGANKDVKNNNGQTPFQVAVIAGNFELGELIRNHREQDVVPFQESPKYAARR RGPPGAGLTVPPALLRANSDTSMALPDWMVFSAPGASSSGTPGPTSGSQGQSQPSAPSTKLSSGTLRSAS SPRGARARSPSRGRHPEDAKRQPRGRPSSSGTPRDGPAGGTGGSGGPGGSLGSRGRRRKLYSAVPGRSFM AVKSYQAQGEGEISLSKGEKIKVLSIGEGGFWEGQVKGRVGWFPSDCLEEVANRSQEGRQESRSDKAKRL FRHYTVGSYDSFDAPSLIDGIDSGSDYIIKEKTVLLQKKDSEGFGFVLRGAKAQTPIEEFTPTPAFPALQ YLESVDEGGVAWRAGLRMGDFLIEVNGQNVVKVGHRQVVNMIRQGGNTLMVKVVMVTRHPDMDEAVHKKA SQQAKRLPPPAISLRSKSMTSELEEMVSPWKKKIEYEQQPAAVPSMEKKRTVYQMALNKLDEILAAAQQT ISASESPGPGGLASLGKHRPKGFFATESSFDPHHRSQPSYDRPSFLPPGPGLMLRQKSIGAAEDDRPYLA PPAMKFSRSLSVPGSEDIPPPPTTSPPEPPYSTPPAPSSSGRLTPSPRGGPFNPGSGGPLPASSPSSFDG PSPPDPRSGGREKSLYHSGALPPAHHHPPHHHHHHAPPPQPHHHHAHPPHPPEMETGGSPDDPPPRLALG PQPSLRGWRGGGPSPTSGAPSPSHHSSSGGSSGPAQAPALRYFQLPPRAASAAMYVPARSGRGRKGPLVK QTKVEGEPQKGSLPPASSPTSPALPRSEPPPAGPSEKNSIPIPTIIIKAPSTSSSGRSSQGSSTEAEPPT QPDGAGGGGSSPSPAPATSPVPPSPSPVPTPASPSGPATLDFTSQFGAALVGAARREGGWQNEARRRSTL FLSTDAGDEDGGDSGLGPGAPPGPRLRHSKSIDEGMFSAEPYLRLESGGSSGGYGAYAAGSRAYGGSGSS SAFTSFLPPRPLVHPLTGKALDPASPLGLALAARERALKESSEGGVTPQPPPRPPSPRYDAPPPTLHHHS PHSPHSPHARHEPVLRLWGDPARRELGYRAGLGSQEKALTASPPAARRSLLHRLPPTAPGVGPLLLQLGP EPPTPHPGVSKAWRTAAPEEPERLPLHVRFLENCQARPPPAGTRGSSTEDGPGVPPPSPRRVLPTSPTSP RGNEENGLPLLVLPPPAPSVDVDDGEFLFAEPLPPPLEFSNSFEKPESPLTPGPPHPLPDPPSPATPLPA APPPAVAAAPPTLDSTASSLTSYDSEVATLTQGAPAAPGDPPAPGPPAPAAPAPPAPQPGPDPPPGTDSG IEEVDSRSSSDHPLETISSASTLSSLSAEGGGNTGGVAGGGAGVASGTELLDTYVAYLDGQAFGGSGTPG PPYPPQLMTPSKLRGRALGTSGNLRPGPSGGLRDPVTPTSPTVSVTGAGTDGLLALSACSGPSTAGVAGG PVAVEPEVPPVPLPTASSLPRKLLPWEEGPGPPPPPLPGPLSQPQASALATVKASIISELSSKLQQFGGA STAGGALPWARGGSGGSTDSHHGGASYIPERTSSLQRQRLSEDSQTSLLSKPSSSIFQNWPKPPLPPLPT GSGVSSSTAAAPGATSPSASSASASTRHLQGVEFEMRPPLLRRAPSPSLLPASDHKVSPAPRPSSLPILP SGPLYPGLFDIRSSPTGGAGGSADPFAPVFVPPHPGISGGLGGALSGASRSLSPTRLLSLPPDKPFGAKP LGFWTKFDVADWLEWLGLSEHRAQFLDHEIDGSHLPALTKEDYVDLGVTRVGHRMNIDRALKFFLER TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001034115 |
| ORF Size | 6501 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001034115.1, NP_001029287.1 |
| RefSeq Size | 7596 bp |
| RefSeq ORF | 6504 bp |
| Locus ID | 243961 |
| UniProt ID | D3YZU1 |
| Cytogenetics | 7 B3 |
| MW | 226.3 kDa |
| Gene Summary | Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors, and the actin-based cytoskeleton. Plays a role in the structural and functional organization of the dendritic spine and synaptic junction. Overexpression promotes maturation of dendritic spines and the enlargement of spine heads via its ability to recruit Homer to postsynaptic sites, and enhances presynaptic function (By similarity).[UniProtKB/Swiss-Prot Function] |
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