Syce1l (NM_001048145) Mouse Tagged ORF Clone

CAT#: MR222444

Syce1l (Myc-DDK-tagged) - Mouse synaptonemal complex central element protein 1 like (Syce1l), transcript variant 1

Product Images

Circular map for MR222444

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Syce1l
• Synonyms: 4930481F22Rik; MMRP; mmrp2; PSESS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222444 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCGGAGCTGGAGCCTTTGATGGCGGAGTGGCTGGGAGCTGAGAAAGCTGAAGACACCCGAGGGC
  AGGCCGCATCTCTGAATACGATGGAAGACTTGCTGGAGACAGTGAAAAAGCTACAGAAAGAGGGAAGCCT
  GGAGCCCCAGATTGAGGACCTGATCCACCGCATTAATGAGCTGCAGCAAGGTCCAGCAAAGAAGAGATCC
  AGTGAGGAGCTGGGAGAGGCTCAAGCACTCCAGGAAGCCATGCACAGGGAACTAGACTCCTTAAATGAAG
  AGAGAGTGCACCTAGAGGAGGTCCTGCGCAAAAAGCAAGAGGCTGTGTCAATTCTCAAGAAGCATCCCCA
  AGAGAGAGACAGTGACACTCCACACTTGGATGCTCAACAACTGGAGGAGAGGCTGGCGGATCTGGCAAGG
  CAACACAAGGACCTCTGGGAGTTCCATGTCCTGCAGCAGCGACTGGCCCAGGAGATCAGCACGATGGAGC
  ACAGGAAAGATCAGCTTCTTGCAGAGAGGACTCTGCTGCGAGCCAGGCTGGAGAAGGTGGAGCAGCGGCT
  GCAGGAGGCCAGAGAGACCTGGGACAGCCCAGGGAACTGTGGGCTGAAGACAGAGCTGGAGGAATTGGAG
  GGGCAGTCCCAGCGCAGCCCAGAAGCTCAGAATGATAAAGGGGAGGCTAGCCAAGAGGAACAGCATCATT
  TGGAGACATCAGAAGAGTTGCCAAGGACAGGAACGCTCTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222444 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAELEPLMAEWLGAEKAEDTRGQAASLNTMEDLLETVKKLQKEGSLEPQIEDLIHRINELQQGPAKKRS
  SEELGEAQALQEAMHRELDSLNEERVHLEEVLRKKQEAVSILKKHPQERDSDTPHLDAQQLEERLADLAR
  QHKDLWEFHVLQQRLAQEISTMEHRKDQLLAERTLLRARLEKVEQRLQEARETWDSPGNCGLKTELEELE
  GQSQRSPEAQNDKGEASQEEQHHLETSEELPRTGTLC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001048145
• ORF Size: 744 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001048145.1, NP_001041610.1
• RefSeq Size: 837 bp
• RefSeq ORF: 744 bp
• Locus ID: 668110
• UniProt ID: Q5D525
• Cytogenetics: 8 E1
• MW: 28.5 kDa
• Gene Summary: May be involved in meiosis. Isoform 1 may be involved in meiosis during spermatogenesis while isoform 2 is probably related to a later stage of meiosis, in the development stage of secondary spermatocytes and spermatids.[UniProtKB/Swiss-Prot Function]