Mfrp (NM_001190314) Mouse Tagged ORF Clone

CAT#: MR221748

Mfrp (Myc-DDK-tagged) - Mouse membrane-type frizzled-related protein (Mfrp), transcript variant 2

Product Images

Circular map for MR221748

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mfrp
• Synonyms: rd6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR221748 representing NM_001190314
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGACTATGACGACGTCATCCTCCGTCCAGAGGCGAGTGAGTTGAGCAAGACAGAGTTCTGTAATC
  CTGCTTTCGACCCGGAAGCAGGGCCATCCTGCCCTCCACCAGCGTTACAGAGGGATGTCGGCAGCCGGCT
  CCAAGCCCCCTGGCATGCCCAGCGTCTCCGGGGGCTACAGCCCGACTGCCACTTCTCCTGGTTCTGTATT
  CTGCTTCTCAGTGGCCTGCTGCTCCTGCTGCTGGGGCTACTGGTGGCTGTCATCCTGGCTCAGTTGCAGG
  CTACATCCCTCCCCAGGACTACCAAGAACCCACTGCTCACCCGAGGCCTCACCCCCATGGGTGTCATTCC
  CAGCACCACCCCTAACACCACCACCACCACCACCACTACCACCCCAGCAAGGACAGGGCAGCAGGAGGCA
  GCCATGAGCCCTACACACCAGACCACCTGTGGAGGCCTCCTTCCTGGTCCCAGTGGTTTCTTCAGCAGCC
  CTAACTACCCAGACCTCTACCCACCCCTCAGCCACTGTGTCTGGCATATCCAGGTAGCCGCGGGCCAGAC
  AATACAGCTCAAGATTCAAGCCCTCAGCATAGAGAGTATGCTCACCTGTCTTTTTGATCGCTTGGAAATT
  ATCTCAGAGCCTACAGGCCCTCTCCTCAGGGTGTGTGGTAAAACACCTCCTGCCACATTAAACACCAATA
  CCAGCCACCTCCGTGTGTCCTTCGTCTCTGATAACGATGTGGAAGGGTCTGGTTTCCAGGCCTGGTACCA
  AGCAGTGGCCCCTGGACATTGGAGCTGTGCCCATAATGAGTTCCACTGTGACCTTCTCCTCTGCCTGAAG
  CGTGACTCTGTATGTGACGGTATTACCGAGTGTGCCGATGGCAGTGACGAGGCCAACTGCAGTGCCAAGA
  CGTTGGGGTGTGGAGGGAACCTGACTGGGCTCTATGGCGTATTCTCTACCCCAAACTATCCACAGCACTA
  CCCTCACCAACAGCTTTGCACCTGGTACATCGAAGTGCCTGTGGGATACGGGATAAGACTGGAGTTCCAC
  AACTTCAGTCTGGAAGCACAGGCTGAGTGCAAGTTTGACTACGTGGAAGTGTACGAGGCCAGCAACCTGG
  GAACCTTCAGCTTCCTGGGCAGGTTCTGTGGAGCAGAGCCACCACTCAACGTTGTCTCCTCGATGCACCA
  GCTGGCTGTAATCTTCAAGACGGATCTTGGTATCAGCAGCGGGGGCTTTCTAGCCACCTACCAGGCCATC
  AATACTACAGAGAAGTTCTGCCAGAGCGGAGGATATAGGGATCTGCAATGGATGTGTGACTTATGGAAAG
  ACTGTGCAAATGACAGCAACGACAACTGCAGCAGCCACTTGTCCCCACAACCAGACCTGACCTGTGAACC
  TGTCCAGGTGGAGATGTGCCTTGGACTAAGCTACAATACCACGGCCTTTCCTAACATCTGGGTGGGCCTG
  GCCACGCAGACAGAGGTGACAGACATCCTCCGAGGCTACAAGAGTCTGACAAGTCTACCCTGCTACCAGA
  CTTTCCAGAGGTTCCTCTGTGGACTGCTTGTGCCTCGATGCACCTCACTGGGCACTATCCTACCCCCTTG
  TCGTTCCGTCTGCCAGGCGGCGGAGCAGCAGTGCCAGTCTAGCCTGGCATTATTGGGCACCCCCTGGCCT
  TTCAACTGCAACAGGCTGCCCGTGGCAGCTAGCCTGGAAGCTTGCTCCCAGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR221748 representing NM_001190314
  Red=Cloning site Green=Tags(s)
  
  MKDYDDVILRPEASELSKTEFCNPAFDPEAGPSCPPPALQRDVGSRLQAPWHAQRLRGLQPDCHFSWFCI
  LLLSGLLLLLLGLLVAVILAQLQATSLPRTTKNPLLTRGLTPMGVIPSTTPNTTTTTTTTTPARTGQQEA
  AMSPTHQTTCGGLLPGPSGFFSSPNYPDLYPPLSHCVWHIQVAAGQTIQLKIQALSIESMLTCLFDRLEI
  ISEPTGPLLRVCGKTPPATLNTNTSHLRVSFVSDNDVEGSGFQAWYQAVAPGHWSCAHNEFHCDLLLCLK
  RDSVCDGITECADGSDEANCSAKTLGCGGNLTGLYGVFSTPNYPQHYPHQQLCTWYIEVPVGYGIRLEFH
  NFSLEAQAECKFDYVEVYEASNLGTFSFLGRFCGAEPPLNVVSSMHQLAVIFKTDLGISSGGFLATYQAI
  NTTEKFCQSGGYRDLQWMCDLWKDCANDSNDNCSSHLSPQPDLTCEPVQVEMCLGLSYNTTAFPNIWVGL
  ATQTEVTDILRGYKSLTSLPCYQTFQRFLCGLLVPRCTSLGTILPPCRSVCQAAEQQCQSSLALLGTPWP
  FNCNRLPVAASLEACSQP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001190314
• ORF Size: 1737 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001190314.1, NP_001177243.1
• RefSeq Size: 4274 bp
• RefSeq ORF: 1737 bp
• Locus ID: 259172
• UniProt ID: Q8K480
• Cytogenetics: 9 24.6 cM
• MW: 63.1 kDa
• Gene Summary: The protein encoded by this gene contains a region with similarity to the cysteine-rich domain (CRD) of frizzled, a gene originally found in Drosophila that controls tissue polarity. This protein functions in eye development, where it is necessary for the maintenance of photoreceptor outer segments. Mutations in this gene cause retinal degeneration 6 in mice, which gives rise to a mouse model for human retinitis punctata albescens. Bicistronic transcripts composed of the coding sequences for this gene (Mfrp) and the C1q and tumor necrosis factor related protein 5 gene (C1qtnf5) have been identified, and the resulting products can interact with each other. Co-transcription of C1qtnf5 and Mfrp has been observed in both human and mouse. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]