Mfrp (NM_001190314) Mouse Untagged Clone

CAT#: MC219233

Mfrp (untagged) - Mouse membrane-type frizzled-related protein (Mfrp), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mfrp
• Synonyms: rd6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219233 representing NM_001190314
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGACTATGACGACGTCATCCTCCGTCCAGAGGCGAGTGAGTTGAGCAAGACAGAGTTCTGTAATC
  CTGCTTTCGACCCGGAAGCAGGGCCATCCTGCCCTCCACCAGCGTTACAGAGGGATGTCGGCAGCCGGCT
  CCAAGCCCCCTGGCATGCCCAGCGTCTCCGGGGGCTACAGCCCGACTGCCACTTCTCCTGGTTCTGTATT
  CTGCTTCTCAGTGGCCTGCTGCTCCTGCTGCTGGGGCTACTGGTGGCTGTCATCCTGGCTCAGTTGCAGG
  CTACATCCCTCCCCAGGACTACCAAGAACCCACTGCTCACCCGAGGCCTCACCCCCATGGGTGTCATTCC
  CAGCACCACCCCTAACACCACCACCACCACCACCACTACCACCCCAGCAAGGACAGGGCAGCAGGAGGCA
  GCCATGAGCCCTACACACCAGACCACCTGTGGAGGCCTCCTTCCTGGTCCCAGTGGTTTCTTCAGCAGCC
  CTAACTACCCAGACCTCTACCCACCCCTCAGCCACTGTGTCTGGCATATCCAGGTAGCCGCGGGCCAGAC
  AATACAGCTCAAGATTCAAGCCCTCAGCATAGAGAGTATGCTCACCTGTCTTTTTGATCGCTTGGAAATT
  ATCTCAGAGCCTACAGGCCCTCTCCTCAGGGTGTGTGGTAAAACACCTCCTGCCACATTAAACACCAATA
  CCAGCCACCTCCGTGTGTCCTTCGTCTCTGATAACGATGTGGAAGGGTCTGGTTTCCAGGCCTGGTACCA
  AGCAGTGGCCCCTGGACATTGGAGCTGTGCCCATAATGAGTTCCACTGTGACCTTCTCCTCTGCCTGAAG
  CGTGACTCTGTATGTGACGGTATTACCGAGTGTGCCGATGGCAGTGACGAGGCCAACTGCAGTGCCAAGA
  CGTTGGGGTGTGGAGGGAACCTGACTGGGCTCTATGGCGTATTCTCTACCCCAAACTATCCACAGCACTA
  CCCTCACCAACAGCTTTGCACCTGGTACATCGAAGTGCCTGTGGGATACGGGATAAGACTGGAGTTCCAC
  AACTTCAGTCTGGAAGCACAGGCTGAGTGCAAGTTTGACTACGTGGAAGTGTACGAGGCCAGCAACCTGG
  GAACCTTCAGCTTCCTGGGCAGGTTCTGTGGAGCAGAGCCACCACTCAACGTTGTCTCCTCGATGCACCA
  GCTGGCTGTAATCTTCAAGACGGATCTTGGTATCAGCAGCGGGGGCTTTCTAGCCACCTACCAGGCCATC
  AATACTACAGAGAAGTTCTGCCAGAGCGGAGGATATAGGGATCTGCAATGGATGTGTGACTTATGGAAAG
  ACTGTGCAAATGACAGCAACGACAACTGCAGCAGCCACTTGTCCCCACAACCAGACCTGACCTGTGAACC
  TGTCCAGGTGGAGATGTGCCTTGGACTAAGCTACAATACCACGGCCTTTCCTAACATCTGGGTGGGCCTG
  GCCACGCAGACAGAGGTGACAGACATCCTCCGAGGCTACAAGAGTCTGACAAGTCTACCCTGCTACCAGA
  CTTTCCAGAGGTTCCTCTGTGGACTGCTTGTGCCTCGATGCACCTCACTGGGCACTATCCTACCCCCTTG
  TCGTTCCGTCTGCCAGGCGGCGGAGCAGCAGTGCCAGTCTAGCCTGGCATTATTGGGCACCCCCTGGCCT
  TTCAACTGCAACAGGCTGCCCGTGGCAGCTAGCCTGGAAGCTTGCTCCCAGCCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001190314
• Insert Size: 1737 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001190314.1, NP_001177243.1
• RefSeq Size: 4274 bp
• RefSeq ORF: 1737 bp
• Locus ID: 259172
• UniProt ID: Q8K480
• Cytogenetics: 9 24.6 cM
• Gene Summary: The protein encoded by this gene contains a region with similarity to the cysteine-rich domain (CRD) of frizzled, a gene originally found in Drosophila that controls tissue polarity. This protein functions in eye development, where it is necessary for the maintenance of photoreceptor outer segments. Mutations in this gene cause retinal degeneration 6 in mice, which gives rise to a mouse model for human retinitis punctata albescens. Bicistronic transcripts composed of the coding sequences for this gene (Mfrp) and the C1q and tumor necrosis factor related protein 5 gene (C1qtnf5) have been identified, and the resulting products can interact with each other. Co-transcription of C1qtnf5 and Mfrp has been observed in both human and mouse. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1, resulting in an isoform (2) that is shorter than isoform 1.