Dntt (NM_009345) Mouse Tagged ORF Clone

CAT#: MR221097

  • TrueORF®

Dntt (Myc-DDK-tagged) - Mouse deoxynucleotidyltransferase, terminal (Dntt), transcript variant 1

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro


  "NM_009345" in other vectors (4)


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Reconstitution Protocol

USD 740.00

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Size
    • 10 ug

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Frequently bought together (3)
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DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Dntt
Synonyms Tdt
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR221097 representing NM_009345
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATCCACTGCAAGCAGTCCACCTGGGTCCTCGGAAGAAAAGACCCAGGCAGCTGGGCACCCCAGTGG
CTTCCACGCCTTATGACATCCGATTTCGAGACTTGGTCCTCTTCATTTTGGAAAAGAAGATGGGAACAAC
TCGAAGAGCCTTCCTCATGGAGCTGGCCCGAAGGAAAGGGTTCAGGGTGGAAAATGAGCTCAGTGATTCG
GTCACCCACATTGTGGCAGAGAACAACTCAGGGTCAGACGTCCTGGAGTGGCTCCAGCTGCAGAACATCA
AAGCCAGCTCCGAGCTCGAACTCTTGGACATCTCCTGGCTCATCGAATGCATGGGAGCTGGGAAGCCAGT
GGAGATGATGGGGAGACATCAGCTTGTTGTGAATAGAAACTCCTCCCCGAGTCCTGTCCCAGGCTCCCAG
AATGTTCCAGCACCTGCTGTGAAGAAGATCTCCCAATATGCTTGCCAGCGAAGAACCACATTAAACAATT
ACAACCAACTATTCACGGATGCCCTTGATATCCTGGCTGAAAATGATGAGCTTAGAGAGAATGAAGGTTC
TTGCCTGGCATTCATGCGAGCGTCCTCTGTACTGAAATCTCTGCCATTCCCCATCACCAGCATGAAAGAC
ACAGAGGGGATTCCTTGCCTAGGGGACAAGGTGAAGAGTATCATAGAGGGAATTATTGAAGATGGAGAAA
GTTCTGAAGCTAAAGCTGTGTTAAATGATGAGCGATATAAATCCTTCAAACTCTTTACCTCTGTGTTTGG
TGTGGGACTGAAGACAGCTGAGAAATGGTTCAGGATGGGTTTCAGAACTCTCAGCAAAATACAATCAGAC
AAAAGCCTGAGGTTTACACAAATGCAGAAAGCAGGATTCCTCTACTACGAAGACCTCGTTAGCTGTGTGA
ACAGGCCAGAAGCCGAGGCTGTCAGCATGCTAGTTAAGGAGGCGGTTGTGACATTTCTTCCAGATGCCTT
GGTCACCATGACTGGGGGGTTCCGCAGGGGTAAGATGACTGGACATGATGTAGACTTTCTAATTACCAGC
CCAGAAGCCACAGAGGATGAAGAGCAGCAGCTCTTGCATAAAGTGACAGACTTTTGGAAGCAGCAGGGGT
TGCTTTTGTACTGCGACATCTTAGAGTCAACCTTTGAAAAGTTCAAGCAGCCCAGCAGGAAGGTGGATGC
TCTCGACCATTTCCAGAAATGCTTCCTGATTCTGAAGCTGGACCACGGGAGAGTGCACAGCGAGAAGAGC
GGCCAGCAGGAAGGAAAGGGCTGGAAGGCCATCCGTGTAGATCTGGTCATGTGCCCCTATGATCGCCGGG
CCTTCGCCCTGCTTGGATGGACCGGCTCCAGACAGTTTGAGAGAGACTTGCGGCGCTATGCCACACACGA
GAGGAAGATGATGCTGGACAACCACGCCCTGTATGACAGGACTAAGGGGAAGACTGTCACGATTTCACCT
TTGGATGGGAAAGTGTCAAAACTACAGAAAGCCTTGAGGGTGTTTCTTGAAGCAGAAAGTGAAGAAGAGA
TCTTTGCCCATCTGGGACTGGACTACATTGAACCATGGGAAAGAAATGCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR221097 representing NM_009345
Red=Cloning site Green=Tags(s)

MDPLQAVHLGPRKKRPRQLGTPVASTPYDIRFRDLVLFILEKKMGTTRRAFLMELARRKGFRVENELSDS
VTHIVAENNSGSDVLEWLQLQNIKASSELELLDISWLIECMGAGKPVEMMGRHQLVVNRNSSPSPVPGSQ
NVPAPAVKKISQYACQRRTTLNNYNQLFTDALDILAENDELRENEGSCLAFMRASSVLKSLPFPITSMKD
TEGIPCLGDKVKSIIEGIIEDGESSEAKAVLNDERYKSFKLFTSVFGVGLKTAEKWFRMGFRTLSKIQSD
KSLRFTQMQKAGFLYYEDLVSCVNRPEAEAVSMLVKEAVVTFLPDALVTMTGGFRRGKMTGHDVDFLITS
PEATEDEEQQLLHKVTDFWKQQGLLLYCDILESTFEKFKQPSRKVDALDHFQKCFLILKLDHGRVHSEKS
GQQEGKGWKAIRVDLVMCPYDRRAFALLGWTGSRQFERDLRRYATHERKMMLDNHALYDRTKGKTVTISP
LDGKVSKLQKALRVFLEAESEEEIFAHLGLDYIEPWERNA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_009345
ORF Size 1590 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_009345.2, NP_033371.2
RefSeq Size 2133 bp
RefSeq ORF 1593 bp
Locus ID 21673
UniProt ID P09838
Cytogenetics 19 34.32 cM
MW 60.8 kDa
Gene Summary Isoform TDT-S: Transferase that catalyzes the nontemplated addition of nucleoside triphosphate to coding ends during V(D)J recombination (N addition). Involved in the generation of diversity in the antigen-binding region of immunoglobulin heavy and light chains and T-cell receptors during B-and T-cell development. Does not act on double-stranded DNA with blunt ends.[UniProtKB/Swiss-Prot Function]

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