Dntt (NM_009345) Mouse Untagged Clone

CAT#: MC217581

Dntt (untagged) - Mouse deoxynucleotidyltransferase, terminal (Dntt), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dntt
• Synonyms: Tdt
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217581 representing NM_009345
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCCACTGCAAGCAGTCCACCTGGGTCCTCGGAAGAAAAGACCCAGGCAGCTGGGCACCCCAGTGG
  CTTCCACGCCTTATGACATCCGATTTCGAGACTTGGTCCTCTTCATTTTGGAAAAGAAGATGGGAACAAC
  TCGAAGAGCCTTCCTCATGGAGCTGGCCCGAAGGAAAGGGTTCAGGGTGGAAAATGAGCTCAGTGATTCG
  GTCACCCACATTGTGGCAGAGAACAACTCAGGGTCAGACGTCCTGGAGTGGCTCCAGCTGCAGAACATCA
  AAGCCAGCTCCGAGCTCGAACTCTTGGACATCTCCTGGCTCATCGAATGCATGGGAGCTGGGAAGCCAGT
  GGAGATGATGGGGAGACATCAGCTTGTTGTGAATAGAAACTCCTCCCCGAGTCCTGTCCCAGGCTCCCAG
  AATGTTCCAGCACCTGCTGTGAAGAAGATCTCCCAATATGCTTGCCAGCGAAGAACCACATTAAACAATT
  ACAACCAACTATTCACGGATGCCCTTGATATCCTGGCTGAAAATGATGAGCTTAGAGAGAATGAAGGTTC
  TTGCCTGGCATTCATGCGAGCGTCCTCTGTACTGAAATCTCTGCCATTCCCCATCACCAGCATGAAAGAC
  ACAGAGGGGATTCCTTGCCTAGGGGACAAGGTGAAGAGTATCATAGAGGGAATTATTGAAGATGGAGAAA
  GTTCTGAAGCTAAAGCTGTGTTAAATGATGAGCGATATAAATCCTTCAAACTCTTTACCTCTGTGTTTGG
  TGTGGGACTGAAGACAGCTGAGAAATGGTTCAGGATGGGTTTCAGAACTCTCAGCAAAATACAATCAGAC
  AAAAGCCTGAGGTTTACACAAATGCAGAAAGCAGGATTCCTCTACTACGAAGACCTCGTTAGCTGTGTGA
  ACAGGCCAGAAGCCGAGGCTGTCAGCATGCTAGTTAAGGAGGCGGTTGTGACATTTCTTCCAGATGCCTT
  GGTCACCATGACTGGGGGGTTCCGCAGGGGTAAGATGACTGGACATGATGTAGACTTTCTAATTACCAGC
  CCAGAAGCCACAGAGGATGAAGAGCAGCAGCTCTTGCATAAAGTGACAGACTTTTGGAAGCAGCAGGGGT
  TGCTTTTGTACTGCGACATCTTAGAGTCAACCTTTGAAAAGTTCAAGCAGCCCAGCAGGAAGGTGGATGC
  TCTCGACCATTTCCAGAAATGCTTCCTGATTCTGAAGCTGGACCACGGGAGAGTGCACAGCGAGAAGAGC
  GGCCAGCAGGAAGGAAAGGGCTGGAAGGCCATCCGTGTAGATCTGGTCATGTGCCCCTATGATCGCCGGG
  CCTTCGCCCTGCTTGGATGGACCGGCTCCAGACAGTTTGAGAGAGACTTGCGGCGCTATGCCACACACGA
  GAGGAAGATGATGCTGGACAACCACGCCCTGTATGACAGGACTAAGGGGAAGACTGTCACGATTTCACCT
  TTGGATGGGAAAGTGTCAAAACTACAGAAAGCCTTGAGGGTGTTTCTTGAAGCAGAAAGTGAAGAAGAGA
  TCTTTGCCCATCTGGGACTGGACTACATTGAACCATGGGAAAGAAATGCCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009345
• Insert Size: 1593 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009345.2, NP_033371.2
• RefSeq Size: 2133 bp
• RefSeq ORF: 1593 bp
• Locus ID: 21673
• UniProt ID: P09838
• Cytogenetics: 19 34.32 cM
• Gene Summary: Isoform TDT-S: Transferase that catalyzes the nontemplated addition of nucleoside triphosphate to coding ends during V(D)J recombination (N addition). Involved in the generation of diversity in the antigen-binding region of immunoglobulin heavy and light chains and T-cell receptors during B-and T-cell development. Does not act on double-stranded DNA with blunt ends.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).