Mtif3 (NM_029581) Mouse Tagged ORF Clone
CAT#: MR220522
- TrueORF®
Mtif3 (Myc-DDK-tagged) - Mouse mitochondrial translational initiation factor 3 (Mtif3), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_029581" in other vectors (4)
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USD 198.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Mtif3 |
Synonyms | 2810012L14Rik; AI414549 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR220522 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGTTCTTCTGATGAGGTTGATGCTACAAACTACAAAGCTTGACCATAACTTAATTGGAAGATGTT TGCAAAGACACGCTGTGAAGCCAGATCCAGCACAGCTGTCCCTGTCTGCGTCCACCCCAAAGCTGTTGTA CCTGACTTCTGCAAAAGGTTTTAGTACTGCTGGAGACCCACAGGGTGAGAGGAAACAGAAAAGAAGGGAT GCTTTCTCTAACACCGGCCGGAAGATTAGTGAGCGGATTATCCGCGTCCTGGATGAGAAGGGCATGGACC TGGGGATGATGCATCGAGCAGATGTGATCAGACTCATGAACAAGCAGGACCTGAGGCTGGTGCAGAGGAA CACCAGCTCAGAGCCACCGGAGTACCAGCTTATGACCGGAGAGCAGATCCACCAGGAGCGGCTAAAGCTC CGGGAACAGGAAAAGGCCAAACCCAAAACTGGACCAACTATGACGAAAGAGCTCGTTTTCTCTTCAAATA TTGGACAGCACGATTTAGACACCAAGAGTAAACAGATCCAGCAGTGGATAGAGAAGAAATACCACGTTCA AGTCACGATAAAGAGGAGGAAAGATGCGGAGCAGTCAGAGGAGGAAACGGAGGAGATATTTAACCAGATT CTCCAGACTATGCCTGACATAGCAACCTTCTCAAGCAGACCAAAAGCCATCAGAGGAGGGACTGCTTCCA TGTGTGTTTTTCGTCACTTGAGCAAGAAAGAAGAAAAGGCGTACAGAGAATCCCAGGAGAGCCAGAGAAG AGACACTTTGAGCAAAGACGACGACGGAAACAGCAAAGAGTCGGATGTTGTGTGTCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR220522 protein sequence
Red=Cloning site Green=Tags(s) MAVLLMRLMLQTTKLDHNLIGRCLQRHAVKPDPAQLSLSASTPKLLYLTSAKGFSTAGDPQGERKQKRRD AFSNTGRKISERIIRVLDEKGMDLGMMHRADVIRLMNKQDLRLVQRNTSSEPPEYQLMTGEQIHQERLKL REQEKAKPKTGPTMTKELVFSSNIGQHDLDTKSKQIQQWIEKKYHVQVTIKRRKDAEQSEEETEEIFNQI LQTMPDIATFSSRPKAIRGGTASMCVFRHLSKKEEKAYRESQESQRRDTLSKDDDGNSKESDVVCQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_029581 |
ORF Size | 831 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_029581.4, NP_083857.1 |
RefSeq Size | 4979 bp |
RefSeq ORF | 831 bp |
Locus ID | 76366 |
UniProt ID | Q9CZD5 |
Cytogenetics | 5 G3 |
MW | 31.7 kDa |
Gene Summary | IF-3 binds to the 28S ribosomal subunit and shifts the equilibrum between 55S ribosomes and their 39S and 28S subunits in favor of the free subunits, thus enhancing the availability of 28S subunits on which protein synthesis initiation begins.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC211722 | Mtif3 (untagged) - Mouse mitochondrial translational initiation factor 3 (Mtif3), nuclear gene encoding mitochondrial protein, (10ug) |
USD 330.00 |
|
MG220522 | Mtif3 (tGFP-tagged) - Mouse mitochondrial translational initiation factor 3 (Mtif3) nuclear gene encoding mitochondrial protein, (10ug) |
USD 500.00 |
|
MR220522L3 | Lenti ORF clone of Mtif3 (Myc-DDK-tagged) - Mouse mitochondrial translational initiation factor 3 (Mtif3), nuclear gene encoding mitochondrial protein |
USD 600.00 |
|
MR220522L4 | Lenti ORF clone of Mtif3 (mGFP-tagged) - Mouse mitochondrial translational initiation factor 3 (Mtif3), nuclear gene encoding mitochondrial protein |
USD 600.00 |
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