Mtif3 (NM_029581) Mouse Tagged ORF Clone

CAT#: MG220522

Mtif3 (tGFP-tagged) - Mouse mitochondrial translational initiation factor 3 (Mtif3) nuclear gene encoding mitochondrial protein, (10ug)

Product Images

Circular map for MG220522

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Mtif3
• Synonyms: 2810012L14Rik; AI414549
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG220522 representing NM_029581
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGTTCTTCTGATGAGGTTGATGCTACAAACTACAAAGCTTGACCATAACTTAATTGGAAGATGTT
  TGCAAAGACACGCTGTGAAGCCAGATCCAGCACAGCTGTCCCTGTCTGCGTCCACCCCAAAGCTGTTGTA
  CCTGACTTCTGCAAAAGGTTTTAGTACTGCTGGAGACCCACAGGGTGAGAGGAAACAGAAAAGAAGGGAT
  GCTTTCTCTAACACCGGCCGGAAGATTAGTGAGCGGATTATCCGCGTCCTGGATGAGAAGGGCATGGACC
  TGGGGATGATGCATCGAGCAGATGTGATCAGACTCATGAACAAGCAGGACCTGAGGCTGGTGCAGAGGAA
  CACCAGCTCAGAGCCACCGGAGTACCAGCTTATGACCGGAGAGCAGATCCACCAGGAGCGGCTAAAGCTC
  CGGGAACAGGAAAAGGCCAAACCCAAAACTGGACCAACTATGACGAAAGAGCTCGTTTTCTCTTCAAATA
  TTGGACAGCACGATTTAGACACCAAGAGTAAACAGATCCAGCAGTGGATAGAGAAGAAATACCACGTTCA
  AGTCACGATAAAGAGGAGGAAAGATGCGGAGCAGTCAGAGGAGGAAACGGAGGAGATATTTAACCAGATT
  CTCCAGACTATGCCTGACATAGCAACCTTCTCAAGCAGACCAAAAGCCATCAGAGGAGGGACTGCTTCCA
  TGTGTGTTTTTCGTCACTTGAGCAAGAAAGAAGAAAAGGCGTACAGAGAATCCCAGGAGAGCCAGAGAAG
  AGACACTTTGAGCAAAGACGACGACGGAAACAGCAAAGAGTCGGATGTTGTGTGTCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG220522 representing NM_029581
  Red=Cloning site Green=Tags(s)
  
  MAVLLMRLMLQTTKLDHNLIGRCLQRHAVKPDPAQLSLSASTPKLLYLTSAKGFSTAGDPQGERKQKRRD
  AFSNTGRKISERIIRVLDEKGMDLGMMHRADVIRLMNKQDLRLVQRNTSSEPPEYQLMTGEQIHQERLKL
  REQEKAKPKTGPTMTKELVFSSNIGQHDLDTKSKQIQQWIEKKYHVQVTIKRRKDAEQSEEETEEIFNQI
  LQTMPDIATFSSRPKAIRGGTASMCVFRHLSKKEEKAYRESQESQRRDTLSKDDDGNSKESDVVCQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_029581
• ORF Size: 828 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029581.4, NP_083857.1
• RefSeq Size: 1967 bp
• RefSeq ORF: 831 bp
• Locus ID: 76366
• UniProt ID: Q9CZD5
• Cytogenetics: 5 G3
• Gene Summary: IF-3 binds to the 28S ribosomal subunit and shifts the equilibrum between 55S ribosomes and their 39S and 28S subunits in favor of the free subunits, thus enhancing the availability of 28S subunits on which protein synthesis initiation begins.[UniProtKB/Swiss-Prot Function]