Acvr1b (NM_007395) Mouse Tagged ORF Clone

CAT#: MR220419

Acvr1b (Myc-DDK-tagged) - Mouse activin A receptor, type 1B (Acvr1b)

Product Images

Circular map for MR220419

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Acvr1b
• Synonyms: 6820432J04; ActR-IB; ActRIB; Acvrlk4; Alk4; SKR2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220419 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGTCGGCCGGAGCCTCCTCCTTCTTCCCCCTTGTTGTCCTCCTGCTCGCCGGCAGCGGCGGGT
  CCGGGCCCCGGGGGATCCAGGCTCTGCTGTGTGCGTGCACCAGCTGCCTACAGACCAACTACACCTGTGA
  GACAGATGGGGCTTGCATGGTCTCCATCTTTAACCTGGATGGCGTGGAGCACCATGTACGTACCTGCATC
  CCCAAGGTGGAGCTGGTTCCTGCTGGAAAGCCCTTCTACTGCCTGAGTTCAGAGGATCTGCGCAACACAC
  ACTGCTGCTATATTGACTTCTGCAACAAGATTGACCTCAGGGTCCCCAGCGGACACCTCAAGGAGCCTGC
  GCACCCCTCCATGTGGGGCCCTGTGGAGCTGGTCGGCATCATCGCCGGCCCCGTCTTCCTCCTCTTCCTT
  ATCATTATCATCGTCTTCCTGGTCATCAACTATCACCAGCGTGTCTACCATAACCGCCAGAGGTTGGACA
  TGGAGGACCCCTCTTGCGAGATGTGTCTCTCCAAAGACAAGACGCTCCAGGATCTCGTCTACGACCTCTC
  CACGTCAGGGTCTGGCTCAGGGTTACCCCTTTTTGTCCAGCGCACAGTGGCCCGAACCATTGTTTTACAA
  GAGATTATCGGCAAGGGCCGGTTCGGGGAAGTATGGCGTGGTCGCTGGAGGGGTGGTGACGTGGCTGTGA
  AAATCTTCTCTTCTCGTGAAGAACGGTCTTGGTTCCGTGAAGCAGAGATCTACCAGACCGTCATGCTGCG
  CCATGAAAACATCCTTGGCTTTATTGCTGCTGACAATAAAGATAATGGCACCTGGACCCAGCTGTGGCTT
  GTCTCTGACTATCACGAGCATGGCTCACTGTTTGATTATCTGAACCGCTACACAGTGACCATTGAGGGAA
  TGATTAAGCTAGCCTTGTCTGCAGCCAGTGGTTTGGCACACCTGCATATGGAGATTGTGGGCACTCAAGG
  GAAGCCGGGAATTGCTCATCGAGACTTGAAGTCAAAGAACATCCTGGTGAAAAAAAATGGCATGTGTGCC
  ATTGCAGACCTGGGCCTGGCTGTCCGTCATGATGCGGTCACTGACACCATAGACATTGCTCCAAATCAGA
  GGGTGGGGACCAAACGATACATGGCTCCTGAAGTCCTTGACGAGACAATCAACATGAAGCACTTTGACTC
  CTTCAAATGTGCCGACATCTATGCCCTCGGGCTTGTCTACTGGGAGATTGCACGAAGATGCAATTCTGGA
  GGAGTCCATGAAGACTATCAACTGCCGTATTACGACTTAGTGCCCTCCGACCCTTCCATTGAGGAGATGC
  GAAAGGTTGTATGTGACCAGAAGCTACGGCCCAATGTCCCCAACTGGTGGCAGAGTTATGAGGCCTTGCG
  AGTGATGGGAAAGATGATGCGGGAGTGCTGGTACGCCAATGGTGCTGCCCGTCTGACAGCTCTGCGCATC
  AAGAAGACTCTGTCCCAGCTAAGCGTGCAGGAAGATGTGAAGATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220419 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAESAGASSFFPLVVLLLAGSGGSGPRGIQALLCACTSCLQTNYTCETDGACMVSIFNLDGVEHHVRTCI
  PKVELVPAGKPFYCLSSEDLRNTHCCYIDFCNKIDLRVPSGHLKEPAHPSMWGPVELVGIIAGPVFLLFL
  IIIIVFLVINYHQRVYHNRQRLDMEDPSCEMCLSKDKTLQDLVYDLSTSGSGSGLPLFVQRTVARTIVLQ
  EIIGKGRFGEVWRGRWRGGDVAVKIFSSREERSWFREAEIYQTVMLRHENILGFIAADNKDNGTWTQLWL
  VSDYHEHGSLFDYLNRYTVTIEGMIKLALSAASGLAHLHMEIVGTQGKPGIAHRDLKSKNILVKKNGMCA
  IADLGLAVRHDAVTDTIDIAPNQRVGTKRYMAPEVLDETINMKHFDSFKCADIYALGLVYWEIARRCNSG
  GVHEDYQLPYYDLVPSDPSIEEMRKVVCDQKLRPNVPNWWQSYEALRVMGKMMRECWYANGAARLTALRI
  KKTLSQLSVQEDVKI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007395
• ORF Size: 1518 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007395.4
• RefSeq Size: 3277 bp
• RefSeq ORF: 1518 bp
• Locus ID: 11479
• UniProt ID: Q61271
• Cytogenetics: 15 56.48 cM
• MW: 56.7 kDa
• Gene Summary: Transmembrane serine/threonine kinase activin type-1 receptor forming an activin receptor complex with activin receptor type-2 (ACVR2A or ACVR2B). Transduces the activin signal from the cell surface to the cytoplasm and is thus regulating a many physiological and pathological processes including neuronal differentiation and neuronal survival, hair follicle development and cycling, FSH production by the pituitary gland, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. Activin is also thought to have a paracrine or autocrine role in follicular development in the ovary. Within the receptor complex, type-2 receptors (ACVR2A and/or ACVR2B) act as a primary activin receptors whereas the type-1 receptors like ACVR1B act as downstream transducers of activin signals. Activin binds to type-2 receptor at the plasma membrane and activates its serine-threonine kinase. The activated receptor type-2 then phosphorylates and activates the type-1 receptor such as ACVR1B. Once activated, the type-1 receptor binds and phosphorylates the SMAD proteins SMAD2 and SMAD3, on serine residues of the C-terminal tail. Soon after their association with the activin receptor and subsequent phosphorylation, SMAD2 and SMAD3 are released into the cytoplasm where they interact with the common partner SMAD4. This SMAD complex translocates into the nucleus where it mediates activin-induced transcription. Inhibitory SMAD7, which is recruited to ACVR1B through FKBP1A, can prevent the association of SMAD2 and SMAD3 with the activin receptor complex, thereby blocking the activin signal. Activin signal transduction is also antagonized by the binding to the receptor of inhibin-B via the IGSF1 inhibin coreceptor. ACVR1B also phosphorylates TDP2.[UniProtKB/Swiss-Prot Function]