Acvr1b (NM_007395) Mouse Untagged Clone

CAT#: MC217130

Acvr1b (untagged) - Mouse activin A receptor, type 1B (Acvr1b), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Acvr1b
• Synonyms: 6820432J04; ActR-IB; ActRIB; Acvrlk4; Alk4; SKR2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217130 representing NM_007395
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGTCGGCCGGAGCCTCCTCCTTCTTCCCCCTTGTTGTCCTCCTGCTCGCCGGCAGCGGCGGGT
  CCGGGCCCCGGGGGATCCAGGCTCTGCTGTGTGCGTGCACCAGCTGCCTACAGACCAACTACACCTGTGA
  GACAGATGGGGCTTGCATGGTCTCCATCTTTAACCTGGATGGCGTGGAGCACCATGTACGTACCTGCATC
  CCCAAGGTGGAGCTGGTTCCTGCTGGAAAGCCCTTCTACTGCCTGAGTTCAGAGGATCTGCGCAACACAC
  ACTGCTGCTATATTGACTTCTGCAACAAGATTGACCTCAGGGTCCCCAGCGGACACCTCAAGGAGCCTGC
  GCACCCCTCCATGTGGGGCCCTGTGGAGCTGGTCGGCATCATCGCCGGCCCCGTCTTCCTCCTCTTCCTT
  ATCATTATCATCGTCTTCCTGGTCATCAACTATCACCAGCGTGTCTACCATAACCGCCAGAGGTTGGACA
  TGGAGGACCCCTCTTGCGAGATGTGTCTCTCCAAAGACAAGACGCTCCAGGATCTCGTCTACGACCTCTC
  CACGTCAGGGTCTGGCTCAGGGTTACCCCTTTTTGTCCAGCGCACAGTGGCCCGAACCATTGTTTTACAA
  GAGATTATCGGCAAGGGCCGGTTCGGGGAAGTATGGCGTGGTCGCTGGAGGGGTGGTGACGTGGCTGTGA
  AAATCTTCTCTTCTCGTGAAGAACGGTCTTGGTTCCGTGAAGCAGAGATCTACCAGACCGTCATGCTGCG
  CCATGAAAACATCCTTGGCTTTATTGCTGCTGACAATAAAGATAATGGCACCTGGACCCAGCTGTGGCTT
  GTCTCTGACTATCACGAGCATGGCTCACTGTTTGATTATCTGAACCGCTACACAGTGACCATTGAGGGAA
  TGATTAAGCTAGCCTTGTCTGCAGCCAGTGGTTTGGCACACCTGCATATGGAGATTGTGGGCACTCAAGG
  GAAGCCGGGAATTGCTCATCGAGACTTGAAGTCAAAGAACATCCTGGTGAAAAAAAATGGCATGTGTGCC
  ATTGCAGACCTGGGCCTGGCTGTCCGTCATGATGCGGTCACTGACACCATAGACATTGCTCCAAATCAGA
  GGGTGGGGACCAAACGATACATGGCTCCTGAAGTCCTTGACGAGACAATCAACATGAAGCACTTTGACTC
  CTTCAAATGTGCCGACATCTATGCCCTCGGGCTTGTCTACTGGGAGATTGCACGAAGATGCAATTCTGGA
  GGAGTCCATGAAGACTATCAACTGCCGTATTACGACTTAGTGCCCTCCGACCCTTCCATTGAGGAGATGC
  GAAAGGTTGTATGTGACCAGAAGCTACGGCCCAATGTCCCCAACTGGTGGCAGAGTTATGAGGCCTTGCG
  AGTGATGGGAAAGATGATGCGGGAGTGCTGGTACGCCAATGGTGCTGCCCGTCTGACAGCTCTGCGCATC
  AAGAAGACTCTGTCCCAGCTAAGCGTGCAGGAAGATGTGAAGATTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_007395
• Insert Size: 1518 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007395.3, NP_031421.1
• RefSeq Size: 3277 bp
• RefSeq ORF: 1518 bp
• Locus ID: 11479
• UniProt ID: Q61271
• Cytogenetics: 15 56.48 cM
• Gene Summary: Transmembrane serine/threonine kinase activin type-1 receptor forming an activin receptor complex with activin receptor type-2 (ACVR2A or ACVR2B). Transduces the activin signal from the cell surface to the cytoplasm and is thus regulating a many physiological and pathological processes including neuronal differentiation and neuronal survival, hair follicle development and cycling, FSH production by the pituitary gland, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. Activin is also thought to have a paracrine or autocrine role in follicular development in the ovary. Within the receptor complex, type-2 receptors (ACVR2A and/or ACVR2B) act as a primary activin receptors whereas the type-1 receptors like ACVR1B act as downstream transducers of activin signals. Activin binds to type-2 receptor at the plasma membrane and activates its serine-threonine kinase. The activated receptor type-2 then phosphorylates and activates the type-1 receptor such as ACVR1B. Once activated, the type-1 receptor binds and phosphorylates the SMAD proteins SMAD2 and SMAD3, on serine residues of the C-terminal tail. Soon after their association with the activin receptor and subsequent phosphorylation, SMAD2 and SMAD3 are released into the cytoplasm where they interact with the common partner SMAD4. This SMAD complex translocates into the nucleus where it mediates activin-induced transcription. Inhibitory SMAD7, which is recruited to ACVR1B through FKBP1A, can prevent the association of SMAD2 and SMAD3 with the activin receptor complex, thereby blocking the activin signal. Activin signal transduction is also antagonized by the binding to the receptor of inhibin-B via the IGSF1 inhibin coreceptor. ACVR1B also phosphorylates TDP2.[UniProtKB/Swiss-Prot Function]