Il10ra (NM_008348) Mouse Tagged ORF Clone

CAT#: MR219426

Il10ra (Myc-DDK-tagged) - Mouse interleukin 10 receptor, alpha (Il10ra)

Product Images

Circular map for MR219426

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Il10ra
• Synonyms: AW553859; CDw210; CDw210a; IL-10R1; IL-10RA; Il10r; mIL-10R
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR219426 representing NM_008348
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGTCGCGTTTGCTCCCATTCCTCGTCACGATCTCCAGCCTGAGCCTAGAATTCATTGCATACGGGA
  CAGAACTGCCAAGCCCTTCCTATGTGTGGTTTGAAGCCAGATTTTTCCAGCACATCCTCCACTGGAAACC
  TATCCCAAACCAGTCTGAGAGCACCTACTATGAAGTGGCCCTCAAACAGTACGGAAACTCAACCTGGAAT
  GACATCCATATCTGTAGAAAGGCTCAGGCATTGTCCTGTGATCTCACAACGTTCACCCTGGATCTGTATC
  ACCGAAGCTATGGCTACCGGGCCAGAGTCCGGGCAGTGGACAACAGTCAGTACTCCAACTGGACCACCAC
  TGAGACTCGCTTCACAGTGGATGAAGTGATTCTGACAGTGGATAGCGTGACTCTGAAAGCAATGGACGGC
  ATCATCTATGGGACAATCCATCCCCCCAGGCCCACGATAACCCCTGCAGGGGATGAGTACGAACAAGTCT
  TCAAGGATCTCCGAGTTTACAAGATTTCCATCCGGAAGTTCTCAGAACTAAAGAATGCAACCAAGAGAGT
  GAAACAGGAAACCTTCACCCTCACGGTCCCCATAGGGGTGAGAAAGTTTTGTGTCAAGGTGCTGCCCCGC
  TTGGAATCCCGAATTAACAAGGCAGAGTGGTCGGAGGAGCAGTGTTTACTTATCACGACGGAGCAGTATT
  TCACTGTGACCAACCTGAGCATCTTAGTCATATCTATGCTGCTATTCTGTGGAATCCTGGTCTGTCTGGT
  TCTCCAGTGGTACATCCGGCACCCGGGGAAGTTGCCTACAGTCCTGGTCTTCAAGAAGCCTCACGACTTC
  TTCCCAGCCAACCCTCTCTGCCCAGAAACTCCCGATGCCATTCACATCGTGGACCTGGAGGTTTTCCCAA
  AGGTGTCACTAGAGCTGAGAGACTCAGTCCTGCATGGCAGCACCGACAGTGGCTTTGGCAGTGGTAAACC
  ATCACTTCAGACTGAAGAGTCCCAATTCCTCCTCCCTGGCTCCCACCCCCAGATACAGGGGACTCTGGGA
  AAAGAAGAGTCTCCAGGGCTACAGGCCACCTGTGGGGACAACACGGACAGTGGGATCTGCCTGCAGGAGC
  CCGGCTTACACTCCAGCATGGGGCCCGCCTGGAAGCAGCAGCTTGGATATACCCATCAGGACCAGGATGA
  CAGTGACGTTAACCTAGTCCAGAACTCTCCAGGGCAGCCTAAGTACACACAGGATGCATCTGCCTTGGGC
  CATGTCTGTCTCCTAGAACCTAAAGCCCCTGAGGAGAAAGACCAAGTCATGGTGACATTCCAGGGCTACC
  AGAAACAGACCAGATGGAAGGCAGAGGCAGCAGGCCCAGCAGAATGCTTGGACGAAGAGATTCCCTTGAC
  AGATGCCTTTGATCCTGAACTTGGGGTACACCTGCAGGATGATTTGGCTTGGCCTCCACCAGCTCTGGCC
  GCAGGTTATTTGAAACAGGAGTCTCAAGGGATGGCTTCTGCTCCACCAGGGACACCAAGTAGACAGTGGA
  ATCAACTGACCGAAGAGTGGTCACTCCTGGGTGTGGTTAGCTGTGAAGATCTAAGCATAGAAAGTTGGAG
  GTTTGCCCATAAACTTGACCCTCTGGACTGTGGGGCAGCCCCTGGTGGCCTCCTGGATAGCCTTGGCTCT
  AACCTGGTCACCCTGCCGTTGATCTCCAGCCTGCAGGTAGAAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR219426 representing NM_008348
  Red=Cloning site Green=Tags(s)
  
  MLSRLLPFLVTISSLSLEFIAYGTELPSPSYVWFEARFFQHILHWKPIPNQSESTYYEVALKQYGNSTWN
  DIHICRKAQALSCDLTTFTLDLYHRSYGYRARVRAVDNSQYSNWTTTETRFTVDEVILTVDSVTLKAMDG
  IIYGTIHPPRPTITPAGDEYEQVFKDLRVYKISIRKFSELKNATKRVKQETFTLTVPIGVRKFCVKVLPR
  LESRINKAEWSEEQCLLITTEQYFTVTNLSILVISMLLFCGILVCLVLQWYIRHPGKLPTVLVFKKPHDF
  FPANPLCPETPDAIHIVDLEVFPKVSLELRDSVLHGSTDSGFGSGKPSLQTEESQFLLPGSHPQIQGTLG
  KEESPGLQATCGDNTDSGICLQEPGLHSSMGPAWKQQLGYTHQDQDDSDVNLVQNSPGQPKYTQDASALG
  HVCLLEPKAPEEKDQVMVTFQGYQKQTRWKAEAAGPAECLDEEIPLTDAFDPELGVHLQDDLAWPPPALA
  AGYLKQESQGMASAPPGTPSRQWNQLTEEWSLLGVVSCEDLSIESWRFAHKLDPLDCGAAPGGLLDSLGS
  NLVTLPLISSLQVEE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008348
• ORF Size: 1725 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008348.3
• RefSeq Size: 3493 bp
• RefSeq ORF: 1728 bp
• Locus ID: 16154
• UniProt ID: Q61727
• Cytogenetics: 9 24.84 cM
• MW: 64.7 kDa
• Gene Summary: Cell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Upon binding to IL10, induces a conformational change in IL10RB, allowing IL10RB to bind IL10 as well. In turn, the heterotetrameric assembly complex, composed of two subunits of IL10RA and IL10RB, activates the kinases JAK1 and TYK2 that are constitutively associated with IL10RA and IL10RB respectively. These kinases then phosphorylate specific tyrosine residues in the intracellular domain in IL10RA leading to the recruitment and subsequent phosphorylation of STAT3 (PubMed:8910398). Once phosphorylated, STAT3 homodimerizes, translocates to the nucleus and activates the expression of anti-inflammatory genes. In addition, IL10RA-mediated activation of STAT3 inhibits starvation-induced autophagy (By similarity).[UniProtKB/Swiss-Prot Function]