Il10ra (NM_008348) Mouse Untagged Clone

CAT#: MC219161

Il10ra (untagged) - Mouse interleukin 10 receptor, alpha (Il10ra), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Il10ra
• Synonyms: AW553859; CDw210; CDw210a; IL-10R1; IL-10RA; Il10r; mIL-10R
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219161 representing NM_008348
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGTCGCGTTTGCTCCCATTCCTCGTCACGATCTCCAGCCTGAGCCTAGAATTCATTGCATACGGGA
  CAGAACTGCCAAGCCCTTCCTATGTGTGGTTTGAAGCCAGATTTTTCCAGCACATCCTCCACTGGAAACC
  TATCCCAAACCAGTCTGAGAGCACCTACTATGAAGTGGCCCTCAAACAGTACGGAAACTCAACCTGGAAT
  GACATCCATATCTGTAGAAAGGCTCAGGCATTGTCCTGTGATCTCACAACGTTCACCCTGGATCTGTATC
  ACCGAAGCTATGGCTACCGGGCCAGAGTCCGGGCAGTGGACAACAGTCAGTACTCCAACTGGACCACCAC
  TGAGACTCGCTTCACAGTGGATGAAGTGATTCTGACAGTGGATAGCGTGACTCTGAAAGCAATGGACGGC
  ATCATCTATGGGACAATCCATCCCCCCAGGCCCACGATAACCCCTGCAGGGGATGAGTACGAACAAGTCT
  TCAAGGATCTCCGAGTTTACAAGATTTCCATCCGGAAGTTCTCAGAACTAAAGAATGCAACCAAGAGAGT
  GAAACAGGAAACCTTCACCCTCACGGTCCCCATAGGGGTGAGAAAGTTTTGTGTCAAGGTGCTGCCCCGC
  TTGGAATCCCGAATTAACAAGGCAGAGTGGTCGGAGGAGCAGTGTTTACTTATCACGACGGAGCAGTATT
  TCACTGTGACCAACCTGAGCATCTTAGTCATATCTATGCTGCTATTCTGTGGAATCCTGGTCTGTCTGGT
  TCTCCAGTGGTACATCCGGCACCCGGGGAAGTTGCCTACAGTCCTGGTCTTCAAGAAGCCTCACGACTTC
  TTCCCAGCCAACCCTCTCTGCCCAGAAACTCCCGATGCCATTCACATCGTGGACCTGGAGGTTTTCCCAA
  AGGTGTCACTAGAGCTGAGAGACTCAGTCCTGCATGGCAGCACCGACAGTGGCTTTGGCAGTGGTAAACC
  ATCACTTCAGACTGAAGAGTCCCAATTCCTCCTCCCTGGCTCCCACCCCCAGATACAGGGGACTCTGGGA
  AAAGAAGAGTCTCCAGGGCTACAGGCCACCTGTGGGGACAACACGGACAGTGGGATCTGCCTGCAGGAGC
  CCGGCTTACACTCCAGCATGGGGCCCGCCTGGAAGCAGCAGCTTGGATATACCCATCAGGACCAGGATGA
  CAGTGACGTTAACCTAGTCCAGAACTCTCCAGGGCAGCCTAAGTACACACAGGATGCATCTGCCTTGGGC
  CATGTCTGTCTCCTAGAACCTAAAGCCCCTGAGGAGAAAGACCAAGTCATGGTGACATTCCAGGGCTACC
  AGAAACAGACCAGATGGAAGGCAGAGGCAGCAGGCCCAGCAGAATGCTTGGACGAAGAGATTCCCTTGAC
  AGATGCCTTTGATCCTGAACTTGGGGTACACCTGCAGGATGATTTGGCTTGGCCTCCACCAGCTCTGGCC
  GCAGGTTATTTGAAACAGGAGTCTCAAGGGATGGCTTCTGCTCCACCAGGGACACCAAGTAGACAGTGGA
  ATCAACTGACCGAAGAGTGGTCACTCCTGGGTGTGGTTAGCTGTGAAGATCTAAGCATAGAAAGTTGGAG
  GTTTGCCCATAAACTTGACCCTCTGGACTGTGGGGCAGCCCCTGGTGGCCTCCTGGATAGCCTTGGCTCT
  AACCTGGTCACCCTGCCGTTGATCTCCAGCCTGCAGGTAGAAGAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008348
• Insert Size: 1728 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008348.2, NP_032374.1
• RefSeq Size: 3493 bp
• RefSeq ORF: 1728 bp
• Locus ID: 16154
• UniProt ID: Q61727
• Cytogenetics: 9 24.84 cM
• Gene Summary: Cell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Upon binding to IL10, induces a conformational change in IL10RB, allowing IL10RB to bind IL10 as well. In turn, the heterotetrameric assembly complex, composed of two subunits of IL10RA and IL10RB, activates the kinases JAK1 and TYK2 that are constitutively associated with IL10RA and IL10RB respectively. These kinases then phosphorylate specific tyrosine residues in the intracellular domain in IL10RA leading to the recruitment and subsequent phosphorylation of STAT3 (PubMed:8910398). Once phosphorylated, STAT3 homodimerizes, translocates to the nucleus and activates the expression of anti-inflammatory genes. In addition, IL10RA-mediated activation of STAT3 inhibits starvation-induced autophagy (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (1).