Cd163 (NM_053094) Mouse Tagged ORF Clone

CAT#: MR219212

Cd163 (Myc-DDK-tagged) - Mouse CD163 antigen (Cd163), transcript variant 2

Product Images

Circular map for MR219212

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cd163
• Synonyms: CD163v2; CD163v3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR219212 representing NM_053094
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTGGACACAGAATGGTTCTTCTTGGAGGTGCTGGATCTCCTGGTTGTAAAAGGTTTGTCCATCTAG
  GTTTCTTTGTTGTGGCTGTGAGCTCACTTCTCAGTGCCTCTGCTGTCACTAACGCTCCTGGAGAAATGAA
  GAAGGAACTGAGACTGGCGGGTGGTGAAAACAACTGTAGTGGGAGAGTGGAACTTAAGATCCATGACAAG
  TGGGGCACAGTGTGCAGTAACGGCTGGAGCATGAATGAAGTGTCCGTGGTTTGCCAGCAGCTGGGATGCC
  CAACTTCTATTAAAGCCCTTGGATGGGCTAACTCCAGCGCCGGCTCTGGATATATCTGGATGGACAAAGT
  TTCTTGTACAGGGAATGAGTCAGCTCTTTGGGACTGCAAACATGATGGGTGGGGAAAGCATAACTGTACC
  CATGAAAAAGATGCTGGAGTGACCTGCTCAGATGGATCTAATTTGGAGATGAGACTGGTGAACAGTGCGG
  GCCACCGATGCTTAGGAAGAGTAGAAATAAAGTTCCAGGGAAAGTGGGGGACGGTGTGTGACGACAACTT
  CAGCAAAGATCACGCTTCTGTGATTTGTAAACAGCTTGGATGTGGAAGTGCCATTAGTTTCTCTGGCTCA
  GCTAAATTGGGAGCTGGTTCTGGACCAATCTGGCTCGATGACCTGGCATGCAATGGAAATGAGTCAGCTC
  TCTGGGACTGCAAACACCGGGGATGGGGCAAGCATAACTGTGACCATGCTGAGGATGTCGGTGTGATTTG
  CTTAGAGGGAGCAGATCTGAGCCTGAGACTAGTGGATGGAGTGTCCAGATGTTCAGGAAGATTGGAAGTG
  AGATTCCAAGGAGAATGGGGGACCGTGTGTGATGATAACTGGGATCTCCGGGATGCTTCTGTGGTGTGCA
  AGCAACTGGGATGTCCAACTGCCATCAGTGCCATTGGTCGAGTTAATGCCAGTGAGGGATCTGGACAGAT
  TTGGCTTGACAACATTTCATGCGAAGGACATGAGGCAACTCTTTGGGAGTGTAAACACCAAGAGTGGGGA
  AAGCATTACTGTCATCATAGAGAAGACGCTGGCGTGACATGTTCTGATGGAGCAGATCTGGAACTTAGAC
  TTGTAGGTGGAGGCAGTCGCTGTGCTGGCATTGTGGAGGTGGAGATTCAGAAGCTGACTGGGAAGATGTG
  TAGCCGAGGCTGGACACTGGCAGATGCGGATGTGGTTTGCAGACAGCTTGGATGTGGATCTGCGCTTCAA
  ACCCAGGCTAAGATCTACTCTAAAACTGGGGCAACAAATACGTGGCTCTTTCCTGGATCTTGTAATGGAA
  ATGAAACTACTTTTTGGCAATGCAAAAACTGGCAGTGGGGCGGCCTTTCCTGTGATAATTTCGAAGAAGC
  CAAAGTTACCTGCTCAGGCCACAGGGAACCCAGACTGGTTGGAGGAGAAATCCCATGCTCTGGTCGTGTG
  GAAGTGAAACACGGAGACGTGTGGGGCTCCGTCTGTGATTTTGACTTGTCTCTGGAAGCTGCCAGTGTGG
  TGTGCAGGGAATTACAATGTGGAACAGTCGTCTCTATCCTAGGGGGAGCACATTTTGGAGAAGGAAGTGG
  ACAGATCTGGGGTGAAGAATTCCAGTGTAGTGGGGATGAGTCCCATCTTTCACTATGCTCAGTGGCGCCC
  CCGCTAGACAGAACTTGTACCCACAGCAGGGATGTCAGCGTAGTCTGCTCACGATACATAGATATTCGTC
  TGGCAGGCGGCGAGTCCTCCTGTGAGGGAAGAGTGGAGCTCAAGACACTCGGAGCCTGGGGTCCCCTCTG
  CAGTTCTCATTGGGACATGGAAGATGCTCATGTCTTATGTCAGCAGCTGAAGTGTGGGGTTGCCCAATCT
  ATTCCAGAAGGAGCACATTTTGGGAAAGGAGCTGGTCAGGTCTGGAGTCACATGTTCCACTGCACTGGAA
  CTGAGGAACATATAGGAGATTGCCTCATGACTGCTCTGGGTGCGCCGACGTGTTCCGAAGGACAGGTGGC
  CTCTGTCATCTGCTCAGGAAACCAATCCCAGACACTATTGCCATGTAGTTCATTGTCTCCAGTCCAAACA
  ACAAGCTCTACAATTCCAAAGGAGAGTGAAGTTCCCTGCATAGCAAGTGGCCAGCTTCGCTTGGTAGGTG
  GAGGTGGTCGCTGCGCTGGAAGAGTGGAGGTCTACCACGAGGGCTCTTGGGGCACCGTCTGTGATGACAA
  TTGGGATATGACTGATGCCAATGTGGTGTGCAAGCAGCTGGACTGTGGCGTGGCAATTAACGCCACTGGC
  TCTGCTTACTTCGGGGAAGGAGCAGGAGCTATCTGGCTAGACGAAGTCATCTGCACTGGGAAAGAGTCTC
  ATATTTGGCAGTGCCATTCACATGGCTGGGGACGCCATAACTGCAGGCACAAAGAAGATGCAGGTGTTAT
  CTGCTCCGAGTTCATGTCTCTGAGGCTGACCAACGAAGCCCACAAAGAAAACTGCACAGGTCGCCTTGAA
  GTGTTTTACAATGGTACATGGGGCAGTATTGGCAGTAGCAATATGTCTCCAACCACTGTGGGGGTGGTGT
  GCCGTCAGCTGGGCTGTGCAGACAACGGGACTGTGAAACCCATACCTTCAGACAAGACACCATCCAGGCC
  CATGTGGGTAGATCGTGTGCAGTGTCCAAAAGGAGTTGACACTTTGTGGCAGTGCCCCTCGTCACCTTGG
  AAACAGAGACAGGCCAGCCCCTCCTCCCAGGAGTCCTGGATCATCTGTGACAACAAAATAAGACTCCAGG
  AAGGGCATACAGACTGTTCTGGACGTGTGGAGATCTGGCACAAAGGTTCCTGGGGAACAGTGTGTGATGA
  CTCCTGGGATCTTAATGATGCTAAGGTTGTATGTAAGCAGTTGGGCTGTGGCCAAGCTGTGAAGGCACTA
  AAAGAAGCAGCATTTGGTCCAGGAACTGGGCCCATATGGCTCAATGAAATTAAGTGTAGAGGGAATGAGT
  CTTCCCTGTGGGATTGTCCTGCCAAACCGTGGAGTCACAGCGACTGTGGGCACAAAGAAGATGCTTCCAT
  CCAGTGCCTCCCAAAAATGACTTCAGAATCACATCATGGCACAGGTCACCCCACCCTCACGGCACTCTTG
  GTTTGTGGAGCCATTCTATTGGTCCTCCTCATTGTCTTCCTCCTGTGGACTCTGAAGCGACGACAGATTC
  AGCGACTTACAGTTTCCTCAAGAGGAGAGGTCTTGATACATCAAGTTCAGTACCAAGAGATGGATTCAAA
  GGCGGATGATCTGGACTTGCTGAAATCCTCGGGGGTCATTCAGAGGCACACTGAGAAGGAAAATGATAAT
  TTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR219212 representing NM_053094
  Red=Cloning site Green=Tags(s)
  
  MGGHRMVLLGGAGSPGCKRFVHLGFFVVAVSSLLSASAVTNAPGEMKKELRLAGGENNCSGRVELKIHDK
  WGTVCSNGWSMNEVSVVCQQLGCPTSIKALGWANSSAGSGYIWMDKVSCTGNESALWDCKHDGWGKHNCT
  HEKDAGVTCSDGSNLEMRLVNSAGHRCLGRVEIKFQGKWGTVCDDNFSKDHASVICKQLGCGSAISFSGS
  AKLGAGSGPIWLDDLACNGNESALWDCKHRGWGKHNCDHAEDVGVICLEGADLSLRLVDGVSRCSGRLEV
  RFQGEWGTVCDDNWDLRDASVVCKQLGCPTAISAIGRVNASEGSGQIWLDNISCEGHEATLWECKHQEWG
  KHYCHHREDAGVTCSDGADLELRLVGGGSRCAGIVEVEIQKLTGKMCSRGWTLADADVVCRQLGCGSALQ
  TQAKIYSKTGATNTWLFPGSCNGNETTFWQCKNWQWGGLSCDNFEEAKVTCSGHREPRLVGGEIPCSGRV
  EVKHGDVWGSVCDFDLSLEAASVVCRELQCGTVVSILGGAHFGEGSGQIWGEEFQCSGDESHLSLCSVAP
  PLDRTCTHSRDVSVVCSRYIDIRLAGGESSCEGRVELKTLGAWGPLCSSHWDMEDAHVLCQQLKCGVAQS
  IPEGAHFGKGAGQVWSHMFHCTGTEEHIGDCLMTALGAPTCSEGQVASVICSGNQSQTLLPCSSLSPVQT
  TSSTIPKESEVPCIASGQLRLVGGGGRCAGRVEVYHEGSWGTVCDDNWDMTDANVVCKQLDCGVAINATG
  SAYFGEGAGAIWLDEVICTGKESHIWQCHSHGWGRHNCRHKEDAGVICSEFMSLRLTNEAHKENCTGRLE
  VFYNGTWGSIGSSNMSPTTVGVVCRQLGCADNGTVKPIPSDKTPSRPMWVDRVQCPKGVDTLWQCPSSPW
  KQRQASPSSQESWIICDNKIRLQEGHTDCSGRVEIWHKGSWGTVCDDSWDLNDAKVVCKQLGCGQAVKAL
  KEAAFGPGTGPIWLNEIKCRGNESSLWDCPAKPWSHSDCGHKEDASIQCLPKMTSESHHGTGHPTLTALL
  VCGAILLVLLIVFLLWTLKRRQIQRLTVSSRGEVLIHQVQYQEMDSKADDLDLLKSSGVIQRHTEKENDN
  L
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053094
• ORF Size: 3363 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053094.1, NM_053094.2, NP_444324.2
• RefSeq Size: 4409 bp
• RefSeq ORF: 3366 bp
• Locus ID: 93671
• UniProt ID: Q2VLH6
• Cytogenetics: 6 F2
• MW: 121.4 kDa
• Gene Summary: Involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1 (By similarity).[UniProtKB/Swiss-Prot Function]