Cd163 (NM_053094) Mouse Untagged Clone

CAT#: MC223654

Cd163 (untagged) - Mouse CD163 antigen (Cd163), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cd163
• Synonyms: CD163v2; CD163v3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223654 representing NM_053094
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTGGACACAGAATGGTTCTTCTTGGAGGTGCTGGATCTCCTGGTTGTAAAAGGTTTGTCCATCTAG
  GTTTCTTTGTTGTGGCTGTGAGCTCACTTCTCAGTGCCTCTGCTGTCACTAACGCTCCTGGAGAAATGAA
  GAAGGAACTGAGACTGGCGGGTGGTGAAAACAACTGTAGTGGGAGAGTGGAACTTAAGATCCATGACAAG
  TGGGGCACAGTGTGCAGTAACGGCTGGAGCATGAATGAAGTGTCCGTGGTTTGCCAGCAGCTGGGATGCC
  CAACTTCTATTAAAGCCCTTGGATGGGCTAACTCCAGCGCCGGCTCTGGATATATCTGGATGGACAAAGT
  TTCTTGTACAGGGAATGAGTCAGCTCTTTGGGACTGCAAACATGATGGGTGGGGAAAGCATAACTGTACC
  CATGAAAAAGATGCTGGAGTGACCTGCTCAGATGGATCTAATTTGGAGATGAGACTGGTGAACAGTGCGG
  GCCACCGATGCTTAGGAAGAGTAGAAATAAAGTTCCAGGGAAAGTGGGGGACGGTGTGTGACGACAACTT
  CAGCAAAGATCACGCTTCTGTGATTTGTAAACAGCTTGGATGTGGAAGTGCCATTAGTTTCTCTGGCTCA
  GCTAAATTGGGAGCTGGTTCTGGACCAATCTGGCTCGATGACCTGGCATGCAATGGAAATGAGTCAGCTC
  TCTGGGACTGCAAACACCGGGGATGGGGCAAGCATAACTGTGACCATGCTGAGGATGTCGGTGTGATTTG
  CTTAGAGGGAGCAGATCTGAGCCTGAGACTAGTGGATGGAGTGTCCAGATGTTCAGGAAGATTGGAAGTG
  AGATTCCAAGGAGAATGGGGGACCGTGTGTGATGATAACTGGGATCTCCGGGATGCTTCTGTGGTGTGCA
  AGCAACTGGGATGTCCAACTGCCATCAGTGCCATTGGTCGAGTTAATGCCAGTGAGGGATCTGGACAGAT
  TTGGCTTGACAACATTTCATGCGAAGGACATGAGGCAACTCTTTGGGAGTGTAAACACCAAGAGTGGGGA
  AAGCATTACTGTCATCATAGAGAAGACGCTGGTGTGACATGTTCTGATGGAGCAGATCTGGAACTTAGAC
  TTGTAGGTGGAGGCAGTCGCTGTGCTGGCATTGTGGAGGTGGAGATTCAGAAGCTGACTGGGAAGATGTG
  TAGCCGAGGCTGGACACTGGCAGATGCGGATGTGGTTTGCAGACAGCTTGGATGTGGATCTGCGCTTCAA
  ACCCAGGCTAAGATCTACTCTAAAACTGGGGCAACAAATACGTGGCTCTTTCCTGGATCTTGTAATGGAA
  ATGAAACTACTTTTTGGCAATGCAAAAACTGGCAGTGGGGCGGCCTTTCCTGTGATAATTTCGAAGAAGC
  CAAAGTTACCTGCTCAGGCCACAGGGAACCCAGACTGGTTGGAGGAGAAATCCCATGCTCTGGTCGTGTG
  GAAGTGAAACACGGAGACGTGTGGGGCTCCGTCTGTGATTTTGACTTGTCTCTGGAAGCTGCCAGTGTGG
  TGTGCAGGGAATTACAATGTGGAACAGTCGTCTCTATCCTAGGGGGAGCACATTTTGGAGAAGGAAGTGG
  ACAGATCTGGGGTGAAGAATTCCAGTGTAGTGGGGATGAGTCCCATCTTTCACTATGCTCAGTGGCGCCC
  CCGCTAGACAGAACTTGTACCCACAGCAGGGATGTCAGCGTAGTCTGCTCACGATACATAGATATTCGTC
  TGGCAGGCGGCGAGTCCTCCTGTGAGGGAAGAGTGGAGCTCAAGACACTCGGAGCCTGGGGTCCCCTCTG
  CAGTTCTCATTGGGACATGGAAGATGCTCATGTCTTATGTCAGCAGCTGAAGTGTGGGGTTGCCCAATCT
  ATTCCAGAAGGAGCACATTTTGGGAAAGGAGCTGGTCAGGTCTGGAGTCACATGTTCCACTGCACTGGAA
  CTGAGGAACATATAGGAGATTGCCTCATGACTGCTCTGGGTGCGCCGACGTGTTCCGAAGGACAGGTGGC
  CTCTGTCATCTGCTCAGGAAACCAATCCCAGACACTATTGCCATGTAGTTCATTGTCTCCAGTCCAAACA
  ACAAGCTCTACAATTCCAAAGGAGAGTGAAGTTCCCTGCATAGCAAGTGGCCAGCTTCGCTTGGTAGGTG
  GAGGTGGTCGCTGCGCTGGAAGAGTGGAGGTCTACCACGAGGGCTCTTGGGGCACCGTCTGTGATGACAA
  TTGGGATATGACTGATGCCAATGTGGTGTGCAAGCAGCTGGACTGTGGCGTGGCAATTAACGCCACTGGC
  TCTGCTTACTTCGGGGAAGGAGCAGGAGCTATCTGGCTAGACGAAGTCATCTGCACTGGGAAAGAGTCTC
  ATATTTGGCAGTGCCATTCACATGGCTGGGGACGCCATAACTGCAGGCACAAAGAAGATGCAGGTGTTAT
  CTGCTCCGAGTTCATGTCTCTGAGGCTGACCAACGAAGCCCACAAAGAAAACTGCACAGGTCGCCTTGAA
  GTGTTTTACAATGGTACATGGGGCAGTATTGGCAGTAGCAATATGTCTCCAACCACTGTGGGGGTGGTGT
  GCCGTCAGCTGGGCTGTGCAGACAACGGGACTGTGAAACCCATACCTTCAGACAAGACACCATCCAGGCC
  CATGTGGGTAGATCGTGTGCAGTGTCCAAAAGGAGTTGACACTTTGTGGCAGTGCCCCTCGTCACCTTGG
  AAACAGAGACAGGCCAGCCCCTCCTCCCAGGAGTCCTGGATCATCTGTGACAACAAAATAAGACTCCAGG
  AAGGGCATACAGACTGTTCTGGACGTGTGGAGATCTGGCACAAAGGTTCCTGGGGAACAGTGTGTGATGA
  CTCCTGGGATCTTAATGATGCTAAGGTTGTATGTAAGCAGTTGGGCTGTGGCCAAGCTGTGAAGGCACTA
  AAAGAAGCAGCATTTGGTCCAGGAACTGGGCCCATATGGCTCAATGAAATTAAGTGTAGAGGGAATGAGT
  CTTCCCTGTGGGATTGTCCTGCCAAACCGTGGAGTCACAGCGACTGTGGGCACAAAGAAGATGCTTCCAT
  CCAGTGCCTCCCAAAAATGACTTCAGAATCACATCATGGCACAGGTCACCCCACCCTCACGGCACTCTTG
  GTTTGTGGAGCCATTCTATTGGTCCTCCTCATTGTCTTCCTCCTGTGGACTCTGAAGCGACGACAGATTC
  AGCGACTTACAGTTTCCTCAAGAGGAGAGGTCTTGATACATCAAGTTCAGTACCAAGAGATGGATTCAAA
  GGCGGATGATCTGGACTTGCTGAAATCCTCGGGGGTCATTCAGAGGCACACTGAGAAGGAAAATGATAAT
  TTATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_053094
• Insert Size: 3366 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053094.2, NP_444324.2
• RefSeq Size: 4409 bp
• RefSeq ORF: 3366 bp
• Locus ID: 93671
• UniProt ID: Q2VLH6
• Cytogenetics: 6 F2
• Gene Summary: Involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1 (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate segment in the 3' coding region, compared to variant 1. The resulting protein (isoform 2) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.