Ccdc36 (NM_001135198) Mouse Tagged ORF Clone

CAT#: MR219067

Ccdc36 (Myc-DDK-tagged) - Mouse coiled-coil domain containing 36 (Ccdc36)

Product Images

Circular map for MR219067

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ccdc36
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR219067 representing NM_001135198
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATTTTAATGTCTGGAATGTTAAAGAGATGCTCAGCATCCCCTCAGGCTCTGGGATCACTAAACCAT
  CTAACTGGAATAATAATCAGACTGACTGTTCTCTCAGTGATTCCCAGTTCCTCTTTGGATCTCAGTTCTG
  TCCTGAAAATTCAGAGACTCTGCTACCGTCCTTGGATGCTGGTGCTTGCTTAAGACACCCAAAACAGACA
  CAACAGAATTCTGTGGATAGTGAACCTAGTATTTTCATAAAATACCAGGCAAAGCCCCAGTTGCTTGGAG
  GCGATACAAAAGATGAAAGCTTATTTTCTCTTCCTTTGCCAGTTGGGAAATCAAAAGGACTCTCAAAACA
  GTTTGAGGAGAAAAAACGAAGGGCAACGGACCAATCTGACAGTGAGACCCTGCACAGCTTTGTTTCTCAT
  TTTCCAGAAGTCATTAACAAGTTGCAGACATCTGTGGAAAAGACTGAGGAAAATCTCAGCTCTAGAAGCC
  AGTCTATTTTAGATTCTGTGGAGACCATAGCCAAGACTTTTCAAGAGACTGCACGGGTCCAGCACGACCT
  GATGGTTGAGTCAGTGAGAGACAAAGGCAGCATGGAGCAGGCCATCCTTGAGATCCAGCGGACATGTGCA
  GCTAGACAAGCAGAATTTATGGAGATGAAATCCACCCTGAAGAACCTTGAAGTTTTAGTTGTGGAGCAGA
  CTAAGAACTTGCAGCAGTTTTGTGACAACCTGAGCCAGCTCATTGTGCCTGGTATCCTAGAAGAGCTGAA
  AAAATTCACCTCTGTGCCTCAGGTGGCTGGGCATCTGAAAGACAGCACCTCTCAGACCTCACCATCTCTG
  ACCCAGAGCCTCCATTTTACCAGGCAGGAAAAGCATCCCTCTGAGGAGCCAGCTACTTGGCAGGCCCAGG
  AGGCTCCTGCAGGAAATCCCAGTACGAGTTCCCAGAGGCCTGGGGAGTGTGGTGTCTGGGATGAAGGAGC
  AGAGAGTGGTGTTTTTCAAAAGGCTGCATTGCCAACAGATGGGCTCCATAGAGGCGATGGGCATGTAAAG
  AACAAGACCGTGCCGACTTACTGCAAGAACTGGGTCATGACCACCAGAAGCGTCAGTAACCACTTCTCTA
  ACCTCCCAAGCCAGAGGGCTGGCAATGGCCAAGGTCTGATGGCCCAAGGAGCTTCACAGCGGGACGTAAG
  TAAGTTTGAAGCCAGAGTGAAGAATGCCTGCCCCGAATATGGGCCCCAAAGCATGTGTTCTTTTGACTCA
  CTTGAACAGTCAGCAACCGAACAGAAAGGCAGGCCCTGCAGAAAAAGGAGGAGAGGCAAGAAGCAACAAC
  CCCAGAGATCAAAGAGAGGTGGGCTTCTAGATAGGAAGCAAGGACAGACCTCAAAGGCGGCCTGTGCTTT
  CATAGCTAGGCATCACTGCCCCCAGTCTCCAGTGTGTGATCCACAGGGGCCCCTCATCTGTTGGCTGACT
  CCGAGAAGTTCCACAAAGTCAACCTGTCACATTTTGGGAGGAACGGGAGAGACCAGTCAGACAGCAAGGG
  CAGCACAAGGGAACCTCGTGCAGCATTCCCAGCGCTCTTCCACAGACAGCAGCTCCCAGGGAGACCAGCA
  GATAAATTGGTTCAGTGACCTCAGCCTTGAAAACCTAGAGCCGCCACAGTGTAAGAAGGGAGGGACGAAT
  TTGCTCTGTGACCCGGATTTTGATAGCAGCGATGATAATTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR219067 representing NM_001135198
  Red=Cloning site Green=Tags(s)
  
  MNFNVWNVKEMLSIPSGSGITKPSNWNNNQTDCSLSDSQFLFGSQFCPENSETLLPSLDAGACLRHPKQT
  QQNSVDSEPSIFIKYQAKPQLLGGDTKDESLFSLPLPVGKSKGLSKQFEEKKRRATDQSDSETLHSFVSH
  FPEVINKLQTSVEKTEENLSSRSQSILDSVETIAKTFQETARVQHDLMVESVRDKGSMEQAILEIQRTCA
  ARQAEFMEMKSTLKNLEVLVVEQTKNLQQFCDNLSQLIVPGILEELKKFTSVPQVAGHLKDSTSQTSPSL
  TQSLHFTRQEKHPSEEPATWQAQEAPAGNPSTSSQRPGECGVWDEGAESGVFQKAALPTDGLHRGDGHVK
  NKTVPTYCKNWVMTTRSVSNHFSNLPSQRAGNGQGLMAQGASQRDVSKFEARVKNACPEYGPQSMCSFDS
  LEQSATEQKGRPCRKRRRGKKQQPQRSKRGGLLDRKQGQTSKAACAFIARHHCPQSPVCDPQGPLICWLT
  PRSSTKSTCHILGGTGETSQTARAAQGNLVQHSQRSSTDSSSQGDQQINWFSDLSLENLEPPQCKKGGTN
  LLCDPDFDSSDDNF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001135198
• ORF Size: 1722 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001135198.2
• RefSeq Size: 2878 bp
• RefSeq ORF: 1725 bp
• Locus ID: 434438
• UniProt ID: Q6PDM4
• Cytogenetics: 9 F2
• MW: 63.3 kDa
• Gene Summary: Required for DNA double-strand breaks (DSBs) formation in unsynapsed regions during meiotic recombination (PubMed:27723721). Probably acts by forming a complex with MEI4 and REC114, which activates DSBs formation in unsynapsed regions, an essential step to ensure completion of synapsis (PubMed:27723721). Not required for HORMAD1 functions in pairing-independent synaptonemal complex formation, ATR recruitment to unsynapsed axes, meiotic silencing of unsynapsed chromatin (MSUC) or meiotic surveillance (PubMed:27723721).[UniProtKB/Swiss-Prot Function]